Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation

Fraser, Sheena Janet; Endres, Christian

doi:10.1007/10_2013_238

Cited by 5 publications

(4 citation statements)

References 65 publications

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“…It operates by continuously removing media from the reactor whilst the cells are either retained or returned to the bioreactor allowing higher cell densities due to optimal nutrient conditions and the removal of toxic by-products. Key advantages are higher biomass yield, smaller batches, easier product purification, numbering-up and higher productivity whilst issues are fouling, plugging, higher media requirements and more complex operation [87] and how changes in scale-up affect the cells processes. Fraser and Endres [87] list a range of classic perfusion reactor systems which include membrane reactors, hollow fibre reactors and fixed and rotating bed reactors.…”

Section: Perfusion Processesmentioning

confidence: 99%

“…Key advantages are higher biomass yield, smaller batches, easier product purification, numbering-up and higher productivity whilst issues are fouling, plugging, higher media requirements and more complex operation [87] and how changes in scale-up affect the cells processes. Fraser and Endres [87] list a range of classic perfusion reactor systems which include membrane reactors, hollow fibre reactors and fixed and rotating bed reactors. Fig.…”

Section: Perfusion Processesmentioning

confidence: 99%

See 1 more Smart Citation

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Boodhoo

Flickinger

Woodley

et al. 2022

Chemical Engineering and Processing - Process Intensification

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Section: Perfusion Processesmentioning

confidence: 99%

Section: Perfusion Processesmentioning

confidence: 99%

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Boodhoo

Flickinger

Woodley

et al. 2022

Chemical Engineering and Processing - Process Intensification

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“…They provide a low shear environment, do not need the addition of antifoam agents and give similar or better results than in stirred reusable bioreactors as shown for A. niger, N. crassa and Penicillium spp. [ 87 ].…”

Section: Future Important Technologies For Filamentous Fungimentioning

confidence: 99%

Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper

Meyer¹,

Andersen

Brakhage

et al. 2016

Fungal Biol Biotechnol

225

201

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The EUROFUNG network is a virtual centre of multidisciplinary expertise in the field of fungal biotechnology. The first academic-industry Think Tank was hosted by EUROFUNG to summarise the state of the art and future challenges in fungal biology and biotechnology in the coming decade. Currently, fungal cell factories are important for bulk manufacturing of organic acids, proteins, enzymes, secondary metabolites and active pharmaceutical ingredients in white and red biotechnology. In contrast, fungal pathogens of humans kill more people than malaria or tuberculosis. Fungi are significantly impacting on global food security, damaging global crop production, causing disease in domesticated animals, and spoiling an estimated 10 % of harvested crops. A number of challenges now need to be addressed to improve our strategies to control fungal pathogenicity and to optimise the use of fungi as sources for novel compounds and as cell factories for large scale manufacture of bio-based products. This white paper reports on the discussions of the Think Tank meeting and the suggestions made for moving fungal bio(techno)logy forward.

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“…To bridge the gap between the micrometer scale (nL to μL) of microfluidic chips and the lab-scale (100 mL to L) or even industrial size bioreactors (L to hL), perfusion bioreactors (mL to 100 mL) culturing cells in hollow-fiber cartridges could also be used (Li et al, 2009 ; Whitford and Cadwell, 2009 ; Bonham-Carter and Shevitz, 2011 ; Langer, 2011 ; Shevitz et al, 2011 ; Fraser and Endres, 2013 ; Langer and Rader, 2014 ). Besides being cheap and easily scalable, those hollow-fiber cartridges offer the modularity required for SLMC.…”

Section: Engineering Spatially Linked Microbial Consortia (Slmc)mentioning

confidence: 99%

Synthetic Microbial Ecology: Engineering Habitats for Modular Consortia

Said

2017

Front. Microbiol.

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The metabolic diversity present in microbial communities enables cooperation toward accomplishing more complex tasks than possible by a single organism. Members of a consortium communicate by exchanging metabolites or signals that allow them to coordinate their activity through division of labor. In contrast with monocultures, evidence suggests that microbial consortia self-organize to form spatial patterns, such as observed in biofilms or in soil aggregates, that enable them to respond to gradient, to improve resource interception and to exchange metabolites more effectively. Current biotechnological applications of microorganisms remain rudimentary, often relying on genetically engineered monocultures (e.g., pharmaceuticals) or mixed-cultures of partially known composition (e.g., wastewater treatment), yet the vast potential of “microbial ecological power” observed in most natural environments, remains largely underused. In line with the Unified Microbiome Initiative (UMI) which aims to “discover and advance tools to understand and harness the capabilities of Earth's microbial ecosystems,” we propose in this concept paper to capitalize on ecological insights into the spatial and modular design of interlinked microbial consortia that would overcome limitations of natural systems and attempt to optimize the functionality of the members and the performance of the engineered consortium. The topology of the spatial connections linking the various members and the regulated fluxes of media between those modules, while representing a major engineering challenge, would allow the microbial species to interact. The modularity of such spatially linked microbial consortia (SLMC) could facilitate the design of scalable bioprocesses that can be incorporated as parts of a larger biochemical network. By reducing the need for a compatible growth environment for all species simultaneously, SLMC will dramatically expand the range of possible combinations of microorganisms and their potential applications. We briefly review existing tools to engineer such assemblies and optimize potential benefits resulting from the collective activity of their members. Prospective microbial consortia and proposed spatial configurations will be illustrated and preliminary calculations highlighting the advantages of SLMC over co-cultures will be presented, followed by a discussion of challenges and opportunities for moving forward with some designs.

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Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation

Cited by 5 publications

References 65 publications

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper

Synthetic Microbial Ecology: Engineering Habitats for Modular Consortia

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