Production of Conjugated Linoleic Acid (CLA) by Bifidobacterium breve LMC520 and Its Compatibility with CLA-Producing Rumen Bacteria

Abstract

This study was performed to characterize the ability of an active Bifidobacterium strain to produce conjugated linoleic acid (CLA) and to test its possible utilization as a probiotic compatible to the ruminal condition. Bifidobacterium breve LMC520 can actively convert linoleic acid (LA) to cis-9,trans-11-CLA, which is a major isomer derived from microbial conversion. LMC520 showed reasonable tolerance under acidic conditions (pH 2.5 with 1% pepsin) and in the presence of oxgall (0-3%). The growth and CLA production of LMC520 were tested under ruminal conditions and compared with those of Butyrivibrio fibrisolvens A38, which is a major CLA producer in the rumen as an intermediate in the biohydrogenation (BH) process. LMC520 converted 15% of LA to CLA under ruminal conditions, which was 2 times higher activity than that of A38, and there was no decline in CLA level during prolonged incubation of 48 h. The BH activity of LMC520 was comparable to that of A38. When LMC520 was cocultured with A38, even with slight decrease of CLA due to high BH activity by A38, but the level of CLA was maintained by the high CLA-producing activity of LMC520. This comparative study shows the potential of this strain to be applied as a functional probiotic not only for humans but also for ruminants as well as to increase CLA production.

Production of a Conjugated Fatty Acid by Bifidobacterium breve LMC520 from α-Linolenic Acid: Conjugated Linolenic Acid (CLnA)

“…In addition, B. breve LMC520 showed compatibility with CLA-producing rumen bacteria in terms of the growth and fat substrate conversion, which indicated that this bacterium could be useful as a functional probiotics for ruminant use. 23 Overall, the conversion of α-LNA to CLnA by B. breve LMC 520 was dependent upon certain environmental factors, including incubation time, substrate concentration, pH, and atmospheric conditions. B. breve LMC520 was more active in CLnA production as compared to CLA production.…”

Recent Development of Probiotic Bifidobacteria for Treating Human Diseases

“…Many Bifidobacteria are natural producers of conjugated linoleic acids (CLA), resulting from the biohydrogenation of linoleic acid (LA) and other unsaturated fatty acids ( Figure 2 ). The first reported strain that produces CLA is B. breve with a 66% endpoint conversion, where the converted LA to CLA was secreted into the surrounding medium ( Park et al, 2011 ). Other strains showing a similar ability to convert LA to CLA includes B. infantis, B. dentium, B. catenulatum and B. pseudocatenatalum ( Raimondi et al, 2016 ) .…”

Microbial ecology of the rumen evaluated by 454 GS FLX pyrosequencing is affected by starch and oil supplementation of diets

“…The addition of starch increased the abundance of Bifidobacterium and Olsenella genera but decreased that of Atopobium genus. Information about these genera is not commonly found in the literature except Bifidobacterium genus which seems to be implicated in the production of conjugated linoleic and conjugated linolenic acids isomers (Gorissen et al, 2010;Park et al, 2011). Bifidobacterium spp.…”