Production of fibrolytic enzymes by Aspergillus japonicus C03 using agro-industrial residues with potential application as additives in animal feed

Abstract

Solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by Aspergillus japonicus C03. Regarding the enzymatic production the highest levels were observed at 30 °C, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. Peptone improved the xylanase and cellulase activities in 12 and 29%, respectively. The optimum temperature corresponded to 60 °C and 50-55 °C for xylanase and cellulase, respectively, both having 4.0 as optimum pH. Xylanase was fully stable up to 40 °C, which is close to the rumen temperature. The enzymes were stable in pH 4.0-7.0. Cu++ and Mn++ increased xylanase and cellulase activities by 10 and 64%, respectively. A. japonicus C03 xylanase was greatly stable in goat rumen fluid for 4 h during in vivo and in vitro experiments.

Optimization of Multi-enzyme Production by Fungi Isolated from Palm Kernel Expeller using Response Surface Methodology

“…Nevertheless, the efficient production of this enzyme mixture is dependent on various factors such as temperature, moisture, pH, and inocula (Abdel-Sater and El-Said 2001;Facchini et al 2011). Thus, it is important to optimize the production system to enhance the enzyme production capability of the isolated fungal strain.…”

“…Temperature is one of the main factors affecting the growth of fungi, although these microorganisms have been shown to be able to tolerate a wide range of temperatures, typically from 30 to 40 °C, and some are able to survive in extreme temperatures (≥ 50 °C) (Michael 1972;Smith and Wood 1991;Lin and Chen 2004;Wang et al 2006;Chellapandi and Jani 2009;Sohail et al 2009;Facchini et al 2011). Despite the wide temperature tolerance, the optimum temperature predicted was 30 °C, a temperature that is close to the natural habitat where this fungus was isolated.…”

Cellulase Production from Species of Fungi and Bacteria from Agricultural Wastes and Its Utilization in Industry: A Review

“…Moreover, this enzyme is capable to bear the pH change of 4.0 -7.0. It is also observed that Manganese and Copper enhanced the cellulase activity up to 64% [40].…”

Phân Lập Và Đánh Giá Đặc Tính Chủng Aspergillus Brunneoviolaceus Fec 156 Sinh Tổng Hợp Hệ Enzyme Thủy Phân Xylan

“…Trong nghiên cứu của Leda và cộng sự (2013), chủng Aspergillus japonicas cũng sinh tổng hợp xylanase hoạt lực cao với cơ chất cám mì [10]. Còn đối với môi trường nuôi cấy dạng rắn (solid state), chủng A. japonicus C03 lại có khả năng sinh xylanase tốt nhất trên môi trường cám đậu tương (224,6 IU/ml), sau đó mới đến cám mì (155,5 IU/ml) [11]. Kết quả nghiên cứu ban đầu cho thấy A. brunneoviolaceus FEC 156 là một chủng có tiềm năng và cần được nghiên cứu sâu hơn cho các ứng dụng trong chế biến phế phụ phẩm nông, lâm nghiệp.…”