Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Cray, Jonathan A.; Stevenson, Andrew; Ball, Philip; Bankar, Sandip B.; Eleuthério, Elis C. A.; Ezeji, Thaddeus Chukwuemeka; Singhal, Rekha S.; Thevelein, Johan M.; Timson, David J.; Hallsworth, John E.

doi:10.1016/j.copbio.2015.02.010

Cited by 168 publications

(183 citation statements)

References 218 publications

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“…Apart from the entrapment of the bacterial cells inside the beads, the immobilization into porous matrix such as polysaccharide gel can result in the bacterial cells moving to the outer surface of the beads. However, the effect the immobilization may influence the water activity, and as consequence metabolism will be affected [24]. In Figure 3, a 3D response surface plot was constructed to illustrate the synergistic effects of Naalginate and CaCl2 concentrations on the response (CaCO3) and also provide a visual interpretation for the location of the optimal concentrations.…”

Section: Bacterially Induced Caco3 Precipitationmentioning

confidence: 99%

The Effect of Cell Immobilization by Calcium Alginate on Bacterially Induced Calcium Carbonate Precipitation

et al. 2017

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Abstract:Microbially induced mineral precipitation is recognized as a widespread phenomenon in nature. A diverse range of minerals including carbonate, sulphides, silicates, and phosphates can be produced through biomineralization. Calcium carbonate (CaCO3) is one of the most common substances used in various industries and is mostly extracted by mining. In recent years, production of CaCO3 by bacteria has drawn much attention because it is an environmentally-and healthfriendly pathway. Although CaCO3 can be produced by some genera of bacteria through autotrophic and heterotrophic pathways, the possibility of producing CaCO3 in different environmental conditions has remained a challenge to determine. In this study, calcium alginate was proposed as a protective carrier to increase the bacterial tolerance to extreme environmental conditions. The model showed that the highest concentration of CaCO3 is achieved when the bacterial cells are immobilized in the calcium alginate beads fabricated using 1.38% w/v Na-alginate and 0.13 M CaCl2.

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Section: Bacterially Induced Caco3 Precipitationmentioning

confidence: 99%

The Effect of Cell Immobilization by Calcium Alginate on Bacterially Induced Calcium Carbonate Precipitation

et al. 2017

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“…This study thereby revealed that some stressors, including MgCl 2 , can inhibit metabolism and cell multiplication via their chaotropic activity and by simultaneously imposing osmotic stress; a finding that is unprecedented for any type of living system. While not an osmotic stressor, it seems likely that ethanol can (at high concentrations) impose simultaneous chaotropicity and low water activityinduced stresses in S. cerevisiae (Hallsworth 1998;Cray et al 2015). Conversely, high concentrations of MgCl 2 may ultimately inhibit the multiplication of extremely halophilic bacteria and Archaea due to chaotropicity rather than water activity or osmotic stress (Hallsworth et al 2007;Yakimov et al 2015).…”

Section: Topics Covered In This Special Issuementioning

confidence: 93%

Fungal stress biology: a preface to the Fungal Stress Responses special edition

et al. 2015

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There is currently an urgent need to increase global food security, reverse the trends of increasing cancer rates, protect environmental health, and mitigate climate change. Toward these ends, it is imperative to improve soil health and crop productivity, reduce food spoilage, reduce pesticide usage by increasing the use of biological control, optimize bioremediation of polluted sites, and generate energy from sustainable sources such as biofuels. This review focuses on fungi that can help provide solutions to such problems. We discuss key aspects of fungal stress biology in the context of the papers published in this Special Issue of Current Genetics. This area of biology has relevance to pure and applied research on fungal (and indeed other) systems, including biological control of insect pests, roles of saprotrophic fungi in agriculture and forestry, mycotoxin contamination of the food-supply chain, optimization of microbial fermentations including those used for bioethanol production, plant pathology, the limits of life on Earth, and astrobiology.

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“…Cray et al [11] have written an excellent review, ''Chaotropicity: a key factor in product tolerance of biofuelproducing microorganisms'', on a subject of broad and fundamental importance for understanding and engineering tolerance to toxic chemicals. Metabolites that are desirable as fuel molecules, commodity or specialty chemicals are typically mildly to severely toxic to cells, thus inhibiting cell growth and/or viability and preventing the achievement of the necessary high product titers.…”

Section: Product Toxicity and Downstream Processingmentioning

confidence: 99%