Production of Common Sour Orange × Carrizo Citrange Hybrids Using Embryo Rescue

Kurt, Şenay; Ülger, Salih

doi:10.1080/15538362.2013.801729

Cited by 11 publications

(13 citation statements)

References 15 publications

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“…Earlier, Wang et al (1999) reported in vitro embryo germinations after 50 days in different cross-combinations of citrus, while Tan et al (2007) found embryo germination after 80 days in mandarin × trifoliate. Furthermore, variation in in vitro embryo germination due to harvesting stage was also reported in pomelo and tangerine (Deng et al 1996), 'Femminello' (lemon cybrids) × 'Femminello' lemon hybrids (Scarano et al 2003), the hybrid of Cleopatra mandarin × Carrizo citrange and Cleopatra mandarin × flying dragon (Turgutoglu et al 2015) and sweet orange (Das et al 2000;Kurt & Ulger 2014). In vitro embryo germination influenced by the composition of medium and embryo age has also been previously reported in oil palm (Pádua et al 2014), sweet orange, and mandarin (Chagas et al 2005) and mandarin × trifoliate orange hybrid (Tan et al 2007).…”

Section: Fruit Set Functional Embryos and Embryo Germinationmentioning

confidence: 85%

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Dubey

Gupta

Sharma

et al. 2020

Journal of Horticultural Research

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Nucellar embryony is the major obstacle in getting hybrid seedlings in interspecific crosses in citrus. Hence, the present study was conducted to standardize embryo age, culture media for maximizing germination, and subsequent identification of simple sequence repeat (SSR) markers to differentiate the hybrids. A factorial experiment was conducted with three embryo ages – 80–90, 110–120, and 130–140 days after pollination (DAP). The germination of the rescued immature embryos of acid lime × lemon crosses was initiated on three different growing media. The fruits harvested at 130–140 and 80–90 DAP had a higher number of functional seeds (90%). Older embryos (130–140 DAP) and middle-aged embryos (110–120 DAP) germinated in 100% on Murashige and Skoog (MS) medium supplemented with 500 mg·l−1 malt extract, 1.5 mg·l−1 gibberellic acid (GA3), and 0.02 mg·l−1 naphthalene acetic acid (NAA). MS medium supplemented with 500 mg·l−1 malt extract proved better for the germination of embryos taken at 80–90 DAP. Plantlet survival was the highest in younger embryos (80–90 DAP) cultured on MS basal medium (84.21%) and the lowest in older embryos cultured on MS medium supplemented with 500 mg·l−1 malt extract plus 1.5 mg·l−1 GA3 and 0.02 mg·l−1 NAA. The seedlings obtained from the culture 80–90 DAP had the highest root length (4.9 mm) and shoot length (5.3 mm) at 60 days after inoculation (DAI) on the above medium. SSR marker analysis revealed that CCSM-4 and CAC-33 markers expressed polymorphism between female and male parents, proving their ability to identify the hybrids of ‘Kagzi’ acid lime × ‘Konkan Seedless’ lemon.

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Section: Fruit Set Functional Embryos and Embryo Germinationmentioning

confidence: 85%

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Dubey

Gupta

Sharma

et al. 2020

Journal of Horticultural Research

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“…In general, older embryos (120–130 DAP) had higher germination on either of the medium, contrasting younger embryos proved better while cultured on M1 medium in respect of plantlet survival at 30 DAI, though this medium was also found effective to maintain higher plantlet survivability of old aged embryos too. Previously researchers have reported in vitro embryo germinations after 50 days (Wang et al, ), 80 days (Tan, Song, & Deng, ), 100 days (Deng, Yi, Li, & Guo, ), 105 days (Scarano et al, ), 115 days (Turgutoglu, Kurt, & Demir, ) and 120 days (Das, Paul, & Chaudhuris, ; Kurt & Ulger, ). Among media, older embryos germinated well at a higher rate on M1 medium, while it was M2 medium which converted highest embryos into plantlets.…”

Section: Discussionmentioning

confidence: 98%

“…Initially, white medium (White, ) was used as basic medium for tissue culture in several crop species, but subsequent alterations in its composition have given rise to the development of MS medium (Murashige & Skoog, ), Gamborg B5 medium (Gamborg, Miller, & Ojima, ), MT medium (Murashige & Tucker, ) and DKW medium (Driver & Kuniyuki, ). In citrus, many researchers have tried to alter the varied factors including pH, Agar, sucrose, malt, GA 3 concentrations (Carimi et al, ; Ribeiro & Walter, ), macro‐and micro‐nutrients, vitamins of the MT medium (Morais, Vilegas, & Lanc, ) and developmental stages (Carimi et al, ; Kurt & Ulger, ). Malt extract appears to play a vital role in embryo cultures of Citrus species (Molnar, Virag, & Ordog, ).…”

