What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

Marciniak, Paulina; Możejko-Ciesielska, Justyna

doi:10.3390/polym13111731

Cited by 15 publications

(11 citation statements)

References 113 publications

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“…Biodegradation is a natural and complex process of decomposition facilitated by biochemical mechanisms and the successive mineralization of the polymer material. Biodegradable plastics open the way for new waste management strategies since these materials are designed to degrade under environmental conditions or in municipal and industrial biological waste treatment facilities [ 63 ]. Bacteria, fungi, and actinomycetes are of particular interest in the biodegradation of natural and synthetic polymers.…”

Section: Biodegradationmentioning

confidence: 99%

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

et al. 2022

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Biodegradation is necessary for water-soluble or water-immiscible polymers because they eventually enter streams which can neither be recycled nor incinerated. It is important to consider the microbial degradation of natural and synthetic polymers in order to understand what is necessary for biodegradation and the mechanisms involved. Low/high-density polyethylene is a vital cause of environmental pollution. It occurs by choking the sewer line through mishandling, thus posing an everlasting ecological threat. Environmental pollution due to the unscrupulous consumption of synthetic polymers derived from petroleum has an adverse impact on the environment since the majority of plastics do not degrade, and the further incineration of synthetic plastics generates CO2 and dioxin. This requires understanding the interactions between materials and microorganisms and the biochemical changes involved. Widespread studies on the biodegradation of plastics have been carried out in order to overcome the environmental problems associated with synthetic plastic waste. Awareness of the waste problem and its impact on the environment has awakened new interest in the area of degradable polymers through microbes viz., bacteria, fungi, and actinomycetes. The microbial degradation of plastics is caused by certain enzymatic activities that lead to a chain cleavage of polymers into oligomers and monomers. This review focuses on the biodegradation rate of plastics by fungal and bacterial communities and the mode of action of biodegradation.

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Section: Biodegradationmentioning

confidence: 99%

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

et al. 2022

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“…PHA biosynthesis systems have several advantages over ROP. PHAs are produced via a one-step fermentation process from different feedstock [ 9 , 10 ]. PHA synthases possess strict enantio-specificity and synthesize isotactic polymers [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR

et al. 2022

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Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaCAR, a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaCAR synthesizes both a block copolymer poly(2-hydroxybutyrate)-b-poly(3-hydroxybutyrate) [P(2HB)-b-P(3HB)], and a random copolymer, poly(3HB-co-3-hydroxyhexanoate), indicating that the combination of monomers determines the monomer sequence. Therefore, in this study, we explored the substrate scope of PhaCAR and the monomer sequences of the resulting copolymers to identify the determinants of the monomer sequence. PhaCAR is a class I PHA synthase that is thought to incorporate long-main-chain hydroxyalkanoates (LMC HAs, > C3 in the main [backbone] chain). Thus, the LMC monomers, 4-hydroxy-2-methylbutyrate (4H2MB), 5-hydroxyvalerate (5HV), and 6-hydroxyhexanoate (6HHx), as well as 2HB, 3HB, and 3-hydroxypropionate (3HP) were tested. Results Recombinant Escherichia coli harboring PhaCAR, CoA transferase and CoA ligase genes was used for PHA production. The medium contained the monomer precursors, 2HB, 3HB, 3HP, 4H2MB, 5HV, and 6HHx, either individually or in combination. As a result, homopolymers were obtained only for 3HB and 3HP. Moreover, 3HB and 3HP were randomly copolymerized by PhaCAR. 3HB-based binary copolymers P(3HB-co-LMC HA)s containing up to 2.9 mol% 4H2MB, 4.8 mol% 5HV, or 1.8 mol% 6HHx were produced. Differential scanning calorimetry analysis of the copolymers indicated that P(3HB-co-LMC HA)s had a random sequence. In contrast, combining 3HP and 2HB induced the synthesis of P(3HP)-b-P(2HB). Similarly, P(2HB) segment-containing block copolymers P(3HB-co-LMC HA)-b-P(2HB)s were synthesized. Binary copolymers of LMC HAs and 2HB were not obtained, indicating that the 3HB or 3HP unit is essential to the polymer synthesis. Conclusion PhaCAR possesses a wide substrate scope towards 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates. 3HB or 3HP units are essential for polymer synthesis using PhaCAR. The presence of a 2HB monomer is key to synthesizing block copolymers, such as P(3HP)-b-P(2HB) and P(3HB-co-LMC HA)-b-P(2HB)s. The copolymers that did not contain 2HB units had a random sequence. This study’s results provide insights into the mechanism of sequence regulation by PhaCAR and pave the way for designing PHA block copolymers.

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“…The price of the carbon substrate used in the PHA production process is a major reason, contributing up to 50% of the total production cost [ 11 , 12 ]. Hence, the utilization of inexpensive and renewable carbon substrates such as agricultural or industrial residues is offering a main solution for reducing PHA production cost [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] .…”

Section: Introductionmentioning

confidence: 99%

A fermentation process for the production of poly(3-hydroxybutyrate) using waste cooking oil or waste fish oil as inexpensive carbon substrate

Loan

Trang

Huy³

et al. 2022

Biotechnology Reports

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Highlights Both WCO and WFO can be used as promising substrates for PHA production. First report of a fed-batch fermentation process using WFO as sole carbon source for PHA production. High PHB yields of 0.8 g/g and 0.92 g/g were produced from WCO and WFO, respectively. Highest PHB productivity (1.73 g/L/h) was achieved when using waste oil as carbon source.

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What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

Cited by 15 publications

References 113 publications

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR

A fermentation process for the production of poly(3-hydroxybutyrate) using waste cooking oil or waste fish oil as inexpensive carbon substrate

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