Production of culture filtrates of Sporothrix schenckii in diverse culture media

Mendoza, Mireya; Díaz, A. M.; Hung, M. B.; Zambrano, E. A.; Díaz, E.; Albornoz, María B. de

doi:10.1080/714031144

Cited by 12 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although in recent years we have seen noticeable advances in the specific immune diagnosis of many human deep mycoses, it has not been the case for human sporotrichosis 4,6,8,21 .…”

Section: Discussionmentioning

confidence: 99%

“…Contrary to other studies that have used mycelial phase or in the best of conditions mixed cultures of mycelia, conidia and yeasts with unknown viability 21,30 ; we have developed a system to quantify the percentage of yeast phase in any passage until we could label the culture pure in one of the species. In order to label the culture as pure yeast phase or 100% yeast phase we had to ascertain after examination in a hemocytometer that there were yeasts in all fields, we counted at least 500 cells and we could not see any conidia or mycelia.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Excretion-secretion products and proteases from live Sporothrix schenckii yeast phase: immunological detection and cleavage of human IgG

Rosa

Gezuele

Calegari

et al. 2009

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

suMMARYAntigenic preparations from Sporothrix schenckii usually involve materials from mixed cultures of yeast and mycelia presenting cross-reactions with other deep mycoses. We have standardized pure yeast phase with high viability of the cells suitable to obtain specific excretion-secretion products without somatic contaminations. These excretion-secretion products were highly immunogenic and did not produce noticeable cross-reactions in either double immunodiffusion or Western blot. The antigenic preparation consists mainly of proteins with molecular weights between 40 and 70 kDa, some of them with proteolytic activity in mild acidic conditions. We also observed cathepsin-like activity at two days of culture and chymotrypsin-like activity at four days of culture consistent with the change in concentration of different secreted proteins. The proteases were able to cleave different subclasses of human IgG suggesting a sequential production of antigens and molecules that could interact and interfere with the immune response of the host.

show abstract

“…Although in recent years we have seen noticeable advances in the specific immune diagnosis of many human deep mycoses, it has not been the case for human sporotrichosis 4,6,8,21 .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Excretion-secretion products and proteases from live Sporothrix schenckii yeast phase: immunological detection and cleavage of human IgG

Rosa

Gezuele

Calegari

et al. 2009

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…Some studies have investigated the protein secretion profile of Sporothrix, 43,44 the genetic variability, 11,12,41,42 as well as virulence of some new species. 2,3 This is the first study which evaluates the protein secretion of S. brasiliensis, S. globosa and S. schenckii s. str.…”

Section: 124142mentioning

confidence: 99%

Characterization of virulence profile, protein secretion and immunogenicity of differentSporothrix schenckii sensu strictoisolates compared withS. globosaandS. brasiliensisspecies

et al. 2013

View full text Add to dashboard Cite

“…The optimum expression of these principal antigenic components of S. schenckii occurs in the stationary phase of cultures grown in Sabouraud dextrose broth (22). Accordingly, we describe here the application of mycelial S. schenckii exoantigens in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody responses in patients with sporotrichosis.…”

mentioning

confidence: 99%

Use of Mycelial-Phase Sporothrix schenckii Exoantigens in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Sporotrichosis by Antibody Detection

Almeida‐Paes

Pimenta

Pizzini

et al. 2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

An enzyme-linked immunosorbent assay (ELISA) was developed for specific antibody detection in serum specimens of patients with sporotrichosis. The assay was made with mycelial-phase Sporothrix schenckii exoantigens and was tested against 90 sera from patients with different clinical forms of sporotrichosis. Potential cross-reactions were analyzed with 72 heterologous sera from patients with paracoccidioidomycosis, cryptococcosis, aspergillosis, histoplasmosis, tuberculosis, and American tegumentary leishmaniasis, as well as 76 sera from healthy controls. We found a sensitivity of 97% and a specificity of 89% in this assay. Some cross-reactions were seen, as observed in other immunoassays for the diagnosis of sporotrichosis. The ELISA appears to be especially useful for cutaneous forms of disease, since these are not promptly diagnosed with available immunoprecipitation or agglutination techniques. These results suggest that the ELISA using mycelial-phase S. schenckii exoantigens is a very sensitive diagnostic tool for the serodiagnosis of sporotrichosis and can be used in conjunction with conventional methods of diagnosis, particularly in cases where crossreactions or false-positive results are experienced with the serodiagnosis.

show abstract

Production of culture filtrates of Sporothrix schenckii in diverse culture media

Cited by 12 publications

References 0 publications

Excretion-secretion products and proteases from live Sporothrix schenckii yeast phase: immunological detection and cleavage of human IgG

Excretion-secretion products and proteases from live Sporothrix schenckii yeast phase: immunological detection and cleavage of human IgG

Characterization of virulence profile, protein secretion and immunogenicity of differentSporothrix schenckii sensu strictoisolates compared withS. globosaandS. brasiliensisspecies

Use of Mycelial-Phase Sporothrix schenckii Exoantigens in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Sporotrichosis by Antibody Detection

Contact Info

Product

Resources

About