Section: Introductionmentioning

confidence: 99%

Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

et al. 2019

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In citrus, development of new hybrids is difficult due to failure of the germination of zygotic embryos. Hence, this study was conducted to standardize embryo age and media for maximizing the germination and subsequent seedling growth in sour orange × Sacaton citrumelo crosses followed by the identification of simple sequence repeats (SSR) markers for distinguishing the hybrids. A factorial experiment in complete randomized design was conducted with two each embryo age (110–120 and 130–140 days after pollination) and culture media (G‐B5 supplemented with 1.5 mg/L GA3 plus malt extract and G‐B5 plus malt extract) for in vitro embryo culture of sour orange × Sacaton citrumelo progenies. Older embryos germinated well in both media, though highest on M1 medium (100%). In fact, M1 medium gave higher plantlet survival in both age groups. The seedling growth of older embryos had highest root length (37.80 mm) and shoot length (24.20 mm) in M1 medium at 60 days after inoculation (DAI). Three SSR markers (TAA45, CAC15 and CAC39) showed polymorphism between female and male parents and were able to identify their hybrids.

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“…Many researchers have reported varied number of days after pollination (DAP) for higher embryo recovery in different citrus species, varying from 80-150 DAP, namely, 80 DAP in mandarin × trifoliate orange [10], 105 DAP in Femminello lemon cybrids (2n and 4n) and a diploid clone of Femminello lemon (Citrus limon L. Burm. F.) [29], 115 DAP in Cleopatra mandarin × Carrizo citrange and Cleopatra mandarin × Flying Dragon [30], 118 DAP in Pera Rio Sweet orange × Ponca mandarin [31], 120 DAP in common sour orange [32], and 135-150 DAP in lemon [28].…”

Section: Number Of Embryos and Plant Recoverymentioning

confidence: 99%

Optimizing Recovery of Hybrid Embryos from Interspecific Citrus Crosses of Polyembryonic Rough Lemon (Citrus jambhiri Lush.)

et al. 2020

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The prevalence of rough lemon rootstock in India is presently threatened by the spread of Phytophthora root rot. Developing an alternative Phytophthora-resistant rootstock that also withstands other abiotic constraints is a priority for a sustainable citrus industry in India. Complementary progenitors can be found in citrus germplasm to combine the desired traits, particularly between Citrus and Poncirus, a closely related genus. The production of hybrids accumulating the desired dominant traits, irrespective of the heterozygosity level of the parents, is an effective way to develop new candidate rootstocks. Rough lemon was hybridized with Swingle citrumelo, X-639 citrandarin, and GouTou Cheng. A reliable method was developed for greater recovery of hybrid seedlings from these crosses on the basis of morphology and simple sequence repeat (SSR) markers. The greatest proportion of hybrid seedlings resulted from embryo rescue at 95 days after pollination (DAP) (48.5% in rough lemon × X-639 citrandarin and 56.7% in rough lemon × Swingle citrumelo) on the basis of multifoliate leaves. The proportion of hybrid seedlings decreased with increase in embryo development stage after 95 DAP until the last stage of observation (125 DAP). Significantly higher proportion of multifoliate seedlings was recovered by in vitro culture compared with in vivo germination from the mature seeds (26.9% vs. 5.2%, respectively). Swingle and X-639 citrandarin are not homozygous for the multifoliate allele from Poncirus, and GouTou Cheng lacks the multifoliate allele; thus, unifoliate hybrids were selected from all crosses on the basis of SSR marker genotypes.

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Production of Common Sour Orange × Carrizo Citrange Hybrids Using Embryo Rescue

Cited by 11 publications

References 15 publications

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Maximizing Hybrid Seedlings Recovery and Early Identification of Highly Polyembryonic Acid Lime (Citrus aurantifolia Swing.) × Lemon (Citrus limon Burm.) Hybrids using SSR Markers

Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange (Citrus aurantium) × Sacaton citrumelo (C. paradisi × Poncirus trifoliata) crosses through embryo rescue

Optimizing Recovery of Hybrid Embryos from Interspecific Citrus Crosses of Polyembryonic Rough Lemon (Citrus jambhiri Lush.)

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