“…The chromatographic separation of the target compound was carried out on a reversed-phase column (Luna® C8 column, 100 × 4.6 mm, 3 μm) at 30 • C. The mobile phasethe mixture of water (A) and methanol (B) -was pumped at a flow rate of 0.8 mL min − 1 . The gradient elution was programmed using the following pattern: 0-20 min, 20 %-0 % A; 20-25 min, 0 % A; 25-27 min, 0 %-20 % A; 27-30 min, 20 % A [18,24]. The HPLC analysis of samples was monitored at 440 nm.…”