Production of Enzyme and Protein Hydrolysate from Fish Processing Waste

Prasertsan, Poonsuk; Jitbunjerdkul, S.; Trairatananukoon,; Prachumratana, T.

doi:10.1007/978-94-017-0203-4_7

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…léíáãìã=ée=çÑ=mêçíÉ~ëÉI=iáé~ëÉI=~åÇ=^ãóä~ëÉ= The highest proteolytic activities in both SCO 2 and nhexane treated squid viscera extracts were found at alkaline pH 9 (data not shown). High protease activities at pH ranging from 8.0 to 10.0 have also been reported in several fish species [22][23][24]. Low protease activities were also found in acidic pH.…”

Section: Bññéåí=çñ=qéãééê~íìêé=~åç=qéãééê~íìêé=pí~äáäáíó=çñ= = Mêçíé~mentioning

confidence: 65%

“…In the pH range 8~10.5, the protease activities were more than 90% of its original and then decreased with increasing pH. Investigation on pH stability of crude enzyme extracts of tuna viscera were in the range of 9~11 with more than 90% activity [24]. The instability of acidic proteases towards the alkaline pH region contrasts with the behaviour of gastric protease of many of the lower vertebrates species [32].…”

Section: Bññéåí=çñ=qéãééê~íìêé=~åç=qéãééê~íìêé=pí~äáäáíó=çñ= = Mêçíé~mentioning

confidence: 99%

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Comparative study of digestive enzymes of squid (Todarodes pacificus) viscera after supercritical carbon dioxide and organic solvent extraction

Uddin

Ahn

Kishimura

et al. 2009

Biotechnol Bioproc E

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^Äëíê~Åí= Three major classes of digestive enzymes of squid viscera were characterized following extraction of oil by supercritical carbon dioxide (SCO 2 ) and organic solvent, n-hexane. Squid viscera were extracted at temperature, 35~45°C and pressure, 15~25 MPa for 2.5 h by SCO 2 with a constant flow rate of 22 g/min. Oil extraction yield increased with the increasing of extraction pressure and temperature. The highest oil extracted residues of squid viscera were used for characterization of digestive enzymes. The activities of protease, lipase, and amylase were highest in n-hexane treated squid viscera samples and lowest in SCO 2 treated samples. The crude extracts of SCO 2 and n-hexane treated squid viscera samples showed almost same optimum pH and pH stability for each of the digestive enzymes. The optimum temperature of protease, lipase, and amylase were found to almost similar in SCO 2 and n-hexane treated samples. But the thermal stability for each digestive enzyme in SCO 2 treated squid viscera were slightly higher than that of n-hexane treated squid viscera. Studies using SDS-PAGE showed no significant differences in protein patterns of the crude extracts of untreated and SCO 2 and n-hexane treated squid viscera indicating no denaturation of proteins. © KSBB hÉóïçêÇëW=ÇáÖÉëíáîÉ=ÉåòóãÉëI=ëèìáÇ=îáëÅÉê~I=ëìéÉêÅêáíáÅ~ä=Å~êÄçå=ÇáçñáÇÉI=åJÜÉñ~åÉI=ÉåòóãÉ=~Åíáîáíó= = = = = fkqolar`qflk= Enzymes have been used as processing aids in various food related industries for a long time [1,2]. In general, enzyme technology has evolved to become an integral part of the food industry. Enzymes have also many other industrial applications. Proteases execute a large variety of functions and have important biotechnological applications. Proteases represent one of the three largest groups of industrial enzymes and find application in detergents, leather industry, pharmaceutical industry, and bioremediation processes [3]. Lipolytic enzymes are currently attracting an enormous attention because of their biotechnological potential. They constitute the most important group of biocatalysts for biotechnological applications. Some of the industrially important chemicals manufactured from fats and oils by chemical G`çêêÉëéçåÇáåÖ=~ìíÜçê=

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Section: Bññéåí=çñ=qéãééê~íìêé=~åç=qéãééê~íìêé=pí~äáäáíó=çñ= = Mêçíé~mentioning

confidence: 65%

Section: Bññéåí=çñ=qéãééê~íìêé=~åç=qéãééê~íìêé=pí~äáäáíó=çñ= = Mêçíé~mentioning

confidence: 99%

Comparative study of digestive enzymes of squid (Todarodes pacificus) viscera after supercritical carbon dioxide and organic solvent extraction

