Production of extracellular silver nanoparticles by radiation-resistant Deinococcus wulumuqiensis R12 and its mechanism perspective

Xiao, Anqi; Wang, Bixuan; Zhū, Lìyǐng; Jiang, Ling

doi:10.1016/j.procbio.2020.10.007

Cited by 7 publications

(7 citation statements)

References 38 publications

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“…The supernatant of wild-type D. radiodurans has been previously used to produce AgNPs, , and similar to other bacteria, enzymes like nitrate reductase and other oxidoreductases are thought to be responsible for silver reduction. , Supporting this, a NADPH-dependent oxidoreductase was found to facilitate AgNP biosynthesis using Deinococcus wulumuqiensis R12 . In addition, D.…”

Section: Resultsmentioning

confidence: 95%

“…13,20 Supporting this, a NADPH-dependent oxidoreductase was found to facilitate AgNP biosynthesis using Deinococcus wulumuqiensis R12. 76 In addition, D. radiodurans possesses an exceptional antioxidative system consisting of antioxidant enzymes and small molecules like pyrroloquinoline−quinone, carotenoids, and manganese complexes that could also play a role in metal reduction. 55,77,78 For example, the carotenoid deinoxanthin was shown to reduce Au(III) to form gold nanoparticles.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Small RNAs as a New Platform for Tuning the Biosynthesis of Silver Nanoparticles for Enhanced Material and Functional Properties

Chen

Hernández-Vargas

Han

et al. 2021

ACS Appl. Mater. Interfaces

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Genetic engineering of nanoparticle biosynthesis in bacteria could help facilitate the production of nanoparticles with enhanced or desired properties. However, this process remains limited due to the lack of mechanistic knowledge regarding specific enzymes and other key biological factors. Herein, we report on the ability of small noncoding RNAs (sRNAs) to affect silver nanoparticle (AgNP) biosynthesis using the supernatant from the bacterium Deinococcus radiodurans. Deletion strains of 12 sRNAs potentially involved in the oxidative stress response were constructed, and the supernatants from these strains were screened for their effect on AgNP biosynthesis. We identified several sRNA deletions that drastically decreased AgNP yield compared to the wild-type (WT) strain, suggesting the importance of these sRNAs in AgNP biosynthesis. Furthermore, AgNPs biosynthesized using the supernatants from three of these sRNA deletion strains demonstrated significantly enhanced antimicrobial and catalytic activities against environmentally relevant dyes and bacteria relative to AgNPs biosynthesized using the WT strain. Characterization of these AgNPs using electron microscopy (EM), energy-dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) revealed that the deletion of these small RNAs led to changes within the supernatant composition that altered AgNP properties such as the surface chemistry, surface potential, and overall composition. Taken together, our results demonstrate that modulating specific sRNA levels can affect the composition of supernatants used to biosynthesize AgNPs, resulting in AgNPs with unique material properties and improved functionality; as such, we introduce sRNAs as a new platform for genetically engineering the biosynthesis of metal nanoparticles using bacteria. Many of the sRNAs examined in this work have potential regulatory roles in oxidative stress responses; further studies into their targets could help provide insight into the specific molecular mechanisms underlying bacterial biosynthesis and metal reduction, enabling the production of nanoparticles with enhanced properties.

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Section: Resultsmentioning

confidence: 95%

Section: ■ Results and Discussionmentioning

confidence: 99%

Small RNAs as a New Platform for Tuning the Biosynthesis of Silver Nanoparticles for Enhanced Material and Functional Properties

Chen

Hernández-Vargas

Han

et al. 2021

ACS Appl. Mater. Interfaces

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“…Figure 1C showed that the FTIR spectrum of C‐AgNPs, was in consistent with the FTIR results reported by Fadaka et al., where prime bands were associated with the C–H stretch and alkenyl C═C stretch. [ 47 ] Compared to C‐AgNPs, the FTIR spectrum of R12‐AgNPs showed more adsorption bands, including 2971, 2921, 1066, and 880 cm −1 , The peaks referred to ─CH 2 bonds, ─C─N stretching the amide group [ 29 ] and ─C═ CH 2 of the aromatic groups. [ 48,49 ] In our case, the cell‐free supernatant of D. wulumuqiensis R12 used for the synthesis of AgNPs was found to contain rich components (Figure S4, Tables S1 and S2), including terpenoids, [ 30 ] proteins, [ 31,50 ] amino acids, nucleoside.…”

