Production of fungal lipases using wheat bran and soybean bran and incorporation of sugarcane bagasse as a co-substrate in solid-state fermentation

Fleuri, Luciana Francisco; Oliveira, Mariana Cassani de; Arcuri, Mariana de Lara Campos; Capoville, Bruna L.; Pereira, Milene Stefani; Okino‐Delgado, Clarissa Hamaio; Novelli, Paula Kern

doi:10.1007/s10068-014-0164-7

Cited by 30 publications

(13 citation statements)

References 20 publications

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“…Mala et al [112] conducted an extensive study to optimize the solid-state fermentation process using Aspergillus niger MTCC 2594 and agro-industrial wastes as a substrate, such as wheat bran and gingelly oil cake. The lipase production showed the highest activity of 384.3 U/g of dry substrate containing wheat bran and gingelly oil cake in the ratio of 3:1 w/w at 30 • C over a period of 72 h. Fleuri et al [113] evaluated the feasibility of using 10 fungal strains fermented in wheat bran, soybean bran and sugarcane bagasse for lipase production. No fungal growth was observed using sugarcane bagasse, but the supplementation of the culture medium with wheat bran and soybean bran produced lipase production.…”

Section: Fibrous Residuesmentioning

confidence: 99%

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

et al. 2021

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Lipases are enzymes that catalyze various types of reactions and have versatile applications. Additionally, lipases are the most widely used class of enzymes in biotechnology and organic chemistry. Lipases can be produced by a wide range of organisms including animals, plants and microorganisms. Microbial lipases are more stable, they have substrate specificity and a lower production cost as compared to other sources of these enzymes. Although commercially available lipases are widely used as biocatalysts, there are still many challenges concerning the production of microbial lipases with the use of renewable sources as the main component of microbial growth medium such as straw, bran, oil cakes and industrial effluents. Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two important technologies for the production of lipases by microorganisms. Therefore, this review focuses on microbial lipases, especially their function, specificity, types and technology production, including the use of renewable agro-industrial residues and waste materials.

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Section: Fibrous Residuesmentioning

confidence: 99%

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

et al. 2021

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“…Kynclova et al (1995) screened various hydrolytic enzymes to meet the special demands. Among the screened microorganisms, fungal lipases turned out to be the best source for lipase as they are thermally stable with high turnover number and are presently receiving attention due to the easy recovery of extracellular enzymes (Kynclova et al 1995;Mahadik et al 2002;Salihu et al 2013;Fleuri et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the selection of an appropriate substrate is an essential step during the production of enzymes in SSF. Various agro-industrial residues such as wheat bran, soybean cake, rice husk, gingelly oil cake, olive oil cake, sugar cane bagasse, babassu oil cake, and sheanut cake were studied (Salihu et al 2013(Salihu et al , 2016Fleuri et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

et al. 2019

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Background: Lipases are serine hydrolases that degrade triglycerides, an attribute that treasures wide applications in biodiesel production, detergent, chemical industries, etc. The most sought after the application is in the high quality and economical production of biodiesel under mild reaction conditions and simplified product separation. For the said application, fungal lipases are ideal catalysts that could effectively catalyze esterification and transesterification reactions with their specific ability to release fatty acids from 1, 3 positions of acylglycerols. Results: In the present work, to facilitate bulk synthesis, lipase production using Aspergillus niger MTCC 872 was studied by solid-state fermentation (SSF). The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. rice husk, cottonseed cake, and red gram husk in various combinations at flask level. Tri-substrate mixture (rice husk, cottonseed cake, and red gram husk) combined in the ratio of 2:1:1 has shown the maximum lipase activity 28.19 U/gds at optimum cultivation conditions of temperature 40°C, moisture content 75% (v/w), pH 6.0 and initial spore concentration of 5.4 million spores per mL. Further studies were performed for scale-up of lipase from flask level to lab scale using tray fermenter. Lipase activity was found to be 24.38 U/gds and 21.62 U/gds for 100 g and 1000 g substrate respectively. Conclusion: This is the first report on the production of lipase from Aspergillus niger MTCC 872 using tri-substrate mixture of rice husk (RH), cottonseed cake (CSC), and red gram husk (RGH). Moreover, comparison between individual, binary, and tri-substrate mixture was carried out for which the highest lipase activity was observed for tri-substrate mixture. In addition, comparable results were found when scale-up was performed using tray fermenter. Thus, the current work signifies usage of agro-industrial residues as substrates for enzyme production by solid-state fermentation process as an effective alternative to submerged fermentation for industrial applications.

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“…The (‘Pera’ variety) orange waste extracts and the commercial porcine pancreatic lipase (Sigma-Aldrich ® ) were used in the hydrolysis of crude soybean oils and soybean oil wastes using 5% lipase extract over the reaction medium volume. Crude oil- and waste-reagent blanks were prepared [ 21 ]. The hydrolysis rate was calculated by taking into consideration the NaOH volume used in the titration (mL), multiplied by the concentration and molar mass of the base.…”

Section: Methodsmentioning

confidence: 99%

Bioremediation of cooking oil waste using lipases from wastes

et al. 2017

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Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.

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Production of fungal lipases using wheat bran and soybean bran and incorporation of sugarcane bagasse as a co-substrate in solid-state fermentation

Cited by 30 publications

References 20 publications

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

Bioremediation of cooking oil waste using lipases from wastes

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