Production of camptothecin in the elicited callus cultures of Nothapodytes nimmoniana (J. Graham) Mabberly

Influence of plant growth regulators on morphogenic response, biomass and camptothecin production in the callus cultures of Chonemorpha fragrans (Moon) Alston

“…However, yield of CPT in the callus cultures were reduced to about half the productivity obtained on solid medium cultivation system but, overall productivity relatively higher (Fig. 2B-F), despite the possibility of degradation of the leached CPT in liquid medium cultures [19,71]. For evaluation of the influence of PGRs on biomass production and biosynthesis of CPT in the C. fragrans callus cultures, absolute biomass production in callus obtained from in vitro morphogenesis study and CPT production were evaluated by the measured fresh and dried weight (after dried to constant mass), the alkaloid extracted and HPTLC analysis performed.…”

“…However, allosecurinine level was negatively affected while PGR-free medium cultivation resulted in low production of the indolizidine alkaloids, suggesting the substantial effect of PGRs on biosynthesis of alkaloids [43]. In previous study with the N. nimmoniana callus cultures, biomass and CPT production were influenced by the culture medium type and cultivation duration [19]. In the study, significant proportion of the synthesized CPT their combination with auxins or auxins-alone, and concentrations supplemented in the culture medium influenced the levels of securinega-type alkaloids and biomass production in a differential manner [74].…”

“…In the present experiment, for each subculture cycle of the seed embryo-derived callus cultures, which is during the in vitro morphogenesis study (using solid or in liquid medium cultivation systems), callus samples were harvested, its weight taken in fresh form and after dried to constant mass in an oven at 70 o C. In the case of liquid medium cultivation, callus masses were inoculated into volume of autoclaved liquid medium contained in 50 mL capacity conical flasks, and the cultures kept on orbital shaker operated at 60 rpm for 12 h/day. Fresh and absolute biomass were calculated from the relationship of callus fresh to dry weight given by the formulas: [19,20,21]. CPT yield of the tissues and culture medium were evaluated as reported earlier [22] through the use of standard curve that had linear regression line equation Y = 0.0018 X +7.2184, and expressed in percentages for the tissues used without organogenesis study and in mg/g calli DW or mg/mL medium for others obtained from the organogenesis study (experiments were carried out at different times).…”

Production of Camptothecin from Nothapodytes nimmoniana: An Overview

“…One of the approaches is to develop a strategy for large scale propagation of the plant without affecting natural resources [3,25] . Many researchers have investigated different geographical locations across India to find out the better species for high CPT yield through clonal propagation [26][27][28] . Many in vitro techniques are engaged for the enhanced yield of CPT such as media optimization, selection of high yielding cell lines, elicitation using different biotic and abiotic elicitors and hairy root cultures.…”

“…Low doses of gamma radiation found to increase product quantity by 20 folds than non-irradiated callus cultures at 20Gy. Isah [27] was reported the elicitation of CPT production in hypocotyl derived calli with yeast extract (YE) and vanadyl sulfate (VS), both the elicitors enhanced CPT production more in liquid medium than in solid medium; YE increased the production by 4.2 fold whereas VS increased the production by 3.03 fold in liquid medium.…”