Section: Resultsmentioning

confidence: 99%

“…AgNPs were synthesized by using the cell-free supernatant of D. wulumuqiensis R12, and they were labeled as R12-AgNPs. [29] For comparison, another type of AgNPs were produced by utilizing NaBH 4 as the reducing and stabilizing agent, and they were labeled as C-AgNPs. [33] The characterization of AgNPs [34,35] was conducted employing a range of analytical techniques including UV-visible (UV-Vis, Lambda 25) spectroscopy, scanning electron microscope (SEM, SU8200), fourier transform infrared (FTIR, Nicolet iS50), X-ray diffraction (XRD, Miniflex 600), and transmission electron microscope (TEM, JEOL Jem-2100 F).…”

Section: Preparation and Characterization Of Agnpsmentioning

confidence: 99%

Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase‐like activity for synergistic antibacterial application

Liu,

Zhang,

Shen

et al. 2024

Biotechnology Journal

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The use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12‐AgNPs with H2O2 therapy. R12‐AgNPs were simply produced with the supernatant of an ionizing radiation‐tolerant bacterium Deinococcus wulumuqiensis R12 by one‐step under room temperature. In comparison with chemically synthesized AgNPs, the biosynthesized AgNPs presented fascinating antibacterial activity and peroxidase‐like properties, which endowed it with the capability to catalyze the decomposition of H2O2 to generate hydroxyl radical. After the combination of R12‐AgNPs and H2O2, an excellent synergistic bacteriostatic activity was observed for both Escherichia coli and Staphylococcus aureus, especially at low concentrations. In addition, in vitro cytotoxicity tests showed R12‐AgNPs had good biocompatibility. Thus, this work presents a novel antibacterial agent that exhibits favorable synergistic antibacterial activity and low toxicity, without the use of antibiotics or a complicated synthesis process.

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“…The data presented in this study are available in [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ] (MNPs biosynthesis mechanisms), [ 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 ,...…”

mentioning

confidence: 99%

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

et al. 2023

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Nanoparticles (1 to 100 nm) have unique physical and chemical properties, which makes them suitable for application in a vast range of scientific and technological fields. In particular, metal nanoparticle (MNPs) research has been showing promising antimicrobial activities, paving the way for new applications. However, despite some research into their antimicrobial potential, the antimicrobial mechanisms are still not well determined. Nanoparticles’ biosynthesis, using plant extracts or microorganisms, has shown promising results as green alternatives to chemical synthesis; however, the knowledge regarding the mechanisms behind it is neither abundant nor consensual. In this review, findings from studies on the antimicrobial and biosynthesis mechanisms of MNPs were compiled and evidence-based mechanisms proposed. The first revealed the importance of enzymatic disturbance by internalized metal ions, while the second illustrated the role of reducing and negatively charged molecules. Additionally, the main results from recent studies (2018–2022) on the biosynthesis of MNPs using microorganisms were summarized and analyzed, evidencing a prevalence of research on silver nanoparticles synthesized using bacteria aiming toward testing their antimicrobial potential. Finally, a synopsis of studies on MNPs applied to cultural heritage materials showed potential for their future use in preservation.

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Production of extracellular silver nanoparticles by radiation-resistant Deinococcus wulumuqiensis R12 and its mechanism perspective

Cited by 7 publications

References 38 publications

Small RNAs as a New Platform for Tuning the Biosynthesis of Silver Nanoparticles for Enhanced Material and Functional Properties

Small RNAs as a New Platform for Tuning the Biosynthesis of Silver Nanoparticles for Enhanced Material and Functional Properties

Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase‐like activity for synergistic antibacterial application

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

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