Use of cyclodextrins to improve the production of plant bioactive compounds

Almagro, Lorena; Pedreño, María A.

doi:10.1007/s11101-020-09704-6

Cited by 17 publications

(8 citation statements)

References 84 publications

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“…However, future research is also focused on CDs properties and their interaction with other materials (e.g., durability, stability, or dispersion), new applications (e.g., CDs immobilization for wastewater treatment) or the development of green chemistry processes [ 14 ]. They have also been used for sequester certain molecules (i.e., cholesterol), as modulators for medicinal biomaterials, as elicitors or as synergistic agents for the production of secondary metabolites in plants [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host–Guest Complexes

Pereira

Carpena

García-Oliveira

et al. 2021

IJMS

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Cyclodextrins (CDs) are cyclic oligomers broadly used in food manufacturing as food additives for different purposes, e.g., to improve sensorial qualities, shelf life, and sequestration of components. In this review, the latest advancements of their applications along with the characteristics of the uses of the different CDs (α, β, γ and their derivatives) were reviewed. Their beneficial effects can be achieved by mixing small amounts of CDs with the target material to be stabilized. Essentially, they have the capacity to form stable inclusion complexes with sensitive lipophilic nutrients and constituents of flavor and taste. Their toxicity has been also studied, showing that CDs are innocuous in oral administration. A review of the current legislation was also carried out, showing a general trend towards a wider acceptance of CDs as food additives. Suitable and cost-effective procedures for the manufacture of CDs have progressed, and nowadays it is possible to obtain realistic prices and used them in foods. Therefore, CDs have a promising future due to consumer demand for healthy and functional products.

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Section: Introductionmentioning

confidence: 99%

Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host–Guest Complexes

Pereira

Carpena

García-Oliveira

et al. 2021

IJMS

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“…According to these results, CD increased the levels of triterpenes in the extracellular medium due to their structural properties since they can trap triterpenes in their hydrophobic central cavity, forming an inclusion complex [ 38 ]. By isolating the metabolites, CD can protect them from degradation and enhance their release into the culture system [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Cyclodextrins Increase Triterpene Production in Solanum lycopersicum Cell Cultures by Activating Biosynthetic Genes

Sabater-Jara

Marín-Marín²,

Almagro³

et al. 2022

Plants

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In this work, Solanum lycopersicum cv. Micro-Tom suspension-cultured cells were used to analyze the effect of different elicitors including β-cyclodextrins (CD), methyl jasmonate (MJ), β-glucan (Glu) and 3-hexenol (Hex) separately and the combined treatments of CD + MJ, CD + glu and CD + Hex on triterpene compound production after 24, 72 and 96 h. Moreover, we studied the changes induced by elicitors in the expression of key biosynthetic genes to elucidate the regulation of the triterpene biosynthetic pathway. The relative abundance of the triterpene compounds identified in the extracellular medium after elicitation (squalene, fucosterol, avenasterol, β-sitosterol, cycloartenol and taraxasterol) was determined by gas chromatography coupled to mass spectrometry, and the expression level of genes in treated-cells was analyzed by real-time quantitative polymerase chain reaction (qRT-PCR). Results showed that, in CD-treated cells (CD, CD + MJ, CD + Glu, CD + Hex), specialized metabolites were accumulated mainly in the extracellular medium after 72 h of elicitation. Moreover, qRT-PCR analysis revealed that the highest triterpene levels in CD-treated cells (CD, CD + MJ, CD + Glu, CD + Hex) were highly correlated with the expression of cycloartenol synthase, 3-hydroxy-3-methylglutaryl-CoA reductase and squalene epoxidase genes at 24 h of treatment, whereas the expression of sterol methyltransferase was increased at 72 h. According to our findings, CD acts as a true elicitor of triterpene biosynthesis and can promote the release of bioactive compounds from the tomato cells into the extracellular medium. The results obtained provide new insights into the regulation of the triterpene metabolic pathway, which might be useful for implementing metabolic engineering techniques in tomato.

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“…CD are cyclic oligosaccharides, known as drug carriers for their ability to complex, thus solubilizing, various compounds. Nevertheless, they were reported to effectively improve the production of bioactive compounds in plant cell cultures [ 233 ].…”

Section: Plant Suspension Cell Cultures As Bio-factories Of Naturamentioning

confidence: 99%

Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field

Balestrini

Brunetti

Cammareri

et al. 2021

IJMS

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Plant specialized metabolites (SMs) play an important role in the interaction with the environment and are part of the plant defense response. These natural products are volatile, semi-volatile and non-volatile compounds produced from common building blocks deriving from primary metabolic pathways and rapidly evolved to allow a better adaptation of plants to environmental cues. Specialized metabolites include terpenes, flavonoids, alkaloids, glucosinolates, tannins, resins, etc. that can be used as phytochemicals, food additives, flavoring agents and pharmaceutical compounds. This review will be focused on Mediterranean crop plants as a source of SMs, with a special attention on the strategies that can be used to modulate their production, including abiotic stresses, interaction with beneficial soil microorganisms and novel genetic approaches.

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Use of cyclodextrins to improve the production of plant bioactive compounds

Cited by 17 publications

References 84 publications

Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host–Guest Complexes

Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host–Guest Complexes

Cyclodextrins Increase Triterpene Production in Solanum lycopersicum Cell Cultures by Activating Biosynthetic Genes

Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field

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