Production of Gamma Linolenic Acid in Seeds of Transgenic Soybean

Sustainable Production of Omega-3 Fatty Acids

“…Surprisingly co-expression of these two desaturases together with a third gene, the M. alpina Δ12-desaturase, did yield only up to 16 % SDA, suggesting that two desaturases, a Δ6 and a Δ15-desaturase, are sufficient to achieve significant SDA levels in canola oil. In the same year Clemente et al (2003) reported on soybean expressing the borage Δ6-desaturase under the control of the seed-specific beta-conglycinin promoter. The best performing line in this experiment produced up to 5.1 % SDA.…”

“…SDA accumulation was first reported in Canola (Ursin 2003) and in soybean (Clemente et al 2003). Co-expression of the Mortierella alpina Δ6-desaturase and the Brassica napus Δ15-desaturase resulted in canola oil that contained up to 23 % SDA.…”

Production of γ‐Linolenic Acid and Stearidonic Acid in Seeds of Marker‐Free Transgenic Soybean1

“…11Note: A preliminary report of this research has previously been published. See Clemente et al (2003) This publication is a contribution of the University of Nebraska Agricultural Research Division, Lincoln, NE, Journal Series No. 14166.…”

Toward sustainable production of value‐added bioenergy and industrial oils in oilseed and biomass feedstocks

“…As described above, extensive research has focused on the reconstitution of unusual fatty acid biosynthesis and increased TAG assembly in oilseed crops and model plants. However, with only a few exceptions (e.g., γ-linolenic acid, omega-7 monounsaturated fatty acids; Clemente et al, 2003;Nguyen et al, 2015;Qin et al, 2012), the accumulation of unusual fatty acids in transgenic oilseeds has yet to reach levels found in seeds of plants that "naturally" produce unusual fatty acids (Broun & Somerville, 1997;Cahoon et al, 2006). Non-desirable plant fitness phenotypes, such as poor seed germination, impaired seedling establishment, and low total seed oil content, have also been reported to accompany the introduction of at least several of these pathways into plants (Cahoon et al, 2006;van Erp et al, 2011;Jaworski & Cahoon, 2003).…”