Production of collared peccary (Pecari tajacu Linnaeus, 1758) parthenogenic embryos following different oocyte chemical activation and in vitro maturation conditions

Borges, Alana Azevedo; Santos, Maria Valéria de Oliveira; Nascimento, Lucas Emanuel; Lira, Gabriela Pereira de Oliveira; Praxedes, Érika Almeida; Oliveira, Moacir Franco de; Silva, Alexandre Rodrigues; Pereira, Alexsandra Fernandes

doi:10.1016/j.theriogenology.2019.10.016

Cited by 7 publications

(11 citation statements)

References 45 publications

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“…Regarding the effect of adding EGF, in Iberian red deer, an increase in oocyte maturation rate was observed in a medium supplemented with FSH and LH and 50 ng/mL EGF (46.3%) than in the medium without EGF (19.5%, Macías-García et al, 2018). In collared peccary, we observed that the presence of 10 ng/mL of EGF during IVM did not improve meiotic development, but improved oocyte morphometry (Borges et al, 2020). Thus, different oocyte responses to this growth factor are observed in these ungulates and are likely owing to the physiological characteristics of each species.…”

Section: In Vitro Maturation Conditionsmentioning

confidence: 81%

“…Several in vitro culture protocols of domestic and wild ungulate embryos use SOF medium, although in domestic swine, the medium for porcine embryos (PZM) is widely used. Nevertheless, interestingly, collared peccary embryos showed good development in vitro using SOF medium (Borges et al, 2020).…”

Section: In Vitro Fertilization and Embryo Culturementioning

confidence: 99%

“…Normally, the same base medium has been used in wild ungulates, such as wood bison (Cervantes et al, 2016), gazelles (Berlinguer et al, 2008), mouflon (Ptak et al, 2002), deer species (Berg et al, 2002;Locatelli et al, 2012;Thongphakdee et al, 2017), collared peccary (Pecari tajacu, Borges et al, 2020), and zebra (Meintjes et al, 1997). Interestingly, for black rhinoceros oocytes IVM, Stoops et al (2011) used TCM-199 with 10% fetal bovine serum and obtained only 3.6% (3/83) oocytes in metaphase II (MII).…”

Section: In Vitro Maturation Conditionsmentioning

confidence: 99%

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Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Santos

Silva

Pereira

2022

Annals of Animal Science

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Wild ungulates are of fundamental importance for balancing ecosystems, as well as being the species of economic interest. Increasing concern over the accelerated population reduction of these species has resulted in the development of assisted reproduction techniques, such as in vitro fertilization (IVF), as a tool for conservation and multiplication. In the present scenario, IVF protocols were developed based on the methodologies used for domestic ungulates. Nevertheless, owing to the physiological and reproductive differences among the species, several factors associated with IVF and its relationship with the characteristics of the species of interest require clarification. In vitro conditions for the collection and selection of female and male gametes, oocyte maturation, sperm capacitation, co-incubation of gametes, and embryonic development can influence IVF results. Therefore, the present review considers the main advances in the methodologies already used for wild ungulates, emphasizing the strategies for improving the protocols to obtain better efficiency rates. Additionally, we discuss the conditions of each IVF stage, with emphasis on aspects related to in vitro manipulation and comparability with the protocols for domestic ungulates.

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Section: In Vitro Maturation Conditionsmentioning

confidence: 81%

Section: In Vitro Fertilization and Embryo Culturementioning

confidence: 99%

Section: In Vitro Maturation Conditionsmentioning

confidence: 99%

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Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Santos

