Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

López-Linares, Juan Carlos; Gómez‐Cruz, Irene; Ruiz, Encarnación; Romero, Inmaculada; Castro, Eulógio

doi:10.3390/pr8050533

Cited by 21 publications

(9 citation statements)

References 42 publications

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“…For instance, Manzanares et al [20] studied different hydrothermal pretreatments for sugar recovery from both the cellulose and hemicellulose fractions of this waste. López-Linares et al [21] reported a bioconversion process for EOP hemicellulosic sugars into ethanol by fermentation with Escherichia coli; also, the production of xylitol from a hemicellulosic hydrolysate of EOP by Candida boidinii was evaluated [22]. According to Albahari et al [23], the valorisation of the olive oil byproducts is extremely interesting, although it should be based on sustainable principles and green chemistry.…”

Section: Introductionmentioning

confidence: 99%

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

et al. 2020

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Exhausted olive pomace (EOP) is the waste generated from the drying and subsequent extraction of residual oil from the olive pomace. In this work, the effect of different aqueous solvents on the recovery of antioxidant compounds from this lignocellulosic biomass was assessed. Water extraction was selected as the best option for recovering bioactive compounds from EOP, and the influence of the main operational parameters involved in the extraction was evaluated by response surface methodology. Aqueous extraction of EOP under optimised conditions (10% solids, 85 ºC, and 90 min) yielded an extract with concentrations (per g EOP) of phenolic compounds and flavonoids of 44.5 mg gallic acid equivalent and 114.9 mg rutin equivalent, respectively. Hydroxytyrosol was identified as the major phenolic compound in EOP aqueous extracts. Moreover, these extracts showed high antioxidant activity, as well as moderate bactericidal action against some food-borne pathogens. In general, these results indicate the great potential of EOP as a source of bioactive compounds, with potential uses in several industrial applications.

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Section: Introductionmentioning

confidence: 99%

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

et al. 2020

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“…However, fermentation from E. grandis hydrolysate enabled an ethanol yield of 0.21 g ethanol/g sugar [14]. In contrast, López-Linares et al [55] reached ethanol yields of 0.46-0.47 g/g when employing exhausted olive pomace hydrolysate with the bacteria E. coli SL100, with a maximum ethanol concentration of 13.6-14.5 g/L. In the present work, an ethanol yield of 0.31 g ethanol/g consumed sugar was obtained when using the S. stipitis strain, while the recombinant S. cerevisiae MEC1133 produced 0.33 g ethanol/g consumed sugar.…”

Section: Hydrolysates Fermentation For Ethanol Productionmentioning

confidence: 90%

Hemicellulosic Bioethanol Production from Fast-Growing Paulownia Biomass

Domínguez¹,

Río

Romaní

et al. 2021

Processes

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In order to exploit a fast-growing Paulownia hardwood as an energy crop, a xylose-enriched hydrolysate was obtained in this work to increase the ethanol concentration using the hemicellulosic fraction, besides the already widely studied cellulosic fraction. For that, Paulownia elongata x fortunei was submitted to autohydrolysis treatment (210 °C or S0 of 4.08) for the xylan solubilization, mainly as xylooligosaccharides. Afterwards, sequential stages of acid hydrolysis, concentration, and detoxification were evaluated to obtain fermentable sugars. Thus, detoxified and non-detoxified hydrolysates (diluted or not) were fermented for ethanol production using a natural xylose-consuming yeast, Scheffersomyces stipitis CECT 1922, and an industrial Saccharomyces cerevisiae MEC1133 strain, metabolic engineered strain with the xylose reductase/xylitol dehydrogenase pathway. Results from fermentation assays showed that the engineered S. cerevisiae strain produced up to 14.2 g/L of ethanol (corresponding to 0.33 g/g of ethanol yield) using the non-detoxified hydrolysate. Nevertheless, the yeast S. stipitis reached similar values of ethanol, but only in the detoxified hydrolysate. Hence, the fermentation data prove the suitability and robustness of the engineered strain to ferment non-detoxified liquor, and the appropriateness of detoxification of liquor for the use of less robust yeast. In addition, the success of hemicellulose-to-ethanol production obtained in this work shows the Paulownia biomass as a suitable renewable source for ethanol production following a suitable fractionation process within a biorefinery approach.

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“…The OPW were also reused for crude oil production (Pasqualone et al 2016). The energy recovery of OPW can be performed by alcoholic fermentation for bioethanol production (López-Linares et al 2020) or by anaerobic digestion for biogas production (Timpanaro et al 2021), or by thermochemical processes which involve combustion, gasification and pyrolysis (De la Torre Maroto et al, 2020;Dorado et al, 2021;Nunes et al, 2020). Knowing the OPW low biodegradability and the high content of organic matter, the thermochemical conversion of these residues has several advantages over conventional biochemical processes as it helps to reduce the process costs by producing economic-value products applicable in several fields: energetic as biofuels, agronomic as biofertilizers and chemical as source of bioactive molecules.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Valorization of Olive Pomace Waste to Renewable Biofuels, Biomaterials and Biochemicals Via Pyrolysis Process: Experimental and Numerical Investigation

Aissaoui

Trabelsi²,

Bensidhom³

et al. 2021

Preprint

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This work demonstrates, experimentally and numerically, the potential of Olive Pomace Waste (OPW) to produce renewable biofuels (pyrolytic oil and gas), bio-chemicals (tars as source of bioactive molecules) and bio-fertilizers (chars) through slow pyrolysis. Experimental pyrolysis runs were conducted at 500, 600 and 700°C as final pyrolysis temperature, 15, 20 and 25°C/min as heating rate and 1h as residence time, in a fixed bed pyrolyzer. In the optimum pyrolysis conditions (600°C and 15°C/min), 33 wt.% of oil, 30.00 wt.% of char and 37 wt.% of gas were produced. Recovered pyrolytic oil presents good energy value (HHV between 15.96 and 20.94 MJ/kg) with a great bioactive potential. The released permanent gases show an interesting energy content (LHV up to 11 MJ/Kg) which emphasizes their application in a gas engine to provide renewable electricity in rural olive groves area. The recovered OPW biochar presents a high carbon (C 72.54 wt.%) and nutrients contents (up to 8.42 mg/g of Ca, up to 8.69 mg/g of K and up to 2.02 % of total N) which make it suitable for soil amendment and for long-term carbon sequestration. Kinetic study of OPW pyrolysis, performed using the Distributed Activation Energy Model (DAEM), gives an activation energy values ranging from 121.6 to 151.6 kJ/mol. The investigation of the OPW thermal behavior and reactivity under pyrolysis conditions is useful approach to design and operate slow pyrolysis process at commercial scale, which could be useful by farmers for OPW in olive fields.

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Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

Cited by 21 publications

References 42 publications

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

Hemicellulosic Bioethanol Production from Fast-Growing Paulownia Biomass

Sustainable Valorization of Olive Pomace Waste to Renewable Biofuels, Biomaterials and Biochemicals Via Pyrolysis Process: Experimental and Numerical Investigation

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