Uddin

Ahn

Kishimura

et al. 2009

Biotechnol Bioproc E

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“…From water soluble extract of mackerel, protease activities were found more than 86 % of its original value, in the range of pH 7.0~8.5 and then decreased with increasing pH. The pH stability of water soluble extracts of tuna and squid viscera were reported to be in the range of 9.0~11.0 and 8.0~10.5, where more than 90 % activity was retained (Parsertsan et al 2001;Uddin et al 2009). Trypsin stability of mackerel extract was in alkaline pH range of 8.0~10.0, above 85 % activity were retained of that pH.…”

Section: Resultsmentioning

confidence: 99%

Characterization of digestive enzymes from de-oiled mackerel (Scomber japonicus) muscle obtained by supercritical carbon dioxide and n-hexane extraction as a comparative study

Asaduzzaman

Chun

2014

J Food Sci Technol

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The oil in mackerel muscle was extracted using an environmental friendly solvent, supercritical carbon dioxide (SC-CO2) at a semi-batch flow extraction process and an n-hexane. The SC-CO2 was carried out at temperature 45 °C and pressures ranging from 15 to 25 MPa. The flow rate of CO2 (27 g/min) was constant at the entire extraction period of 2 h. The highest oil extracted residues after SC-CO2 extraction was used for activity measurement of digestive enzymes. Four digestive enzymes were found in water soluble extracts after n-hexane and SC-CO2 treated samples. Amylase, lipase and trypsin activities were higher in water soluble extracts after SC-CO2 treated samples except protease. Among the four digestive enzymes, the activity of amylase was highest and the value was 44.57 uM/min/mg of protein. The water soluble extracts of SC-CO2 and n-hexane treated mackerel samples showed same alkaline optimum pH and pH stability for each of the digestive enzymes. Optimum temperature of amylase, lipase, protease and trypsin was 40, 50, 60 and 30 °C, respectively of both extracts. More than 80 % temperature stability of amylase, lipase, protease and trypsin were retained at mentioned optimum temperature in water soluble extracts of both treated samples. Based on protein patterns, prominent protein band showed in water soluble extracts after SC-CO2 treated samples indicates no denaturation of protein than untreated and n-hexane.

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“…A study of fish digestive enzyme activity is vital to build and formulate a new feeding system, as well as answering various issues concerning nutritional physiology [10,11,12]. Several studies on digestive enzymes have been investigated in different species such as proteolytic and amylase activities in Oncohinchus mykiss, Sparus aurata, Anguilla Anguilla, Cyprinus carpio, Carassius auratus, and Tinca tinca [9]; proteolytic enzyme in Solea senegalensis [13]; trypsin, amylase, and lipase in prickleback fishes [7]; protease enzymes and free amino acid in Salmo salar [14], proteolytic activity and properties of proteins in S. salar [15], lipase activity in cichlids fishes [16] and protease and lipase enzyme in yellowfin tuna, skipjack tuna and tonggol tuna [17]. However, there is still rare or limited information about grouper digestive enzymes.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary protein and lipid levels on growth performances and digestive enzymes activities in grouper (Epinephelus coioides)

Putra Abdul Samad,

Syahrial,

Wira Hadinata

et al. 2023

BIO Web Conf.

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This study investigates the effect of dietary protein and lipid levels on growth and digestive enzymes activities in grouper (Epinephelus coioides). Furthermore, grouper juveniles (8.63±0.47 g) were separated into ten groups and cultivated in a 100-L tank. Five groups were fed in different protein levels of 35, 40, 45, 50, and 55% protein, while the remaining were fed in different lipid levels of 6, 8, 10, 12, and 14% lipid. All experimental diets were mixed with 1.0% Sauropus androgynus extract. The fishes were sampled for digestive enzyme activities at 1, 4, and 7 days. The results showed that fish receiving 55% protein affected the growth and increased the activities of protease, lipase, carboxypeptidase A (CPA), and carboxypeptidase B (CPB). Liver tissue included higher levels of lipase, as well as carboxypeptidase A and B, while digestive tract tissue contained measurable levels of protease. In conclusion, the administration of grouper juveniles with S. androgynus extract can affect growth performances and increase digestive enzyme activities.

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Production of Enzyme and Protein Hydrolysate from Fish Processing Waste

Cited by 4 publications

References 11 publications

Comparative study of digestive enzymes of squid (Todarodes pacificus) viscera after supercritical carbon dioxide and organic solvent extraction

Comparative study of digestive enzymes of squid (Todarodes pacificus) viscera after supercritical carbon dioxide and organic solvent extraction

Characterization of digestive enzymes from de-oiled mackerel (Scomber japonicus) muscle obtained by supercritical carbon dioxide and n-hexane extraction as a comparative study

Effect of dietary protein and lipid levels on growth performances and digestive enzymes activities in grouper (Epinephelus coioides)

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