Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

Abstract

Summary This study demonstrated a biotechnological approach for simultaneous production of low‐cost H2, liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium (Clostridium beijerinckii) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H2 h−1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L−1, respectively. ABE productivity (g L−1 h−1) was 0.0486 and 0.0564 with a yield rate (g g−1) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials.

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

“…In recent decades, due to the alarming increase in motorization, industrialization and human population; mankind faces the inevitable depletion in global strategic petroleum reserves on earth 1 , 2 . As well as the rapid increase in petroleum prices and alarming environmental crises makes bioenergy production from renewable raw materials is considered a sustainable alternative energy source for the future 3 , 4 .…”

What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

“…Besides homopolymers, some microorganisms were found to be able to synthesize scl-mcl-PHA copolymers using molasses as the only carbon source. Hassan et al [ 70 ] observed that Clostridium beijerinckii ASU10 (KF372577) has the potential to produce P(3HB-co-3HO) in the amount of 37.93% of CDW. Gas chromatography–mass spectrometry (GC-MS) analysis confirmed that the extracted copolymer consisted of 47.3 mol% of 3HB and 52.7% of 3HO.…”

Improved dark fermentation of cane molasses in mesophilic and thermophilic anaerobic fluidized bed reactors by selecting operational conditions

“…2 In addition to large quantities of total sugars, molasses is also a source of nitrogen and vitamins. 3 Thus, molasses is a cheap carbohydrate that can be used as a resource on the fermentative generation of butyrate, 3 butanol, 4 hydrogen, [5][6][7][8][9] and succinate. 10 Succinic acid is generated as an intermediate metabolite in the tricarboxylic metabolite cycle.…”