Production of doubled haploid lines in frequencies sufficient for barley breeding programs

Kuhlmann, Ursula; Foroughi‐Wehr, B.

doi:10.1007/bf00716843

Cited by 90 publications

(38 citation statements)

References 4 publications

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“…In cereal crop somatic tissue culture, sucrose is most frequently used in culture media for somatic embryogenesis. Maltose has been shown to be the most effective sugar for increasing androgenesis and green plant regeneration in rye (Flehinghaus et al 1991), wheat (Moieni 1997) and barley (Kuhlmann and Foroughi-Wehr 1989, However, our results revealed maltose (3%) to be an inferior carbohydrate source in comparison with sucrose (3%) for somatic embryogenesis of rye.…”

Section: Sugar and Gelling Agent In Culture Mediumcontrasting

confidence: 64%

Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

Ma¹,

Pulli²

2008

AFSci

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Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a total 27 rye genotypes including 10 spring and 17 winter genotypes were involved in the investigation. Genotype, culture medium, sugar, gel agent and auxin infl uenced somatic embryogenesis of immature embryo signifi cantly. One-two weeks cold pretreatment of young embryo enhanced somatic embryogenesis and green plant regeneration. In culture of immature embryos, infl orescences and leaf segments of the seedlings, explants signifi cantly infl uenced the culture effi ciency. Highest embryogenic callus yield resulted from rye immature embryo as explant compared to young infl orescence and leaf segment of seedling. Developmental stage of embryo played an important role in somatic embryogenesis. Late spherical coleoptile stage (embryo size 0.5-1mm in length) was optimal developmental stage of immature embryo for culture. Morphogenetic potential of embryogenic callus decreased with an increasing number of subcultures, and this ability could be maintained in vitro for a maximum of 8 months of culturing.

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Section: Sugar and Gelling Agent In Culture Mediumcontrasting

confidence: 64%

Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

Ma¹,

Pulli²

2008

AFSci

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“…P reliminary e xperiments in camelina using basal media compositions commonly used in androgenesis indicated that there was no response from two formulations evaluated, i.e. N6 (Chu 1978) and KFWC (Kuhlmann and Foroughi-Wehr 1989). There was minimal response from NN (Nitsch and Nitsch 1969) (data not shown).…”

Section: Resultsmentioning

confidence: 94%

A microspore embryogenesis protocol for Camelina sativa, a multi-use crop

Ferrie

Bethune

2011

Plant Cell Tiss Organ Cult

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Camelina [Camelina sativa (L.) Crantz], a member of the Brassicaceae family, has a unique oil profile that has potential both for biofuels and as a food crop. It is essential to have a doubled haploidy protocol in order to enhance breeding of this crop for prairie conditions as well as improve the yield and quality characteristics, Microsporederived embryos have been produced from Camelina sativa. Buds 1 -3 mm in length were selected for culture.The microspores were isolated and purified in full-strength B5 extraction medium and cultured in NLN medium with 12.5% sucrose and 12.5% polyethylene glycol 4000 (PEG) without glutamine, at a density of 10,000 microspores per mL. Glutamine was added to the cultures 72 h after extraction to give a final concentration of 0.8 g/L. The microspore cultures were maintained at 24°C in the dark. After 28 days embryos were observed and these were regenerated to plants and selfed seed was produced. The highest embryogenic frequency achieved was 38 microspore-derived embryos from 100,000 microspores.

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“…As described in a previous paper (Stival da Silva et al, 1997) this method has been used to produce an average of 3,5 green plants/100 plated anthers. According to Foroughi-Wehr & Friedt (1984) and Kühlmann & Foroughi-Wehr (1989) this rate of doubled haploid production enables the technique to be applied in barley breeding programs.…”

Section: Resultsmentioning

confidence: 99%

Ontogenetic events in androgenesis of Brazilian barley genotypes

2000

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This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.Key words: anther culture, doubled haploids. RESUMO Método para obtenção de duplo-haplóides de genótipos brasileiros de cevadaAnteras de cevada (Hordeum vulgare L.) foram cultivadas in vitro, fazendo com que houvesse uma reversão no desenvolvimento dos micrósporos para um modo esporofítico de desenvolvimento. Uma primeira mitose simétrica, seguida de uma seqüência de mitoses, levou à formação de micrósporos multinucleados. A regeneração de plantas ocorreu tanto por embriogênese direta quanto pela formação de calos, com posterior diferenciação via embriogênese indireta ou organogênese. Os embriões e calos androgenéticos mostraram grande potencial para regeneração, e plantas verdes e albinas foram regeneradas no mesmo meio utilizado para indução, sendo a transferência para outro meio necessária somente para dar prosseguimento ao desenvolvimento e ao enraizamento das plântulas. A possibilidade de induzir pró-embrióides e regenerar plantas em um único passo, sem necessidade de transferência para um meio específico de regeneração, aumenta a rapidez e a eficiência da técnica de cultura de anteras.Palavras-chave: cultura de anteras, duplo-haplóides.

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Production of doubled haploid lines in frequencies sufficient for barley breeding programs

Cited by 90 publications

References 4 publications

Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

A microspore embryogenesis protocol for Camelina sativa, a multi-use crop

Ontogenetic events in androgenesis of Brazilian barley genotypes

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