Production of Biologics from Animal Cell Cultures

Warnock, James N.; Al‐Rubeai, Mohamed

doi:10.1007/1-4020-3363-x_24

Cited by 7 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hollow-fiber systems are associated with several disadvantages including poor cell viability, large diffusion gradients, which limit scale-up and effect product quality, and difficulties in culturing anchorage dependent cell lines (Warnock and Al-Rubeai 2005). As medium enters the lumen it encounters a positive transmembrane pressure and is able to permeate into the extra-cellular space.…”

Section: Bioreactor Designmentioning

confidence: 99%

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

2006

Self Cite

View full text Add to dashboard Cite

Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.

show abstract

Section: Bioreactor Designmentioning

confidence: 99%

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

2006

Self Cite

View full text Add to dashboard Cite

show abstract

Cell Immobilization: Engineering Aspects

Willaert

2009

Encyclopedia of Industrial Biotechnology

View full text Add to dashboard Cite

The performance of immobilized cells is influenced by mass transport of substrates to the cells and of products from the cells to the bulk fluid, as well as the cell growth in the immobilization matrix. Both internal and external mass transfer limitations have to be considered in the design of immobilized cell systems. In this article, the various methods of measurement of the diffusion coefficient are reviewed. The influence of the presence and growth of living cells in the immobilization matrix on the diffusion coefficient is discussed. Internal diffusion in gel systems, microcapsules, and dense cell masses, that is biofilms, bioflocs, and mammalian cell aggregates is treated in detail, as well as internal mass transport by convection is considered. Finally, the mathematical modeling of reaction–diffusion systems of immobilized cells is discussed, with special attention to gel entrapment and microencapsulation.

show abstract

Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation

Fraser¹,

Endres²

2013

Disposable Bioreactors II

View full text Add to dashboard Cite

Production of Biologics from Animal Cell Cultures

Cited by 7 publications

References 55 publications

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

Cell Immobilization: Engineering Aspects

Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation

Contact Info

Product

Resources

About