Silva

Pereira

2022

Annals of Animal Science

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“…Further compounds also used include 6-dimethylaminopurine (6-DMAP) and puromycin ( Alberio et al , 2000 ; Heindryckx et al , 2008 ; Darwish and Magdi, 2015 ; Kim et al , 2015 ; Yeste et al , 2016a ; Aydinuraz et al , 2016 ; Capalbo et al , 2016 ; Nikiforaki et al , 2016 ; Yeste et al , 2016b ; Shang et al , 2019 ), while exposure to low concentrations of ethanol also elicits a single rise in Ca 2+ , which also seems to increase the rate of high-quality embryo and blastocyst formation from fresh and vitrified human oocytes, in vitro ( Zhang et al , 2017 ). The combination of multiple chemical mediators of AOA is able to produce embryos in animal models ( Borges et al , 2020a ). Ca 2+ ionophores, such as ionomycin or A23187, are the most commonly used form of AOA in IVF clinics today ( Ebner et al , 2012 ; D’Haeseleer et al , 2014 ; Ebner et al , 2015 ; Aydinuraz et al , 2016 ; Capalbo et al , 2016 ; Economou et al , 2017 ; Karabulut et al , 2018 ), and can work in conjunction with other compounds to enhance the processes important for fertility, such as acrosomal exocytosis ( Akter et al , 2019 ).…”

Section: Common Methods Of Aoamentioning

confidence: 99%

Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application

Kashir

Ganesh

Jones

et al. 2022

Human Reproduction Open

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Background Oocyte activation deficiency (OAD) is attributed to the majority of cases underlying failure of intracytoplasmic sperm injection (ICSI) cycles, the standard treatment for male factor infertility. Oocyte activation encompasses a series of concerted events, triggered by sperm-specific phospholipase C zeta (PLCζ), which elicits increases in free cytoplasmic calcium (Ca2+) in spatially and temporally specific oscillations. Defects in this specific pattern of Ca2+ release is directly attributable to most cases of OAD. Ca2+ release can be clinically mediated via assisted oocyte activation (AOA), a combination of mechanical, electrical and/or chemical stimuli which artificially promote an increase in the levels of intracytoplasmic Ca2+. However, concerns regarding safety and efficacy underlie potential risks that must be addressed before such methods can be safely widely used. Objective and Rationale Recent advances in current AOA techniques warrant a review of the safety and efficacy of these practices, to determine the extent to which AOA may be implemented in the clinic. Importantly, the primary challenges to obtaining data on the safety and efficacy of AOA must be determined. Such questions require urgent attention before widespread clinical utilisation of such protocols can be advocated. Search Methods A literature review was performed using databases including PubMed, Web of Science, Medline etc. using assisted oocyte activation, oocyte activation deficiency, calcium ionophores, intra-cytoplasmic sperm injection, phospholipase c zeta, oocyte activation, failed fertilisation, and fertilisation failure as keywords. Relevant articles published until June 2019 were analysed and included in the review, with an emphasis on studies assessing large scale efficacy and safety. Outcomes Contradictory studies on the safety and efficacy of AOA do not yet allow for the establishment of AOA as standard practice in the clinic. Heterogeneity in study methodology, inconsistent sample inclusion criteria, non-standardised outcome assessments, restricted sample size and animal model limitations render AOA strictly experimental. The main scientific concern impeding AOA utilisation in the clinic is the non-physiological method of Ca2+ release mediated by most AOA agents, coupled with a lack of holistic understanding regarding the physiological mechanism(s) underlying Ca2+ release at oocyte activation. Limitations, reasons for caution The number of studies with clinical relevance using AOA remains significantly low. A much wider range of studies examining outcomes using multiple AOA agents are required. Wider Implications In addition to addressing the five main challenges of studies assessing AOA safety and efficacy, more standardized, large-scale, multi-centre studies of AOA, as well as long term follow-up studies of children born from AOA, would provide evidence for establishing AOA as a treatment for infertility. The delivery of an activating agent that can more accurately recapitulate physiological fertilisation, such as recombinant PLCζ, is a promising prospect for the future of AOA. Further to PLCζ, many other avenues of physiological oocyte activation also require urgent investigation to assess other potential physiological avenues of AOA. Study Funding/Competing Interests DG was supported by Stanford University’s Bing Overseas Study Program. JK was supported by a Healthcare Research Fellowship Award (HF-14-16) made by Health and Care Research Wales (HCRW), alongside a National Science, Technology, and Innovation plan (NSTIP) project grant (15-MED4186-20) awarded by the King Abdulaziz City for Science and Technology (KACST). The authors have no competing interests to declare. Lay Summary At fertilisation, oocyte activation is triggered by sperm-specific phospholipase C zeta (PLCζ) by releasing calcium in specific patterns within the oocyte. A deficiency in this process underlies most cases of fertilisation failure in mammals. This process of calcium release can be clinically mimicked via assisted oocyte activation (AOA), involving a combination of mechanical, electrical and/or chemical stimuli. Recent advances in AOA techniques warrant a review of the safety and efficacy of these practices and of how AOA may be clinically implemented in the clinic. Herein, following a detailed literature review examining studies assessing large scale efficacy and safety, the main concern impeding clinical AOA implementation is its non-physiological nature, coupled with a lack of holistic understanding of physiological mechanism(s) underlying calcium release at fertilisation. We find that numerous questions require urgent attention before widespread clinical utilisation of such protocols can be advocated. We hope that this article will be able to aid the burgeoning number of researchers investigating the clinical efficacy of such methodology in further refining the practice until large scale utilisation can be achieved and accepted.

