Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system

Abstract

Gynura procumbens (Lour.) Merris one of medicinal plant which was carried out used as antioxidant, anticancer, anti-inflammatory, hepatoprotective, and antimicrobial. Many strategies were used to increase the production of biomass and valuable compounds. This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of G. procumbens shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2 mg/L and BA 4, 6, 8 mg/L and immersion frequency (5 min each 3 h; 15 min each 12 h). Results showed that the growths of G. procumbens shoots in solid MS medium were influenced by supplementation of growth regulators. MS medium supplemented with single cytokinine (6 mg/L kinetin) and combination of auxin (IAA) and cytokinine (BA) caused increasing of shoots growth. Production of biomass of G. procumbens in temporary immersion bioreactor was achieved in long immersion interval (12 h) and highest flavonoid production was obtained in combination treatment of immersion frequency 15 min each 12 h and MS medium supplemented with IAA 2 mg/L, BA 8 mg/L.

Micropropagation of iraca palm ( Carludovica palmata Ruiz y Pav)using a temporary immersion system

“…2020) who achieve increased shoot length, roots, number of leaves and dry weight with prolonged frequency (8 h), when compared to a short frequency (4 h) in myrtle (M. communis). However, these frequencies are not always optimal to achieve a higher number of shoots and roots per explant (Arano et al 2020;Pramita et al 2018), concomitant with what was reported in our study. A possible explanation for the increase in shoot and root length with prolonged frequencies is that plants respond to water stress with the synthesis of abscisic acid, which is synthesized mainly in roots and leaves (Parkash y Singh, 2020).…”

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

“…Castilleja tenuiflora shoots from an immersion cycle of 30 min every 24 h showed the highest total phenolics (30.58 mg GAE/g DW) and total flavonoid content (45.83 µg CE/g DW) [118], while a lower immersion frequency of 15 min every 12 h increased only the flavonoid (32.00 mg catechin equivalent-CE/g DW) in shoot culture of Gynura procumbens [125]. In Rosa canina plantlets, two immersions per day (15 min/12 h) led to larger content of phenolic compounds (15.8 mg/g FW), whereas more immersions (15 min/6 h) produced a higher accumulation of soluble sugars (9.2 mg/g FW; [132]).…”

Cytokinins: Wide-Spread Signaling Hormones from Plants to Humans with High Medical Potential

“…Additionally, BA was observed to increase the growth of shoots in Gynura procumbens (Lour.) Merr, which is a medicinal plant that has antioxidant, anticancer, anti-inflammatory, hepatoprotective, and antimicrobial activities [ 74 ]. Furthermore, BA was found to improve the antioxidant enzyme activities and total antioxidant capacity and maintain higher levels of ATP content and energy charge in plants [ 75 ].…”