Production of clove extract loaded pullulan and whey protein nanofibers as antioxidant and antibacterial agent

Abstract

In this study, clove extract loaded nanofibers consisting of pullulan and whey protein were produced by electrospinning method in order to improve antibacterial and antioxidant activity. Diameters of the produced nanofibers varied between 215.7 and 536.8 nm. Fourier Transform Infrared Spectroscopy (FTIR), X‐ray diffraction (XRD) and differential scanning calorimeter (DSC) results showed that there was a close interaction between the obtained nanofibers and clove extract. Although the antioxidant activity of the nanofibers varied between 1535 and 4174 μmol TE/g dm, the antioxidant activity of the clove extract loaded nanofibers tended to increase when compared to their polymer solutions. The total phenolic content of the nanofiber samples varied between 4.93 and 45.05 mg GAE/g dm. It has been determined that clove extract loaded nanofibers showed antibacterial activity against Staphylococcus aureus (ATCC29113) and Micrococcus luteus (NCIMB). As a result, it was seen that the addition of clove extract improved the antioxidant and antibacterial properties of the fibers and these fibers could be a potential coating and packaging material that could have a potential use in food preservation.

Black Carrot Extract Containing Polyvinyl Alcohol-Based Nanofibers: Structural Characterization and Determination of Total Oxidant-Antioxidant Capacity

“…(2) and black carrot extract coated with PVA+metal (3) are as shown in table 2. In a study by Zambak et al (2022) nanofibers without clove extract did not show any antioxidant activity. Similarly, Aslaner et al ( 2021) stated that no antioxidant activity could be detected in extract free nanofibers.…”

“…When we look at the oxidant levels, black carrot extract is at a fairly low level, and the fact that the value is reset in nanocombined structures was evaluated as a very important data. Zambak et al (2022) stated that encapsulation of clove extract with pullulan which is a water-soluble polymer increased its water solubility and as a result increased antioxidant activity. Since PVA is also water soluble polymer, results could be also attributed to higher water solubility of nanostructures compared to pure extract.…”

ผลของสเปรย์จากสารสกัดสมุนไพรในการยับยั้งเชื้อแบคทีเรียในห้องส้วมสาธารณะ

“…จากการกดีชั กโค์รก (4) การใช้ เค์ร่ � องเป่ าม่ อ (Hand dryer) (18) (10,(21)(22) วิั สุดีุ ทางชี วิภาพเพ่ � อการรั กษ์าการติิ ดีเช่ � อ (23) ยาติ้ านเช่ � อจุ ลิ นทรี ย์ ใน ทางการแพทย์ (24)(25) และทั นติกรรม (26) และสุารยั บยั � งเช่ � อราใน ผลิ ติผลทางการเกษ์ติรหลั งการเก็ บเกี � ยวิ (13,19)…”