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“…In recent years, our group has developed steps to implement the SCNT technique in collared peccaries. This path has been developed since the establishment of the ideal environment for receptor oocytes (cytoplasts, Borges et al, 2018b), and the development of an efficient oocyte artificial activation protocol to provide the development of the reconstructed embryo (Borges et al, 2020b). In parallel, we established somatic tissue banks (Borges et al, 2017(Borges et al, , 2018aQueiroz Neta et al, 2018), somatic cell banks (Lira et al, 2020), and, recently, we established skin-derived fibroblasts lines (Borges et al, 2020a).…”

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confidence: 99%

Effects of Incubation Time and Method of Cell Cycle Synchronization on Collared Peccary Skin-Derived Fibroblast Cell Lines

Borges

Luciano

Nascimento

et al. 2021

Annals of Animal Science

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The success of cloning by somatic cell nuclear transfer depends on the efficiency of nuclear reprogramming, with the cycle stage of the donor cell playing a crucial role. Therefore, the aim was to evaluate three different approaches for cell cycle synchronization: (i) serum starvation (SS) for 1 to 4 days, (ii) contact inhibition (CI) for 1 to 3 days, and (iii) using cell cycle regulatory inhibitors (dimethyl sulfoxide, cycloheximide, cytochalasin B, or 6-dimethylaminopurine) for 1 and 2 days, in terms of their effects on synchronization in G0/G1 phases and viability of collared peccary skin fibroblasts. Flow cytometry analysis revealed that SS for 4 days (79.0% ± 1.6) and CI for 3 days (78.0% ± 1.4) increased the percentage of fibroblasts in G0/G1 compared to growing cells GC, (68.1% ± 8.6). However, SS for 3 and 4 days reduced the viability evaluated by differential staining (81.4% ± 0.03 and 81.6% ± 0.06) compared to growing cells (GC, 95.9% ± 0.06). CI did not affect the viability at any of the analyzed time intervals. No cell cycle inhibitors promoted synchronization in G0/G1. These results indicate that CI for 3 days was the most efficient method for cell cycle synchronization in peccary fibroblasts.

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Production of collared peccary (Pecari tajacu Linnaeus, 1758) parthenogenic embryos following different oocyte chemical activation and in vitro maturation conditions

Cited by 7 publications

References 45 publications

Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Embryo production by in vitro fertilization in wild ungulates: progress and perspectives – A Review

Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application

Effects of Incubation Time and Method of Cell Cycle Synchronization on Collared Peccary Skin-Derived Fibroblast Cell Lines

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