Production of butanol from glucose and xylose with immobilized cells of Clostridium acetobutylicum

Chen, Yong; Zhou, Tao; Liu, Dong; An, Li; Xu, Shijie; Liu, Qingguo; Li, Bingbing

doi:10.1007/s12257-012-0573-5

Cited by 69 publications

(34 citation statements)

References 22 publications

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“…Similarly, for C. acetobutylicum butanol yields when cells were immobilized on pretreated cotton towels were 28.3% higher than those from planktonic cells [103]. The kosmotropic activities of immobilization polymers also appears to confer tolerance to other stress parameters that disorder macromolecular systems, including high temperature [102 ] Immobilization matrices can restrict the mass transfer of nutrients, products, gases and other metabolites to and from the cells resulting in reduced growth rates [93].…”

Section: Type Of Interventionmentioning

confidence: 99%

“…Indeed, polymers currently used for immobilization are known to induce compatible-solute accumulation (Table 2) [11 ,102 ]. Immobilization of solventogenic Clostridium species onto different ligands such as brick, zeolite, and alginates has been demonstrated to be viable as a technique for improving butanol tolerance and production (Table 2) [103,104] due, in part, to formation of biofilms which promote adhesion, water retention, and production of protective barriers. Clay particles can retain water [10 ], and the extracellular polymeric substances of biofilms are kosmotropic [7 ], so these materials may also induce stress adaptations.…”

Section: Removal Of Inhibitory Products and Use Of Cell-free Systemsmentioning

confidence: 99%

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Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Cray

Stevenson

Ball

et al. 2015

Current Opinion in Biotechnology

165

175

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Section: Type Of Interventionmentioning

confidence: 99%

Section: Removal Of Inhibitory Products and Use Of Cell-free Systemsmentioning

confidence: 99%

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Cray

Stevenson

Ball

et al. 2015

Current Opinion in Biotechnology

165

175

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“…The immobilized cells can maintain high viable cell densities in a reactor and eliminates the cellular lag phase to increase the productivity due to the possibility of sustaining higher dilution rates [18]. Moreover, the immobilized cell reactors might enhance the tolerance to adverse conditions such as butanol toxicity [19,20].…”

Section: Introductionmentioning

confidence: 99%

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

Bankar

Jurgens

Survase

et al. 2014

Fuel

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Highlights Metabolic engineering to convert acetone into isopropanol  Continuous immobilized fermentation to validate industrial feasibility of strain  Optimization of bioprocess to enhance the solvent yield and productivity  Utilization of lignocellulosic spent liquor as a substrate for the fermentation AbstractThe co-production of acetone during traditional butanol fermentation (acetone-butanol-ethanol) lowers the yield of alcohol biofuels. The present study was aimed to develop an improved Clostridium acetobutylicum strain with enhanced alcohol fuel production capability. The previously developed IBE producing C. acetobutylicum DSM792-ADH strain was further optimized to enhance the solvent productivity and yield. Immobilized cell column reactor was used to investigate the effect of dilution rate on IBE fermentation. Pure glucose, artificial sugar mixture and SEW spent liquor from spruce chips were used as substrates to study the behavior of the modified microorganism. The highest total solvent concentrations of approximately10.60 g.l -1 , 10 g.l -1 and 6 g.l -1 were obtained when glucose, sugar mixture and SEW spent liquor were used as substrate respectively. The modified microorganism could effectively utilize the lignocellulosic biomass hydrolyzate (SEW spent liquor) to yield a solvent productivity of 1.67 g.l -1 .h -1 .

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“…However, the effects of excessive initial sugar concentration were mitigated in the IC-TSC system, which proved more efficient than the SC system. Immobilized cells were also more tolerant to butanol toxicity than their free counterparts [25]. In previous reports of ABE fermentation, solventogenesis was shown to be favored at high cell densities, whereas acidogenesis was favored at low cell densities [26].…”

Section: Effects Of Initial Sugar Concentrations On Abe Production Bymentioning

confidence: 83%

Enhanced acetone-butanol production from sugarcane juice by immobilized Clostridium acetobutylicum (ATCC 824) on thin-shell silk cocoons

Kittithanesuan

Phisalaphong

2015

Biotechnol Bioproc E

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To promote its performance during acetonebutanol-ethanol (ABE) fermentation, Clostridium acetobutylicum (ATCC 824) was immobilized on a thin-shell silk cocoon (TSC). As a residual from the silk industry, TSC offers a cheap, biocompatible support material. The adsorbed C. acetobutylicum cells digested the TSCs into amino acids as a nitrogen source. It was shown that TSC might promote the phase shift to acetone in the ABE fermentation. At an initial reducing sugar concentration of 90 g/L, the ABE productivity of the immobilized cell culture on TSC (IC-TSC) in batch fermentation was 0.18 g/L/h, and the solvent mixture comprised 6.1 g/L acetone, 15.9 g/L butanol, and 1.9 g/L ethanol. Repeated 4-cycle batch fermentation using IC-TSC significantly improved the ABE productivity. After 48 h of cyclic fermentation, the maximum ABE productivity was 0.43 g/L/h with acetone, butanol and ethanol concentrations of 6.6, 12.9, and 1.1 g/L, respectively.

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Production of butanol from glucose and xylose with immobilized cells of Clostridium acetobutylicum

Cited by 69 publications

References 22 publications

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

Enhanced acetone-butanol production from sugarcane juice by immobilized Clostridium acetobutylicum (ATCC 824) on thin-shell silk cocoons

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