Production of High Added-Value Chemicals in Basfia succiniciproducens: Role of Medium Composition

Abstract

Succinic acid production through biological fermentation led to new pathways in the integration of renewable feedstock from different industries into biosynthesis. In this article, we investigate the population growth dynamics and succinic acid production potential of the recently isolated natural succinic acid producer, Basfia succiniciproducens, using in silico constraint-based metabolic models as well as in vitro experiments. Our work focuses on the influence of different renewable substrates and added yeast extract on fermentation dynamics, and the produced metabolites of the strain cultured in mineral (minimal) medium. According to our experiments, which were carried out as small-scale fermentations and in bioreactor conditions, glucose is the preferred carbon source, while the addition of 1% yeast extract has a significant positive effect on biomass formation. In the case of B. succiniciproducens cultured in minimal salt medium, a production potential as high as 47.09 mM succinic acid was obtained in these conditions. Industrial applications related to this bacterial strain could contribute to new possibilities for the re-use of byproducts by using fermentation processes, leading to high added-value compounds.

Isolation and screening of microorganisms for high yield of succinic acid production

“…YE and carbon source significantly affected the SA and LA production. 3,33 It was found that B. succiniciproducens cultured in medium with glucose as a carbon source produced higher SA and other acids than medium with xylose and glycerol. However, LA was barely produced in the case of glycerol, and FA was not found in the case of xylose.…”

“…However, LA was barely produced in the case of glycerol, and FA was not found in the case of xylose. 3 A cheap substrate from industrial wastewater could be an alternative source for SA with low levels of unwanted acids.…”

“…Several microorganisms produce SA as their one metabolic products, including Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Corynebacterium glutamicum, Klebsiella pneumoniae, Enterococcus faecalis, Ruminococcus albus, and Basfia succiniciproducens. [3][4][5][6][7] SA production not only can be done by natural SA producers but also by using engineered strains. 8,9 However, A. succinogenes is thought to be the greatest natural SA producers due to high SA production, capability to consume a broad spectrum of cheap substrates, and tolerance under high-stress condition.…”

“…SA belongs to the dicarboxylic family that is also known as butanedioic acid and amber 1 . A wide range of applications in various sectors, including food, industrial, agricultural, pharmaceutical, biodegradable polymers production, and many more, made SA hit in the top 10 value‐added products in USDOE (United State Department of Energy) 2,3 . Awareness of the global demand for bio‐based products urged scientists to investigate different studies to find out all possible ways that can replace petrochemical products.…”

“…Therefore, scientists are looking forward to finding out the biological way of SA production. Several microorganisms produce SA as their one metabolic products, including Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Corynebacterium glutamicum, Klebsiella pneumoniae, Enterococcus faecalis, Ruminococcus albus , and Basfia succiniciproducens 3–7 . SA production not only can be done by natural SA producers but also by using engineered strains 8,9 .…”