Production of Biodiesel Using Liquid Lipase Formulations

Nielsen, Per Henning; Rancke‐Madsen, Anders; Holm, Hans; Burton, Rachel

doi:10.1007/s11746-016-2843-4

Cited by 61 publications

(35 citation statements)

References 13 publications

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“…The enzyme being an amphiphilic molecule acting on the interface water/oil, remains concentrated, after separation by gravity, in an emulsion between a superior layer (rich in FAAE) and a clear inferior layer (rich in glycerol and water). Nielsen et al [112] relate that more than 90% of the enzyme activity is located in this emulsion phase. However, considering the fact that the lipase recuperation may be problematic depending on the raw material utilized in the process and considering that recent advances reduced the price of the enzyme at a level where a unique use of the biocatalyst is feasible, the lipase reutilization becomes unnecessary [28,112].…”

Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.

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Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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show abstract

“…[26] For each cycle, the soluble lipase was recovered from the reaction mixture and was directly utilized in the next batch without any treatment. It is possible to recover the liquid enzyme for further reutilization with immobilized enzymes, which is another economic advantage of soluble lipases.…”

Section: Resultsmentioning

confidence: 99%

Feeding strategies of methanol and lipase on eversa® transform‐mediated hydroesterification for FAME production

et al. 2019

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This work reports the influence of different feeding strategies of methanol and catalysts on fatty acid methyl esters (FAME) yield in a beef tallow hydroesterification reaction for biodiesel production catalyzed by liquid lipase. Soluble lipase from the Thermomyces lanuginosus microorganism, named Eversa 1 Transform, was selected for the reactions in this work. The feeding of methanol and lipase into the system was evaluated varying the methanol to fat molar ratio of 4.0:1 and 4.5:1 and the lipase load of 1.0 wt% and 1.45 wt% in relation to the mass of beef tallow utilized in each assay. The highest yield (85.08 %) of FAME was reached at 35 8C, methanol to fat molar ratio of 4.5:1, 1.0 wt% of lipase, 6.0 wt% of water in only 8 h of reaction, with a one-step addition of lipase and methanol fed to the process at a constant flow of 3.0 g Á h À1 . Moreover, the reutilization process of the liquid lipase after four cycles was evaluated, which is a point rarely addressed in similar works available in the open literature. The findings of this paper demonstrate that the adoption of an appropriate strategy to feed inputs to the system is fundamental in order to take advantage of the maximum catalytic capacity of the lipase and consequently to obtain high yields in the process.

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“…Posteriormente, as amostras de óleo com e sem tratamento térmico foram caracterizadas por espectroscopia no infravermelho com Transformada de Fourier (FTIR) com Reflectância Total Atenuada (ATR). A reação de transesterificação foi realizada com base no artigo de Nielsen et al (2016), a qual se deu à temperatura ambiente, 0,7% m/m de catalisador base biomassa, 4% de água, razão molar metanol:óleo 3:1, sendo que 20% da massa do catalisador iniciou na reação e o restante foi adicionado numa vazão de 1 mL.h -1 e tempo de reação de 24 h. A produção do biodiesel, assim como as análises de massa específica (por picnometria), acidez (por titulação) e FTIR-ATR, foram realizadas em triplicatas.…”

Section: Materiais E Métodosunclassified

Utilização De Espectroscopia Na Região Do Infravermelho Para Avaliar a Reação De Transesterificação De Amostras De Triglicerídeos Após Tratamento Térmico.

Godoy¹,

Temoteo²,

Silva³

et al. 2018

Blucher Chemical Engineering Proceedings

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RESUMO -Este trabalho teve como objetivo avaliar a síntese de biodiesel por transesterificação enzimática pela enzima Eversa® Transform de amostras de óleo comercial termicamente tratado. O óleo comercial foi submetido a tratamento térmico a 160 °C. Foram avaliados os parâmetros massa específica (ME), índice de acidez (IA) e foi realizada Espectroscopia no Infravermelho com Transformada de Fourier e Reflectância Total Atenuada FTIR-ATR. O tratamento térmico não afetou (p=0,05) a massa específica e o perfil de FTIR-ATR dos produtos obtidos a partir dos óleos. Estes produtos foram confirmados como biodiesel pelo surgimento de bandas a 1017 e 1437 cm -1 no espectro vibracional. A análise por FTIR-ATR evidenciou sobreposições de bandas, confirmadas pela ausência do aumento de intensidade na banda 966 cm -1 (isômero trans). Entretanto, os valores médios do índice de acidez apresentaram diferenças superiores a 12% para o óleo tratado, embora todos os parâmetros analisados tenham alcançado resultados médios dentro das especificações da ANP para ambas as amostras. Dessa forma pode-se concluir que a enzima Eversa Transform pode ser usada potencialmente na biocatálise para a produção de biodiesel, fazendo uso de óleos submetidos a altas temperaturas.

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Production of Biodiesel Using Liquid Lipase Formulations

Cited by 61 publications

References 13 publications

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipases in liquid formulation for biodiesel production: Current status and challenges

Feeding strategies of methanol and lipase on eversa® transform‐mediated hydroesterification for FAME production

Utilização De Espectroscopia Na Região Do Infravermelho Para Avaliar a Reação De Transesterificação De Amostras De Triglicerídeos Após Tratamento Térmico.

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