Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion

Mahata, Maria Endo; Shinya, Shoko; Masaki, Eiko; Yamamoto, Takashi; Ohnuma, Takayuki; Brzeziński, Ryszard; Mazumder, Tapan Kumar; Yamashita, K.; Narihiro, Kazue; Fukamizo, Tamo

doi:10.1016/j.carres.2013.06.002

Cited by 26 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mass spectrometry (MS) techniques, such as LC-MS/MS (Kim et al 2013) or MALDI-TOF (Montilla et al 2013), are of great potential to identify (or to discard) the formation of certain COS in the reactions, especially combined with NMR spectroscopy (Mahata et al 2014). Figure 3 illustrates the ESI-Q-TOF MS spectrum that resulted from the hydrolysis of chitosan CHIT600 with Neutrase 0.8L.…”

Section: Identification Of Cos By Mass Spectrometrymentioning

confidence: 99%

Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties

Santos-Moriano

Fernández‐Arrojo

Mengíbar³

et al. 2017

Biocatalysis and Biotransformation

View full text Add to dashboard Cite

Among several commercial enzymes screened for chitosanolytic activity, Neutrase 0.8L (a protease from Bacillus amyloliquefaciens) was selected in order to obtain a product enriched in deacetylated chitooligosaccharides (COS). The hydrolysis of different chitosans with this enzyme was followed by size exclusion chromatography (SEC-ELSD), mass spectrometry (ESI-Q-TOF), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Neutrase 0.8L converted 10 g/L of various chitosans into mostly deacetylated oligosaccharides, yielding approximately 2.5 g/L of chitobiose, 4.5 g/L of chitotriose and 3 g/L of chitotetraose. We found out that the neutral protease was not responsible of the chitosanolytic activity in the extract, whilst it could participate in the deacetylating process. The synthesized COS were tested in vitro for their neuroprotective (towards human SH-S5Y5 neurons) and anti-inflammatory (in RAW macrophages) activities, and compared with other functional ingredients, namely fructooligosaccharides.

show abstract

Section: Identification Of Cos By Mass Spectrometrymentioning

confidence: 99%

Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties

Santos-Moriano

Fernández‐Arrojo

Mengíbar³

et al. 2017

Biocatalysis and Biotransformation

View full text Add to dashboard Cite

show abstract

“…N174 [82]. Valuable hetero- and homo-oligosaccharides GlcN–GlcNAc, (GlcN) 2 –GlcNAc, and (GlcN) 2 were produced, functionally, by the enzymatic digestion of the intact cells.…”

Section: Bioproduction Of Chitosan Oligosaccharides and Its Monomermentioning

confidence: 99%

Bioproduction of Chitooligosaccharides: Present and Perspectives

2014

View full text Add to dashboard Cite

Chitin and chitosan oligosaccharides (COS) have been traditionally obtained by chemical digestion with strong acids. In light of the difficulties associated with these traditional production processes, environmentally compatible and reproducible production alternatives are desirable. Unlike chemical digestion, biodegradation of chitin and chitosan by enzymes or microorganisms does not require the use of toxic chemicals or excessive amounts of wastewater. Enzyme preparations with chitinase, chitosanase, and lysozymeare primarily used to hydrolyze chitin and chitosan. Commercial preparations of cellulase, protease, lipase, and pepsin provide another opportunity for oligosaccharide production. In addition to their hydrolytic activities, the transglycosylation activity of chitinolytic enzymes might be exploited for the synthesis of desired chitin oligomers and their derivatives. Chitin deacetylase is also potentially useful for the preparation of oligosaccharides. Recently, direct production of oligosaccharides from chitin and crab shells by a combination of mechanochemical grinding and enzymatic hydrolysis has been reported. Together with these, other emerging technologies such as direct degradation of chitin from crustacean shells and microbial cell walls, enzymatic synthesis of COS from small building blocks, and protein engineering technology for chitin-related enzymes have been discussed as the most significant challenge for industrial application.

show abstract

“…Rhizopus sp. can synthesize oligosaccharides, which have a variety of physiological functions and can be used for the production of health foods . There are also other species that could produce a series of enzymes such as lipases, proteases and amylases (Fig.…”

Section: The Application Of Microorganisms In Liquor Brewingmentioning

confidence: 99%

The brewing process and microbial diversity of strong flavour Chinese spirits: a review

et al. 2017

View full text Add to dashboard Cite

Chinese Baijiu (Chinese spirit or Chinese liquor) is one of the six major distilled spirits in the world, and is renowned overseas. Chinese Baijiu has a long history of more than 1000 years when people first begin to brew liquor using distillation. So far, Chinese Baijius have formed 12 types according to their flavour characteristics. The strong flavour Chinese spirit is regarded as one of the typical representatives, and occupies a > 70% market share in China. The liquor brewing process is under an open environment, with grains as the fermentation substrates, as well as Daqu as the saccharifying ferment. The brewing process is complex and impacted by multiple factors. Among the factors, the microorganisms have important influences on the brewing process and product quality of liquors. In the past decades, a series of research achievements have been made with strong flavour Chinese spirits. However, reviews related to this field are relatively few. In this paper, we have reviewed strong flavour Chinese spirits including the introduction, brewing process, microorganism diversity of Daqus, fermented grains and pit muds, and the application of microbes.

show abstract

Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion

Cited by 26 publications

References 25 publications

Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties

Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties

Bioproduction of Chitooligosaccharides: Present and Perspectives

The brewing process and microbial diversity of strong flavour Chinese spirits: a review

Contact Info

Product

Resources

About