Cold-adapted culturing of the microalga Monoraphidium sp. in thin-layer raceway pond for biomass production

Lakatos, Gergely; Ranglová, Karolína; Bárcenas-Pérez, Daniela; Grivalský, Tomáš; Manoel, João Câmara; Mylenko, Mykola; Cheel, José; Nyári, József; Wirth, Roland; Kovács, Kornél L.; Kopeckỳ, J.; Nedbalová, Linda; Masojídek, Jiří

doi:10.1016/j.algal.2022.102926

Cited by 6 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure S6, 18S rRNA gene sequencing analysis showed that light mainly promoted the growth of Chlorophyta (99.79%). Among these, Parachlorella (59.91%) dominated, followed by Monoraphidium (23.37%) and Desmodesmus (16.45%), which have been reported to have their growth promoted by light. , …”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Reconsidering the Rationality of Ultrafiltration as the Ultimate Safeguard for Reclaimed Water Biosafety: Unexpected Risks Arise under Different Storage Conditions

Cui,

Ding,

et al. 2024

ACS EST Water

View full text Add to dashboard Cite

Ultrafiltration, an advanced process for producing biologically safe reclaimed water, has confronted challenges from bacterial regrowth. We conducted a comprehensive investigation into the biosafety risks of ultrafiltration-treated reclaimed water under five distinct storage conditions, considering temperature variations, airborne bacterial invasion, and light. Although ultrafiltration removed over 97% of the bacterial load, bacterial regrowth occurred during storage. Elevated temperatures resulted in the highest heterotrophic plate count (HPC), while lower temperatures favored increased total cell count (TCC). Notably, lower temperatures facilitated a surge in the number of potential pathogenic bacteria. Light exposure also elevated the potential pathogenic bacteria. Surprisingly, high temperatures posed minimal biosafety risk. While airborne bacterial invasion insignificantly affected potential pathogenic bacterial count, it constituted 67 ± 4% of total regrowth bacteria, with membrane breakthrough bacteria comprising the remaining 33 ± 4%, as revealed by SourceTracker. The impact of storage conditions on the bacterial community structure varied, with temperature exerting the most significant effect. Correlation analysis affirmed the potential of UV254 and total fluorescence intensity as alternative indicators for HPC, and polysaccharide content emerged as a suitable substitute indicator for potential pathogenic bacterial count. Future endeavors should prioritize the development of integrated ultrafiltration processes designed to inhibit pathogen regrowth in reclaimed water treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Among these, Parachlorella (59.91%) dominated, followed by Monoraphidium (23.37%) and Desmodesmus (16.45%), which have been reported to have their growth promoted by light. 45,46 3.3.2. Differential Analysis of the Bacterial Community under Different Storage Conditions.…”

Section: Microbial Community 331 Composition Of Microbial Communitymentioning

confidence: 99%

Reconsidering the Rationality of Ultrafiltration as the Ultimate Safeguard for Reclaimed Water Biosafety: Unexpected Risks Arise under Different Storage Conditions

Cui,

Ding,

et al. 2024

ACS EST Water

View full text Add to dashboard Cite

show abstract

“…Recently, Monoraphidium sp. has gained recognition as a possible biofuel feedstock because of its adaptability to a variety of environmental factors [31]. However, the potential of Monoraphidium sp.…”

Section: Resultsmentioning

confidence: 99%

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

et al. 2023

View full text Add to dashboard Cite

Carotenoids have attracted significant interest due to their potential use in human health and nutrition, and their global demand increases by 3.7% annually. Currently, synthetic carotenoids dominate the market, but possess challenges such as low antioxidant ability, issues with health benefits, and safety concerns. Microalgae are potential producers of natural carotenoids with extraordinary antioxidant properties, although the yield is often low in their natural cycle. The present investigation aimed to analyze the effect of multiple abiotic factors on enhancing algal carotenoids synthesis and other metabolites without affecting growth. The potential microalgae Monoraphidium sp. were grown under altered nutrient and light conditions employing RSM-CCD. The optimized conditions, such as Nitrogen (5 g·L−1), Phosphorus +Potassium (250 mg·L−1), Sulphur (70 mg·L−1), and light (137.5 µmol·m−2·S−1), resulted in increased biomass (1357.36 mg·L−1), lipid accumulation (40.28% of dry biomass), and carotenoids (16.26 µg·mL−1) as compared to the control conditions. The total carotenoids fraction consisted of astaxanthin (14.8%), violaxanthin (3.61%), lutein, (45.12%), 9-cis-β carotene (7.62%), and β-carotene-5,6-epoxide (24.21%). Among them, violaxanthin (1.32-fold), astaxanthin (1.19-fold), 9-cis- β carotene (1.07-fold), and β-carotene-5,6-epoxide (1.08-fold) content increased while lutein (1.32-fold) content decreased significantly. The improvement in algal carotenoids under novel culture conditions provides a significant advantage to pharmaceutical and nutraceutical industries.

show abstract

“…are found all over the globe and are noted for having a very extensive range of biotic communities [26]. Recently, MP has gained recognition as a possible biofuel feedstock because of its adaptability to a variety of environmental factors [27]. The potential of MP as a source of carotenoids, on the contrary side, has undergone less investigation.…”

Section: Resultsmentioning

confidence: 99%

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Yadav¹,

Kumar²,

Nikalje³

et al. 2023

Preprint

View full text Add to dashboard Cite

Carotenoids are essential pigments that play a vital role in the photosynthetic process of algae and are important for human health and nutrition. Despite their potential value, algal carotenoids are being neglected by biofuel industries. Recent research is aimed at producing microalgal lipids and carotenoids simultaneously under abiotic stresses. In this study, the effect of different abiotic stress factors such as Nitrogen (N), Potassium (K), Phosphorous (P), Sulphur (S), light intensity, and photoperiod on biomass, lipid, and carotenoids synthesis in Monoraphidium sp. (MP) were explored utilizing a statistical method. In addition, 2, 7-dichlorofluorescein diacetate (DCF-DA) mediated spectrofluorimetry was employed to detect the ele-vation in ROS level. The results revealed that variations in nutrients and light con-ditions are the key factors in the up-regulation of biomolecules lipids and carote-noids in the algae. The carotenoids profiling was done by Ultra Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectroscopy (UPLC-Q-ToF-MS). Among consisting carotenoids violaxanthin, astaxanthin, and beta-carotene were enhanced by 1.3 folds, 1.19 folds, 1.08 folds, while lutein was declined by 1.32 folds under stressed condition compared to control. The identified carotenoids are rec-ognized for their importance in the pharmaceutical and nutraceutical industries, highlighting the significant role of MP in these sectors.

show abstract

Cold-adapted culturing of the microalga Monoraphidium sp. in thin-layer raceway pond for biomass production

Cited by 6 publications

References 48 publications

Reconsidering the Rationality of Ultrafiltration as the Ultimate Safeguard for Reclaimed Water Biosafety: Unexpected Risks Arise under Different Storage Conditions

Reconsidering the Rationality of Ultrafiltration as the Ultimate Safeguard for Reclaimed Water Biosafety: Unexpected Risks Arise under Different Storage Conditions

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Contact Info

Product

Resources

About