Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture

Fernández, Amaury Martín Arzate; Nakazaki, Tetsuya; Yamagata, Hirotada; Tanisaka, Takatoshi

doi:10.1016/s0168-9452(96)04573-6

Cited by 26 publications

(13 citation statements)

References 20 publications

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“…Moreover, the result of our protocol was better than haploid plantlet recovery in Lilium longiflorum Thunb. (Arzate-Fernández et al 1997), Helianthus annuus L (Saji and Sujatha 1998), and Dianthus cinensis L. (Fu et al 2008). In Spatiphyllum wallisii (Araceae), few haploid plants could be successfully regenerated.…”

Section: Discussionmentioning

confidence: 99%

“…The formulae of several basal media such as Chu (N 6 ) (Chu et al 1975), Gamborg's B5 (Gamborg et al 1968); Lichter (NLN) (Huang and Keller 1989); Murashige and Skoog (MS) (Murashige and Skoog 1962), Murashige and Tucker (MT) (Murashige and Tucker 1969), and Nitsch and Nitsch (NN) (Nitsch and Nitsch 1969), among others, were successfully established not only for the tissue culture of various plants and explants, and research objectives (George et al 2007), but also for developing plant anther cultures (Arzate-Fernández et al 1997;Ishizaka 1998;Nomizu et al 2004;Seguí-Simarro and Nuez 2007;Cao et al 2010;Doi et al 2010;Sayem et al 2010), the most commonly used being the MS medium formulation (Thengane et al 1994;Han et al 1997;Saji and Sujatha 1998;Metwally et al 1998;Rimberia et al 2006;Wang and Bao 2007;Fu et al 2008;Sayem et al 2010). This medium was previously developed for optimal growth of tobacco callus but is now applied in many plants by adapting the strength of macro-nutrients, or minor changes in the concentrations of minerals (Bouman and Tiekstra 2001).…”

Section: Introductionmentioning

confidence: 99%

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New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Winarto¹,

Rachmawati²,

Silva

2011

Plant Growth Regul

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A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0 mg/l), a-naphthalene acetic acid (0.01-0.2 mg/l), thidiazuron (0.5-2.0 mg/l), 6-benzylaminopurine (0.5-1.0 mg/l), and kinetin (0.5-1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14-18), 15 aneuploid (n = 20-26), 126 diploid (n = 28-34) and 5 triploid (n = 45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Winarto¹,

Rachmawati²,

Silva

2011

Plant Growth Regul

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“…cv. 'Wase Teppo Yuri' and the Asiatic hybrid lily 'Connecticut King' (Arzate-Fernández et al 1997;Han et al 1997), an interspecific F 1 hybrid between Cyclamen persicum and C. purpurascens (Ishizaka 1998), Helianthus annuus L. 'Morden' (Thengane et al 1994;Saji and Sujatha 1998), and Dianthus chinensis L. 'Carpet' (Fu et al 2008). In the Araceae, especially in Anthurium, the use of anther culture is still very limited.…”

Section: Introductionmentioning

confidence: 99%

“…In ornamental plants, variation among plants derived from anther cultures was recorded in Lilium longiflorum (Qu et al 1988;Arzate-Fernández et al 1997), Asiatic hybrid lily (Han et al 1997), Helianthus annuus L. 'Morden' (Thengane et al 1994;Saji and Sujatha 1998), Dianthus chinensis L. (Fu et al 2008), and Gentiana triflora (Doi et al 2010). In other horticultural plants, variation was reported in Daucus carota (Kozik et al 2002), Citrus clementina Hort.…”

Section: Introductionmentioning

confidence: 99%

“…Aneuploidy, haploidy, diploidy, triploidy, tetraploidy, octoploidy, mixoploidy, etc. were cytological alterations commonly derived from anther culture (Qu et al 1988;Thengane et al 1994;Arzate-Fernández et al 1997;Han et al 1997;Ockendon 1998;Saji and Sujatha 1998;Chiancone et al 2006;Rimberia et al 2006;Kowalska et al 2008;Doi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

Winarto¹,

Rachmawati²,

Pramanik³

et al. 2010

Plant Cell Tiss Organ Cult

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Anthurium anther culture was successfully established using half-anthers as explants. Explants were cultured on Winarto-Teixeira basal medium (WT-1) containing 0.01 mg/l a-naphthalene acetic acid (NAA), 0.5 mg/l thidiazuron (TDZ), and 1.0 mg/l 6-benzylaminopurine (BAP), or on New Winarto-Teixeira basal medium (NWT-3) supplemented with 0.02 mg/l NAA, 1.5 mg/l TDZ, and 0.75 mg/l BAP for callus initiation. Regenerated calli produced multiple shoots on WT-1, which were then rooted in NWT-3 supplemented with 1% activated charcoal. Plantlets were acclimatized ex vitro using a mixture of burned rice husk, rice husk, and bamboo peat (1:1:1, v/v/v) as the potting medium. There was considerable morphological and cytological diversity of regenerants derived from anther culture, which are described in detail in this study. The callus cluster color ranged from green to light green and had a high regeneration capacity (7.3 and 4.8 shoots/callus cluster), light reddish-yellow callus showed moderate regeneration (2.6 shoots/callus cluster), while reddish-yellow callus had the lowest regeneration capacity (1.5 shoots/callus cluster). Morphological variations clearly observed in regenerants derived from this technique included alterations in plant size, peduncle length, spathe position compared to leaves, the type and number of buds, spathe and spadix color, and spadix length. There were also cytological variations in both in vitro and ex vitro regenerants of anther culture with 23-29% haploids, 5-10% aneuploids, 56-69% diploids, and 3-4% triploids. The results strengthen other studies in which the development of anther cultures, especially via callus formation, resulted in morphological and cytological alterations. These variations have been discussed to great length in this paper.

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Species with Haploid or Doubled Haploid Protocols

Seguí-Simarro

Moreno

Fernández

et al. 2021

Methods in Molecular Biology

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Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture

Cited by 26 publications

References 20 publications

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

Species with Haploid or Doubled Haploid Protocols

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