Production of biologically active Atlantic salmon interferon in transgenic potato and rice plants

Fukuzawa, Noriho; Tabayashi, Noriko; Okinaka, Yasushi; Furusawa, Ryo; Furuta, Kazuyoshi; Kagaya, Uiko; Matsumura, Takeshi

doi:10.1016/j.jbiosc.2010.02.005

Cited by 24 publications

(5 citation statements)

References 38 publications

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“…For the production of AMP substances, the application of a plant bioreactor can effectively prevent the destruction of the host cells by the target products, thus demonstrating its unique advantages (Huang et al ; Yang et al ; Fukuzawa et al ). For the first time, we report an attempt to use an AMP from shrimp produced by a plant bioreactor to control diseases in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

Liu

Wang

Jiang

et al. 2014

J World Aquaculture Soc

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Penaeidin is an antimicrobial peptide family found in penaeid shrimp. Penaeidin3 has a broad spectrum of antimicrobial activity and is most effective against certain bacterial species. In this study, the penaeidin3‐2 gene was cloned from the Chinese shrimp, Fenneropenaeus chinensis. It was then transformed into rice plants using an Agrobacterium‐mediated transformation system. The transformants were verified by polymerase chain reaction (PCR) sequencing and real‐time polymerase chain reaction (RT‐PCR) analysis. To evaluate the application of penaeidin3‐2 expressed by rice plants in the feedstock industry and aquaculture practice, the transgenic rice bran was added to fish feed. The proliferation of microbes in the feed was significantly inhibited by the transgenic rice bran. Furthermore, the mortality of tilapias challenged with Aeromonas hydrophila was also significantly reduced when they were fed with the transgenic bran expressing penaeidin3‐2. Their intestinal flora changed when the fish were fed with rice bran expressing the introduced gene for a relatively long period. Fish that survived the challenge had a more intact midgut structure. These results indicate that penaeidin3‐2 expressed in rice plants may possess the ability to modulate the intestinal bacterial community and protect the gut structure from damage.

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Section: Discussionmentioning

confidence: 99%

Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

Liu

Wang

Jiang

et al. 2014

J World Aquaculture Soc

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“…Cholera toxin B subunit protein was expressed in potato tubers and was able to induce serum and intestinal anti-CTB antibodies [154,155]. Transgenic potato tubers have also been used to produce viral antigens such as hepatitis B surface antigen [156,157], Norwalk virus coat protein [158,159], epitope of porcine epidemic diarrhea virus [160], GP5 protein of Porcine reproductive and respiratory syndrome virus [161], Rotavirus VP7 [162], infectious bronchitis virus (IBV) S1 glycoprotein [163], and human papilloma virus major capsid protein L1 [164,165] or oncogene E7 [166] as oral vaccine candidates. Yu and Langridge [167], constructed a combination of Cholera toxin B and A2 subunit, rotavirus enterotoxin and enterotoxigenic E. coli fimbrial antigen genes and were expressed in transgenic potatoes.…”

Section: Heterologous Protein Production In Roots/tubersmentioning

confidence: 99%

“…In another study, ricin subunit B from castor bean was used as an immunomodulatory molecule capable of enhancing immunosuppression associated with Type I diabetes [171]. Subtypes of human and salmon interferon alpha was produced in transgenic potatoes and effectively tested for their bioactivity against viral strains [156][157][158]. To increase the nutritional quality of potato tubers, AmA1 gene from Amaranthus hypochondriacus was transformed into potato plants in a tuber specific manner.…”

Section: Heterologous Protein Production In Roots/tubersmentioning

confidence: 99%

Plant Platforms for Efficient Heterologous Protein Production

Ghag

Adki

Ganapathi

et al. 2021

Biotechnol Bioproc E

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Production of recombinant proteins is primarily established in cultures of mammalian, insect and bacterial cells. Concurrently, concept of using plants to produce high-value pharmaceuticals such as vaccines, antibodies, and dietary proteins have received worldwide attention. Newer technologies for plant transformation such as plastid engineering, agroinfiltration, magnifection, and deconstructed viral vectors have been used to enhance the protein production in plants along with the inherent advantage of speed, scale, and cost of production in plant systems. Production of therapeutic proteins in plants has now a more pragmatic approach when several plant-produced vaccines and antibodies successfully completed Phase I clinical trials in humans and were further scheduled for regulatory approvals to manufacture clinical grade products on a large scale which are safe, efficacious, and meet the quality standards. The main thrust of this review is to summarize the data accumulated over the last two decades and recent development and achievements of the plant derived therapeutics. It also attempts to discuss different strategies employed to increase the production so as to make plants more competitive with the established production systems in this industry.

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“…The first reports about the plan-based production of human interferon date back to the beginning of the 90 s [56], however it was the usage of transplanstomic plants that allowed for the huge increase of yield, up to 20% of TSP [45]. It is worth mentioning that not only human interferons but also interferons from chicken [49] and salmon [50] were successfully produced in plant-based systems.…”

Section: Recombinant Cytokines From Plant-based Platformsmentioning

confidence: 99%

Recombinant Cytokines from Plants

Sirko

Vanek

Góra-Sochacka

et al. 2011

IJMS

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Plant-based platforms have been successfully applied for the last two decades for the efficient production of pharmaceutical proteins. The number of commercialized products biomanufactured in plants is, however, rather discouraging. Cytokines are small glycosylated polypeptides used in the treatment of cancer, immune disorders and various other related diseases. Because the clinical use of cytokines is limited by high production costs they are good candidates for plant-made pharmaceuticals. Several research groups explored the possibilities of cost-effective production of animal cytokines in plant systems. This review summarizes recent advances in this field.

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Production of biologically active Atlantic salmon interferon in transgenic potato and rice plants

Cited by 24 publications

References 38 publications

Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

Plant Platforms for Efficient Heterologous Protein Production

Recombinant Cytokines from Plants

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