Production of d-β-hydroxyisobutyric acid from isobutyric acid by Candida rugosa

Lee, In Young; Hong, Won Kyoung; Hwang, Young Bo; Kim, Chul Ho; Choi, Eui Sung; Rhee, Shin Hyung; Park, Young Hoon

doi:10.1016/0922-338x(96)83126-6

Cited by 6 publications

(9 citation statements)

References 9 publications

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“…It was also noted that, however, methacrylic acid (MA) has potential as a substrate for D-HIBA production since methacrylyl-CoA is a precursor metabolite in the D-HIBA synthetic pathway [6]. Recently, we reported continuous production of D-HIBA using MA under ammonium-limited conditions [7]. The productivity of D-HIBA was as high as 0.86 g/l/h.…”

Section: Introductionmentioning

confidence: 94%

High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant

Lee

Kim

Yeon

et al. 1997

Bioprocess Engineering

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Production of D--hydroxyisobutyric acid (D-HIBA) from methacrylic acid (MA) was investigated using Candida rugosa IFO 0750 and its mutant. Cell growth decreased as the MA concentration increased and was inhibited at D-HIBA concentrations higher than 30 g/l. Optimal MA concentration for D-HIBA production was in the range of 10-20 g/l. It was also noted that cell growth and D-HIBA production were inhibited by higher concentrations of Na + , K + , and NH 4 + , which were required for pH control during cultivation. With a suitably designed feeding mode of MA, the parent strain produced 65 g/l of D-HIBA after 120 h of fed-batch cultivation, but molar conversion yield of D-HIBA was less than 40%. The mutant, unable to assimilate propionic acid, produced as high as 70 g/l of D-HIBA in the same culture period with a molar conversion yield of more than 70%.

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Section: Introductionmentioning

confidence: 94%

High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant

Lee

Kim

Yeon

et al. 1997

Bioprocess Engineering

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“…The production rate of β-hydroxyisobutyric acid increased as the glucose concentration decreased, while the conversion yield of isobutyric acid to β-hydroxyisobutyric acid showed an opposite trend. With controlled feeding of isobutyric acid and glucose, a high titer of β-hydroxyisobutyric acid was obtained by a fed-batch cultivation of the producing strain [82,83]. …”

Section: Production Of Optically Active Compoundsmentioning

confidence: 99%

“…Candida rugosa IFO 0750 is the effective biocatalyst for the oxidation of isobutyric acid to ( R )-β-hydroxyisobutyric acid [81,82]. Under optimal conditions the product was obtained with a concentration of 100 g/L (productivity 0.83 g/L/h), but the molar conversion yield of the product did not exceed 40% [82].…”

Section: Production Of Optically Active Compoundsmentioning

confidence: 99%

Biotransformations Utilizing β-Oxidation Cycle Reactions in the Synthesis of Natural Compounds and Medicines

Œwizdor

Panek

Milecka-Tronina

et al. 2012

IJMS

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β-Oxidation cycle reactions, which are key stages in the metabolism of fatty acids in eucaryotic cells and in processes with a significant role in the degradation of acids used by microbes as a carbon source, have also found application in biotransformations. One of the major advantages of biotransformations based on the β-oxidation cycle is the possibility to transform a substrate in a series of reactions catalyzed by a number of enzymes. It allows the use of sterols as a substrate base in the production of natural steroid compounds and their analogues. This route also leads to biologically active compounds of therapeutic significance. Transformations of natural substrates via β-oxidation are the core part of the synthetic routes of natural flavors used as food additives. Stereoselectivity of the enzymes catalyzing the stages of dehydrogenation and addition of a water molecule to the double bond also finds application in the synthesis of chiral biologically active compounds, including medicines. Recent advances in genetic, metabolic engineering, methods for the enhancement of bioprocess productivity and the selectivity of target reactions are also described.

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“…Many other organisms are also capable of this oxidation, e.g., immobilized Bullera alba I F 0 1030 which gives higher yields than using free cells (KANDA et al, 1993). Alternatively, (R)-( -)-phydroxy-isobutyric acid is accessible by hydroxylation of isobutyric acid using Candida rugosa (LEE et al, 1996;HOLLAND, 1992a).…”

Section: Genetically Engineered Organismsmentioning

confidence: 99%

Hydroxylation and Dihydroxylation

Holland¹

2001

Biotechnology Set

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Production of d-β-hydroxyisobutyric acid from isobutyric acid by Candida rugosa

Cited by 6 publications

References 9 publications

High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant

High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant

Biotransformations Utilizing β-Oxidation Cycle Reactions in the Synthesis of Natural Compounds and Medicines

Hydroxylation and Dihydroxylation

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