Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone–butanol–ethanol fermentation using an empirical model

Shukor, Hafiza; Al-Shorgani, Najeeb Kaid Nasser; Abdeshahian, Peyman; Hamid, Aidil Abdul; Anuar, Nurina; Rahman, Norliza Abd

doi:10.1016/j.biortech.2014.07.055

Cited by 60 publications

(20 citation statements)

References 30 publications

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“…This is due to the fact that Clostridial cells decreases the lag phase of growth which improves the microbial cell growth to enhance the solventogenesis phase where the butanol produced. 6 A similar pattern was reported by AlShorgani et al (2015) who studied the production of butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using palm oil mill effluent. They observed that the increased in inoculum size from 5% However, the optimum inoculum size is varied depending on the microorganism and substrate used.…”

Section: Main Effects Analysissupporting

confidence: 56%

“…If there is a significant lack of fit, the response is not fitted. 6 As shown in Table 3, the F value for lack of fit with a value of 0.21 and a probability value of 0.8871 implied that lack of fit was insignificant and hence the model was valid for further studies.…”

Section: Analysis Of Variance (Anova)mentioning

confidence: 99%

“…In this study, the fermentation was performed at different inoculum sizes at 1% (v/v) and 20% (v/v) with the center point of 10.5% (v/v) of inoculum. Shukor et al (2014) reported that when there is an increasing in inoculum size, the biobutanol production in ABE fermentation was also increased. This is due to the fact that Clostridial cells decreases the lag phase of growth which improves the microbial cell growth to enhance the solventogenesis phase where the butanol produced.…”

Section: Main Effects Analysismentioning

confidence: 99%

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Factorial experimental design for biobutanol production from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824

Nasrah

Zahari

Masngut

et al. 2017

Chem. Eng. Res. Bull.

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Abstract:Biobutanol is an alternative energy that can be promising as the future energy source. It can be produced from natural and renewable agriculture wastes such as oil palm frond (OPF) juice by microbes. Clostridium acetobutylicum has the ability to ferment the sugars in OPF juice as carbon source into biobutanol. This research aimed to investigate the effect of independent and interaction factors; initial pH medium (5-7), inoculum size (1-20%), initial total sugars concentration (40-60 g/L), temperature (32-42°C) and yeast extract concentration (1-10 g/L) on the production of biobutanol from oil palm frond (OPF) juice by C. acetobutylicum ATCC 824 using a two level half factorial design which have been developed by the Design Expert Software Version 7.1. Based on the factorial analysis, it was observed that the most significant parameter was yeast extract concentration, which contributes 8.20%, followed by inoculum size and temperature, which were contribute 7.84% and 7.56%, respectively. The analysis showed the R 2 value for the model was 0.9805 and the interaction between inoculum size and temperature gave the highest influenced to the fermentation process with contribution up to 16.31%. From the validation experiments, the experimental values were reasonable close to the predicted values with only 5.87% and 10.09% of errors. It confirmed the validity and adequacy of the predicted models. Hence, the data analysis developed from the Design Expert Software could reliably predict biobutanol yields. This study indicated that each of the factors may affect the fermentation process of the biobutanol production.

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Section: Main Effects Analysissupporting

confidence: 56%

Section: Analysis Of Variance (Anova)mentioning

confidence: 99%

Section: Main Effects Analysismentioning

confidence: 99%

See 1 more Smart Citation

Factorial experimental design for biobutanol production from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824

Nasrah

Zahari

Masngut

et al. 2017

Chem. Eng. Res. Bull.

View full text Add to dashboard Cite

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“…The improving butanol production with the increasing inoculum size due to the fact that is because of an increase in the inoculation of Clostridial cells decreases the lag phase of microbial cell growth. This condition improves Clostridial growth resulting in the enhancement of solventogenesis phase and also the butanol production [23]. As can be seen in Fig.…”

Section: Optimization Of Biobutanol Productionmentioning

confidence: 77%

Statistical Optimization for Biobutanol Production byClostridium acetobutylicumATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology

et al. 2017

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Abstract. The interaction between incubation temperature, yeast extract concentration and inoculum size was investigated to optimize critical environmental parameters for production of biobutanol from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824 using response surface methodology (RSM). A central composite design (CCD) was applied as the experimental design and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for the biobutanol production. The incubation temperature, yeast extract concentration and inoculum size showed significant value at p < 0.005. The results of optimization process showed that a maximum biobutanol production was obtained under the condition of temperature 37 °C, yeast extract concentration 5.5 g/L and inoculum size 10%. Under these optimized conditions, the highest biobutanol yield was 0.3054 g/g after 144 hours of incubation period. The model was validated by applying the optimized conditions and 0.2992 g/g biobutanol yield was obtained. These experimental findings were in close agreement with the model prediction, with a difference of only 9.76%.

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“…By use of lignocelluloses as substrate, three components, including acetone, butanol and ethanol (ABE) are simultaneously produced, in which butanol is the major product (Ezeji et al, 2012;Jurgens et al, 2012;Wen et al, 2014a,b). Different biomass such as wheat straw (Quershi et al, 2007;Nanda et al, 2014), rice straw (Gottumukkala et al, 2013(Gottumukkala et al, , 2014, barley straw (Quershi et al, 2010a), corn stover (Parekh et al, 1988;Quershi et al, 2010b), corn cob and fibers (Marshal et al, 1992;Guo et al, 2013), palm kernel cake (Shukor et al, 2014), cassava starch (Li et al, 2014a,b), pinewood and timothy grass (Nanda et al, 2014), switch grass (Quershi et al, 2010b;Gao et al, 2014), sag pith (Linggang et al, 2013) and dried distillers' grains have been used as substrates for ABE fermentation by numerous Clostridium strains such as C. acetobutylicum, C. aurantibutyricum, C. beijerinckii , C. cadaveris, C. pasteurianum, C. saccharoperbutylacetonicum, C. saccharobutylicum, C. sporogenes and C. tetanomorphum (Inui et al, 2008;Quershi et al, 2013). This process commonly occurs in two phases, including acidogenic phase where acetic and butyric acids are produced, and solventogenic phase where acids are used and ABE are generated.…”

Section: Cbp In Biobutanol Productionmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone–butanol–ethanol fermentation using an empirical model

Cited by 60 publications

References 30 publications

Factorial experimental design for biobutanol production from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824

Factorial experimental design for biobutanol production from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824

Statistical Optimization for Biobutanol Production byClostridium acetobutylicumATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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