Production of fatty acid methyl esters and other bioactive compounds in elicited cultures of the fungus Mucor circinelloides

Sánchez-Pujante, Pedro Joaquín; Miras-Moreno, Begoña; Soluyanova, Polina; Garre, Victoriano; Pedreño, María A.; Almagro, Lorena

doi:10.1007/s11557-017-1278-0

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…This is the first study to report the carotenogenesis within this strain (WJ11). Lipid and β-carotene are initially produced from the same pool of acetyl-CoA and then further proceed through FAS and Mevalonate pathway [30]. To determine how does upregulation of the mevalonate pathway under light affected mevalonate flux, the expression of key genes hmgS, carG, carB, and carRP, involved in β-carotene biosynthesis were quantified by reverse-transcription quantitative PCR (RT-qPCR).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions

Naz

Nosheen

et al. 2020

Metabolites

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Carotenoids are natural potent antioxidants and free radical scavengers which are able to modulate the pathogenesis of some cancers and heart diseases in human, indicating their importance in being provided through the diet. Mucor circinelloides accumulates β-carotene as the main carotenoid compound and has been used as a model organism in carotenogenic studies. In the present study, the potential of two M. circinelloides strains to accumulate β-carotene was investigated under light and dark conditions. The results, which were quantitated by HPLC, showed that CBS 277.49 accumulated higher pigment in comparison to WJ11 under both conditions. Continuous illumination triggered the pigment accumulation up to 2.7-fold in strain CBS 277.49 and 2.2-fold in strain WJ11 in comparison to dark. The mRNA analysis of the four key genes involved in isoprenoid pathway by RT-qPCR showed higher transcriptional levels in CBS 277.49 as compared to WJ11, indicating that the pigment production metabolic machinery is more active in CBS 277.49 strain. A new scope for further research was established by this work for improved β-carotene production in the high producing strain CBS 277.49.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions

Naz

Nosheen

et al. 2020

Metabolites

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“…It has been described in the literature that carotenoid and ergosterol are part of the highly conserved mevalonate pathway in M. circinelloides [ 41 ]. These two-branched pathways share FPP, which is a common precursor of sterol and β -carotene synthesis.…”

Section: Resultsmentioning

confidence: 99%

“…Thakur and Azmi reported 3.4 mg/L of β -carotene production in Mucor azygosporus MTCC 414 by cultivating it on malt yeast peptone medium [ 28 ]. In another investigation, β -carotene production of nearly 14 μ g/g by M. circinelloides was achieved [ 41 ]. Zhang et al reported that the nonmodified strain of M. circinelloides R7B produced 170.8 μ g/g of β -carotene [ 11 ].…”

Section: Resultsmentioning

confidence: 99%

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Naz

Nazir

Nosheen

et al. 2020

BioMed Research International

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Carotenoids produced by microbial sources are of industrial and medicinal importance due to their antioxidant and anticancer properties. In the current study, optimization of β-carotene production in M. circinelloides strain 277.49 was achieved using response surface methodology (RSM). Cerulenin and ketoconazole were used to inhibit fatty acids and the sterol biosynthesis pathway, respectively, in order to enhance β-carotene production by diverting metabolic pool towards the mevalonate pathway. All three variables used in screening experiments were found to be significant for the production of β-carotene. The synergistic effect of the C/N ratio, cerulenin, and ketoconazole was further evaluated and optimized for superior β-carotene production using central composite design of RSM. Our results found that the synergistic combination of C/N ratios, cerulenin, and ketoconazole at different concentrations affected the β-carotene productions significantly. The optimal production medium (std. order 11) composed of C/N 25, 10 μg/mL cerulenin, and 150 mg/L ketoconazole, producing maximum β-carotene of 4.26 mg/L (0.43 mg/g) which was 157% greater in comparison to unoptimized medium (1.68 mg/L, 0.17 mg/g). So, it was concluded that metabolic flux had been successfully redirected towards the mevalonate pathway for enhanced β-carotene production in CBS 277.49.

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Use of cyclodextrins to improve the production of plant bioactive compounds

Almagro

Pedreño

2020

Phytochem Rev

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Production of fatty acid methyl esters and other bioactive compounds in elicited cultures of the fungus Mucor circinelloides

Cited by 3 publications

References 19 publications

Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions

Comparative Analysis of β-Carotene Production by Mucor circinelloides Strains CBS 277.49 and WJ11 under Light and Dark Conditions

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Use of cyclodextrins to improve the production of plant bioactive compounds

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