Production of DNA-hydrolyzing Antibody BV04-01 Fab Fragment in Methylotrophic Yeast Pichia pastoris

Kozyr, A. V.; Бобик, Т. В.; Ignatova, A. N.; Kolesnikov, Alexander V.

doi:10.1023/b:mbil.0000049871.17260.46

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…way Ester hydrolysis 41S-2-L Mamm.cell Cleavage of gp41 peptides Rabbits Hifumi et al (2002) [ 81 ] scFv 4B2 ProK–Cytopl. way allylic isomerization and Kemp elimination E. coli Robin et al (2003) [ 108 ] EuK–Yeast P. pastoris EuK–Yeast K. lactis mAb 9A8 Mammalian ascites Acetylcholinesterase Franqueville et al (2003) [ 109 ] Fab BV04-01 EuK–Yeast DNA hydrolysis P. pastoris Kozyr et al (2004) [ 110 ] ECL2B-2 Mamm.cell Cleaving a Chemokine Receptor CCR-5 Peptide Mice Mitsuda et al (2004) [ 84 ] Fab 6D9 Transcription/translation system Ester hydrolysis E. coli cell-free expression system Ali et al (2005) [ 111 ] A.17 ProK Amidase activities E. coli Reshetnyak et al (2007) [ 112 ] Ab2 6B8-E12 ProK Proteolytic activity E. coli Ponomarenko et al (2007) [ 48 ] …”

Section: Other Methods Of Producing Catalytic Antibodiesmentioning

confidence: 99%

“…Because the prokaryotic host lacks the function of protein post-translational modification in eukaryotic cells, which is critical for maintaining the stability of some catalytic antibodies. Furthermore, catalytic antibodies may be harmful to bacterial hosts, an issue that can be efficiently avoided by isolating heterologous proteins from eukaryotic cells [ 62 , 110 ]. In this case, using a yeast expression system to express catalytic antibodies is a good idea [ 121 ].…”

Section: Other Methods Of Producing Catalytic Antibodiesmentioning

confidence: 99%

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Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Zhao

Chen

et al. 2022

Appl Biochem Biotechnol

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Catalytic antibodies made it feasible to develop new catalysts, which had previously been the subject of research. Scientists have discovered natural antibodies that can hydrolyze substrates such as nucleic acids, proteins, and polysaccharides during decades of research, as well as several ways of producing antibodies with specialized characteristics and catalytic functions. These antibodies are widely used in chemistry, biology, and medicine. Catalytic antibodies can continue to play a role and even fully prevent the emergence of autoimmune disorders, especially in the field of infection and immunity, where the process of its occurrence and development often takes a long time. In this work, the development, design and evolution methodologies, and the expression systems and applications of catalytic antibodies, are discussed. Trial registration: not applicable.

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Section: Other Methods Of Producing Catalytic Antibodiesmentioning

confidence: 99%

Section: Other Methods Of Producing Catalytic Antibodiesmentioning

confidence: 99%

Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Zhao

Chen

et al. 2022

Appl Biochem Biotechnol

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Catalytic antibodies and their applications in biotechnology: state of the art

et al. 2014

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Catalytic antibodies are immunoglobulins endowed with enzymatic properties. Discovered in the second part of the 1980s, the enthusiasm they initially aroused was counterbalanced by the difficulty of their production and their low catalytic rates. Nevertheless, improvements in expression systems and engineering technologies, combined with various studies suggesting that catalytic antibodies play a role in the immune system, have opened the way to new applications for these proteins. Herein we review catalytic antibodies from a biotechnological point of view, focusing our study on the different production methods, expression systems and their potential clinical applications dedicated to these proteins.

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Ribosomal tunnel and translation regulation

Bøgdanov

Sumbatyan

Shishkina

et al. 2010

Biochemistry Moscow

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This review describes the results of recent studies of the ribosomal tunnel (RT), the major function of which is to allow the smooth passage of nascent polypeptides with different sequences from the peptidyl transferase center of the ribosome to the tunnel exit, where the folding of protein molecules begins. The features of structural organization of RT and their role in modulation and stabilization of the nascent chain conformation are discussed. Structural features of macrolide binding sites as well as application of macrolide antibiotics and their derivatives as tools to investigate ligand-tunnel wall interactions are also considered. Several examples of strong and specific interactions of regulatory polypeptides with nucleotide and amino acid residues of RT that lead to ribosome stalling and translational arrest are described in detail. The role of these events in regulation of expression of certain genes is discussed on the basis of recent high-resolution structural studies of nascent chains in the RT.

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Production of DNA-hydrolyzing Antibody BV04-01 Fab Fragment in Methylotrophic Yeast Pichia pastoris

Cited by 4 publications

References 34 publications

Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Catalytic antibodies and their applications in biotechnology: state of the art

Ribosomal tunnel and translation regulation

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