Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation

Leite, Rodrigo Simões Ribeiro; Bocchini, Daniela Alonso; Martins, Eduardo da Silva; Silva, Dêenis; Gomes, Eleni; Silva, Roberto da

doi:10.1007/s12010-007-9058-y

Cited by 20 publications

(9 citation statements)

References 32 publications

(38 reference statements)

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“…This inhibition halo evidenced the diffusion of metabolites. We hypothesized that the secretion of metabolites and enzymes like cellulolytic and hemicellulolytic enzymes (Leite et al 2007) and the capacity of this fungus to form biofilms (Metzger et al 2006) could have contributed to the success of the endophyte controlling R. solani. In future, the microscopic characterization of the hyphal interactions should be performed to further characterize the fungal interactions.…”

Section: Discussionmentioning

confidence: 99%

Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem

et al. 2012

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We studied the diversity and biocontrol potential of 100 fungal endophytes isolated from Espeletia spp., endemic plant species from the Paramo in the Andean mountain range. Our sample was genotypically highly diverse at all ITS similarity levels. The antagonistic properties of these isolates were tested against common crop pathogens in Colombia, including Pectobacterium carotovorum, Ralstonia solanacearum, Pseudomonas syringae, Xanthomonas campestris, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, and Phytophthora infestans. All endophytic isolates were able to significantly inhibit the growth of at least one of the plant pathogens tested (P \ 0.05). Three main types of endophyte/pathogen interactions were observed.However, only those endophytes that produced an evident inhibition halo were further studied using their crude extracts to confirm that the inhibitory effect was due to the production of endophytic bioactive metabolites. From these experiments, nine promising isolates were selected for co-inoculation tests with R. solani in tomato plants. The isolates identified as Aureobasidium pullulans and Paraconiothyrium sporulosum not only protected the plants against this pathogen but also allowed them to exhibit similar growth and development as the uninoculated control. This work explores new alternatives for disease management without the application of chemical pesticides.

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Section: Discussionmentioning

confidence: 99%

Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem

et al. 2012

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“…They were then paper filtered (Whatman, no. 1) and centrifuged at 1232.6 × g for 5 min (ITR, Brazil), yielding the crude enzyme extract from the supernatant to be used for the determination of enzyme activity [21].…”

Section: Aqueous Enzymatic Extractionmentioning

confidence: 99%

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Silva

et al. 2020

SN Appl. Sci.

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Lichtheimia ramosa is a promising candidate fungus for solid state bioprocesses (SSB) due its rapid colonization. Fruit wastes present ideal conditions for fungal growth and biotransformation, which can promote the release of products of biotechnological interest. Thus, the aim of this work was to evaluate the mycelial growth and the enzymatic activities of L. ramosa in pineapple (Ananas comosus), orange (Citrus sinensis), mango (Mangifera indica), passion fruit (Passiflora edulis) and grape (Vitis vinifera) fruit wastes via SSB, and their influence in the generation of molecules with potential use. The SSB was evaluated in terms of capacity of biotransformation of the substrate (composition, protein enrichment, and fatty acid profile) and production of amylase, carboxymethylcellulase (CMCase), xylanase and lipase enzymes. Main cultivations were carried out at 30 °C for 30 days and every 5 days samples were taken and analyzed for microbiological content, proximal composition and enzymatic profile. Fatty acids were determined at day 0 (baseline) and at the end of the cultivations. L. ramosa grew well in all substrates up to the 25th day, except for on the orange residue, upon which development was slightly lower at this time. Protein enrichment was found in all substrates as follows: passion fruit (309.54%), pineapple (294.89%), mango (263.45%), orange (65.60%) and grapes (19.17%). Regarding enzymes, lipase was not synthetized in any of the substrates, though amylase, CMCase and xylanase were observed at different levels. The fatty acid profile varied from raw to cultivated substrates, indicating that L. ramosa can act in the synthesis and conversion of these acids. It was concluded that L. ramosa and the studied substrates are viable for SSB.

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“…The microorganisms were placed on an orbital shaker (Marconi, Brazil) (130 rpm, 35ºC, 30 min), paper filtered (Whatman, no. 1), and then centrifuged (ITR, Brazil) (3000 rpm, 5 min), yielding the crude enzyme extract for the determination of enzyme activity (adapted from Leite et al 2007). The supernatant crude enzyme extract was used for determination of enzyme activities.…”

Section: Aqueous Enzymatic Extractionmentioning

confidence: 99%

“…The enzymatic reaction was stopped with 2 mL of sodium carbonate 2 M (Impex), and the resulting product was quantified spectrophotometrically (Bioespectro, Brazil) at 410 nm (Leite et al 2007). One unit of enzyme activity was defined as the amount of enzyme able to release 1 μmol of nitrophenol per minute of reaction.…”

Section: Amylase Cmcase and Xylanase Activitiesmentioning

confidence: 99%

Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocesses

Silva¹,

Lacerda²,

Leite³

et al. 2013

Electron. J. Biotechnol.

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Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative for costs reduction and to avoid their disposal into environment. The aim of this work was to evaluate the physiology of the fungus Lichtheimia ramosa in terms of microbial growth and production of amylases, β-glucosidases, carboxymethylcellulase (CMCase), and xylanases, via SSB, utilizing wastes of the Brazilian savannah fruits bocaiuva (Acrocomia aculeata), guavira (Campomanesia pubescens) and pequi (Caryocar brasiliense) as substrate at different temperatures (25, 30, and 35ºC) during 168 hrs. Results: Samples were taken every 24 hrs, which resulted in 8-points kinetic experiments to determine microbiological and enzymatic contents. The best substrate for β-glucosidase activity was pequi waste after 48 hrs at 30ºC (0.061 U/mL). For amylase activity, bocaiuva presented itself as the best substrate after 96 hrs at 30ºC (0.925 U/mL). CMCase activity was higher in guavira waste after 96 hrs at 35ºC (0.787 U/mL). However, the activity was more expressive for xylanase in substrate composed of bocaiuva residue after 144 hrs at 35ºC (1.802 U/mL). Conclusions: It was concluded that best growth condition for L. ramosa is at 35ºC for all substrates and that xylanase is the enzyme with more potential in SSB, considering the studied Brazilian savannah fruit wastes.

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Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation

Cited by 20 publications

References 32 publications

Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem

Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem

Biotransformation of fruit residues via solid state bioprocess using Lichtheimia ramosa

Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocesses

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