Production of bioactive compounds and gene expression alterations in hairy root cultures of chinese cabbage elicited by copper oxide nanoparticles

Chung, Ill‐Min; Rekha, Kaliyaperumal; Rajakumar, Govindasamy; Thiruvengadam, Muthu

doi:10.1007/s11240-018-1402-0

Cited by 48 publications

(31 citation statements)

References 41 publications

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“…Elicitation with Fe NPs raised the TPC (4.65 mg g −1 DW) and TFC (77.34 μg g −1 DW) in the hairy roots of Cichorium intybus . The TPC and TFC accumulation was prominently induced by the elicitation of copper oxide nanoparticles in the hairy roots of Brassica rapa and silver nanoparticles in Cucumis anguria …”

Section: Resultsmentioning

confidence: 99%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects and with antiproliferative potential against various cancer cells. The extensive application of nanotechnology in hairy root cultures is a new sustainable production platform for producing these active constituents. In the present study, hairy roots derived from 4‐week‐old leaves and Agrobacterium rhizogenes strain ATCC15834 were used to investigate the impact of various concentrations of iron oxide nanoparticles (Fe NPs) in two elicitation time exposures (24 and 48 h) on growth, antioxidant enzyme activity, total phenolic and flavonoid content (TPC and TFC), and some polyphenols. Gene expression levels of phenylalanine ammonia‐lyase (pal) and rosmarinic acid synthase (ras) were also analyzed. RESULTS Iron nanoparticles enhanced biomass accumulation in hairy roots. The treatment time and Fe NP dosage largely improved the activity of antioxidant enzymes, TPC and TFC. The highest RA (1194 μg g−1 FW) content (9.7‐fold), compared to controls, was detected with 24 h of exposure to 75 mg L−1 Fe NP, which was consistent with the expression of pal and ras genes under the influence of elicitation. The xanthomicrol, cirsimaritin, and isokaempferide content was increased 11.87, 3.85, and 2.27‐fold, respectively. CONCLUSION Stimulation of D. kotschyi hairy roots by Fe NPs led to a significant increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and xanthomicrol. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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“…CuO NP treated and non-treated CSC extracts were examined for their antimicrobial activity as previously described [25,29]. Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), and fungi (Aspergillus niger, Fusarium oxysporum, and Candida albicans) were used for the disc diffusion method [25,29].…”

Section: Antibacterial and Antifungal Activitiesmentioning

confidence: 99%

“…The Cu level was higher (1.1 mg/L) in 5 mg/L CuO NP treated CSC compared with the value in 1 or 3 mg/L of CuO NP treated CSC (Figure 2a). Similarly, Cu accumulation in B. rapa was reportedly enhanced depending on treatment with CuO NPs [29]. A high concentration of Cu ions may be toxic; for example, binding of Cu to sulfhydryl groups of proteins can inhibit enzyme activities and may induce a deficiency of other elements through competition with ions like iron or by imposing oxidative stress in plant cells [39].…”

Section: Biomass Cu and Ga II Content In Cuo Np Treated And Non-trementioning

confidence: 99%

“…Several studies reported that the inclusion of an abiotic or biotic elicitor to the growth medium notably elevated the synthesis of bioactive compounds by activating a defense system [29]. We investigated the impact of CuO NPs on the accumulation of TPC and TFC in CSC of G. sylvestre.…”

Section: Tpc and Tfc In Cscmentioning

confidence: 99%

“…However, the use of 5 mg/L of CuO NPs in CSC decreased the amount of TPC (190.15 GAE) and TFC (3.85 QE). TPC and TFC were improved by the elicitation of CuO NPs in hairy roots of Chinese cabbage [29] and AgNPs in CSC of bitter gourd [25]. Elicitation with silicon dioxide NPs elevates the PC accumulation in hairy roots of Dracocephalum kotschyi [45].…”

Section: Tpc and Tfc In Cscmentioning

confidence: 99%

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Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

et al. 2019

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Gymnema sylvestre is a plant that is enriched in bioactive compounds. In particular, gymnemic acids (GA) and phenolic compounds (PC) are pharmaceutically important. There is a commercial demand for naturally occurring bioactive compounds, but their availability is limited due to geographical and seasonal variations. The elicitation approach can enhance the biosynthesis of phytochemicals during in vitro culture of G. sylvestre. Here, to further improve gymnemic acid II (GA II) and phenolic compounds (PC) production by G. sylvestre, cell suspension cultures (CSC), which has attracted attention for the production of essential phytochemicals, was explored using copper oxide nanoparticles (CuO NPs). Callus was obtained on MS medium containing 2,4-dichlorophenoxyacetic acid, kinetin, phytoagar, and sucrose. Agar-free MS medium was used to initiate CSC, which was treated with three concentrations of CuO NPs (1, 3 or 5 mg/L). Treatment for 48 h with 3 mg/L CuO NPs resulted in the greatest yields of GA II, total phenolics, and flavonoids. The cultures also displayed pronounced antioxidant, antidiabetic, anti-inflammatory, antibacterial, antifungal, and anticancer activities. The use of CuO NPs (3 mg/L) significantly increased the production of GA II (nine-fold) and PC compared to unamended CSC. We propose that CSC and use of nanoparticles (NPs) as a new generation of elicitors, offer a suitable prospect for the production of bioactive compounds.

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Designer nanoparticles for plant cell culture systems: Mechanisms of elicitation and harnessing of specialized metabolites

et al. 2021

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Plant cell culture systems have become an attractive and sustainable approach to produce high-value and commercially significant metabolites under controlled conditions. Strategies involving elicitor supplementation into plant cell culture media are employed to mimic natural conditions for increasing the metabolite yield. Studies on nanoparticles (NPs) that have investigated elicitation of specialized metabolism have shown the potential of NPs to be a substitute for biotic elicitors such as phytohormones and microbial extracts. Customizable physicochemical characteristics allow the design of monodispersed-, stimulus-responsive-, and hormone-carrying-NPs of precise geometries to enhance their elicitation capabilities based on target metabolite/plant cell culture type. We contextualize advances in NP-mediated elicitation, especially stimulation of specialized metabolic pathways, the underlying mechanisms, impacts on gene regulation, and NP-associated cytotoxicity. The novelty of the concept lies in unleashing the potential of designer NPs to enhance yield, harness metabolites, and transform nanoelicitation from exploratory investigations to a commercially viable strategy.

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Production of bioactive compounds and gene expression alterations in hairy root cultures of chinese cabbage elicited by copper oxide nanoparticles

Cited by 48 publications

References 41 publications

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

Designer nanoparticles for plant cell culture systems: Mechanisms of elicitation and harnessing of specialized metabolites

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