Production of gill-associated and serum antibody byrainbow trout (Oncorhynchus mykiss) following immersion immunization with acetone-killed Flavobacterium branchiophilum and the relationship to protection from experimental challenge

The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: an ontogenetic study

“…Additionally, significant numbers of 'constitutive' ASC were detected in the gills of dab (Limanda limanda), although the peak of specific anti-HGG ASC in intraperitoneally immunised or orally intubated dab accounted for less than 8% of the total ASC (Davidson et al, 1997). The present data and that of Lumsden et al (1993Lumsden et al ( , 1995 suggest the importance of the route of immunisation to locally stimulate the production of specific ASC in the gill. Furthermore, they show the importance that the gills might have in specific antibody responses instead of being an organ better deployed in the production of a wide-ranging immunological barrier to infection in the form of 'constitutive' antibody secretion as hypothesised by Davidson et al (1997).…”

“…This indicates the importance of the gills in immersion vaccination. Specific antibodies have been detected in the gills of brook trout and rainbow trout following 70 immersion infection or immunisation with Flavobacterium branchiophilium, respectively (Lumsden et al, 1993(Lumsden et al, , 1995. These authors showed that immersion vaccinated rainbow trout were better protected against experimental bath challenge than i.p.…”

Immunological aspects of amoebic gill disease in salmonids

“…was used was thought to be the absence of virulence factors in the cultured organisms it is difficult to explain why a crude vaccine containing antigens from virulent organisms did not work. It was shown from the findings of Lumsden et al (1994), who injected (i.p. with 0.1 ml) and immersed (in a 1:10 dilution of acetonekilled Flavobacterium branchiophilum) rainbow trout, that the level of antibody to the bacterium was highest in the i.p.…”

Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation

“…However, positive cells were present in these organs at relatively later stages of development, which may indicate their limited protective roles in earlier stages of fish juveniles. Mucosal Ig-positive cells in gills were analysed in several fish species, and these molecules may be derived from blood or generated locally (Lumsden et al, 1995;Hatten et al, 2001). However, a recent report on dendritic-like cells within gills of fish (Lovy et al, 2006) and the presence of IgM-producing cells also in this organ as identified in the present study may suggest that fish gills are indeed MALT in being able to produce antibodies locally.…”