Bioprocess optimization for lactic and succinic acid production from a pulp and paper industry side stream

Abstract

The effective and cheap production of platform chemicals is a crucial step towards the transition to a bio-based economy. In this work, biotechnological methods using sustainable, cheap, and readily available raw materials bring bio-economy and industrial microbiology together: Microbial production of two platform chemicals is demonstrated [lactic (LA) and succinic acid (SA)] from a non-expensive side stream of pulp and paper industry (fibre sludge) proposing a sustainable way to valorize it towards economically important monomers for bioplastics formation. This work showed a promising new route for their microbial production which can pave the way for new market expectations within the circular economy principles. Fibre sludge was enzymatically hydrolysed for 72 h to generate a glucose rich hydrolysate (100 g·L−1 glucose content) to serve as fermentation medium for Bacillus coagulans A 541, A162 strains and Actinobacillus succinogenis B1, as well as Basfia succiniciproducens B2. All microorganisms were investigated in batch fermentations, showing the ability to produce either lactic or succinic acid, respectively. The highest yield and productivities for lactic production were 0.99 g·g−1 and 3.75 g·L−1·h−1 whereas the succinic acid production stabilized at 0.77 g·g−1 and 1.16 g·L−1·h−1.

Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry

“…In the year 2023, B. succiniciproducens ATCC 22022 was evaluated to valorize glucose-rich enzymatic hydrolysate derived from sulphite derived sludge, a side waste product of paper and pulp industry. Under batch mode, the strain was able to produce 30.6 g/L SA with yield and productivity being 0.52 g/g and 0.63 g/L/h, respectively, when hydrolysate was fortified with yeast extract [84].…”

Postępy w rozwoju narzędzi biologii syntetycznej do bioprodukcji analogów produktów naturalnych

“…Manipulacja genetyczna prowadzona była mechanizmem egzogennym, który był stymulowany naświetlaniem bakterii Streptomyces promieniowaniem w zakresie ultrafioletu oraz plazmą atmosferyczną o temperaturze pokojowej (ARTP). Dzięki temu pozyskano odmianę wysoko sprawnego mutanta, u którego produkcja takrolimusu wzrosła z 501 do 670 mg/l [40]. Podjęto także próby manipulacji genomu w celu weryfikacji skuteczności mutagenezy endogennej w odniesieniu do takrolimusu.…”