Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to study the effect of different pH, temperature, enzyme loading and reaction time in order to identify the best working conditions to produce the highest yield of sugar. Prior to enzymatic hydrolysis, the pineapple leaves were subjected to hydrothermal pretreatment in order to obtain the hydrolysate liquid. Enzymatic hydrolysis was then carried out by using endo-1,4-xylanase and the reducing sugar was analyzed by using dinitrosalicylic (DNS) method. The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively.
Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem. Due to high fiber content, pineapple wastes are unsuitable for animal feed. However, the fermented waste juice could be used to produce enzymes. The objective of this study is to produce xylanase and cellulase from the fermentation of pineapple waste using Lactobacillus casei (L. casei) bacteria inoculated from probiotic drink. The fermentation was performed using different screening parameters (incubation time, temperature, pH value and substrate concentration) according to Two-Level Full Factorial Design (FFD) by Design Expert. From this study, the incubation temperature and substrate concentration had the highest influence on the xylanase activity (39.82 U/mL) while the fermentation time and substrate concentration mostly affected the cellulase activity (8.05 U/mL). Meanwhile, the pH had the least influence on both enzyme activities. The pineapple waste at its best fermentation parameters not only offers an economical way of high enzyme production but also alleviates the agricultural waste disposal issue. Further optimization of the pineapple waste fermentation parameters is required though to maximize enzyme production.
Pineapple fruit contains essential minerals and vitamins with some medicinal values. Hence, it can be used as a raw material to make poultry supplements. Abundance of pineapple waste also has led to the environmental problems such as producing bad odor and has increased waste capacity in the landfill. In this present work, the potential of pineapple fruit and pineapple waste were investigated to be used as the raw materials for poultry supplement based on their enzyme activities. In this study, the effect of fermentation time and type of pineapple substrates on enzyme activities which were xylanase, total cellulase and protease were studied. Two types of pineapple samples have been used in this study which were pineapple fruit and pineapple waste. Four type of pineapple substrates have been prepared which were pineapple juice, pineapple waste, pineapple juice + probiotic and pineapple waste + probiotic. The purpose of adding probiotic in this study was to enhance the fermentation reaction. The results showed that the fermentation time that have the highest enzyme activities for xylanase, total cellulase and protease were observed at day 2, 4 and 6 respectively, where substrate contains pineapple juice + probiotic recorded the highest enzyme activities value for all the three enzymes. This study had investigated that pineapple fruit and pineapple waste contain digestion enzymes which were xylanase, protease and cellulase that can be applied as poultry supplements.
One of the feasible approaches to oversee pineapple waste deposit without harming the environment is by converting these build-ups into value added items such as biovinegar. The objective of this work is to screen the fermentation parameter to identify the best condition and significant parameters affecting the fermentation. Five independent parameters were investigated, namely; temperature, fermentation time, addition of glucose, part and condition of waste. Fractional factorial design of Design Expert® software was used to investigate the effect of independent parameters as well as the interaction between parameters on the biovinegar production. The work was carried out by natural fermentation in which naturally occurred microorganism readily available on the raw materials (pineapple waste) was used. The result showed that the order of parameter significance in acid production was as follows: temperature > addition of glucose > fermentation time > part of waste > condition of waste. The interaction parameter of fermentation time and addition of glucose had the strongest effect on the acid production. The best fermentation condition was carried out using pineapple peel juice at 30 °C for 8 days in an anaerobic condition with 50 g/L glucose addition. Under these conditions, acid production was 1.12 % w/v in which acetic acid concentration was 0.94 % w/v. The product pH was recorded at 3.57. The product yield and productivity were recorded at 0.1699 g/g and 0.0489 g/L.h, respectively. Exploration on producing biovinegar using mixed strains and pineapple waste as substrate could be another way to reduce environmental pollution and at the same time turning this waste into value added product. Moreover, using the natural fermentation together with the carry over benefit of the pineapple benefitted the quality of produced biovinegar.
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