Production of arabinoxylan-oligosaccharide mixtures of varying composition from rye bran by a combination of process conditions and type of xylanase

Falck, P; Aronsson, Anna; Grey, Carl; Stålbrand, Henrik; Karlsson, Eva Nordberg; Adlercreutz, Patrick

doi:10.1016/j.biortech.2014.09.139

Cited by 51 publications

(27 citation statements)

References 35 publications

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“…In addition, arabinoxylan from rye, purified as described in Falck et al . , was used as substrate. The reaction mix consisted of 1 m m of oligosaccharide, 50 m m CPB pH 5.5, and 2–4 μg·mL −1 of enzyme.…”

Section: Methodsmentioning

confidence: 99%

“…The following compounds were purchased from Megazyme (Wicklow, Ireland): 1,5-a-L-arabinobiose, 1,5-a-L-arabinotriose, 1,3-a-L-arabino-b-D-xylobiose, and debranched arabinan (sugar beet). In addition, arabinoxylan from rye, purified as described in Falck et al [39], was used as substrate. The reaction mix consisted of 1 mM of oligosaccharide, 50 mM CPB pH 5.5, and 2-4 lgÁmL À1 of enzyme.…”

Section: Specific Activity For Oligosaccharides and Polysaccharidesmentioning

confidence: 99%

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Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

et al. 2017

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Arabinofuranosidases degrade arabinose-containing oligo and polysaccharides, releasing L-arabinose, which is a potentially useful sugar, shown to reduce glycemic response under certain conditions. Arabinofuranosidases (Arafs) are frequently found in GH43, one of the most common GHfamilies encoded in genomes in gut microbiota, and hence it is of interest to increase understanding of the function of these enzymes in species occurring in the gut. Here we have produced, characterized and solved the three-dimensional structures, at 1.9 and 2.0 A resolution respectively, of two homologous GH43 enzymes, classified under subfamily 26, from Lactobacillus brevis DSM1269 (LbAraf43) and Weissella strain 142 (WAraf43), respectively. The enzymes, with 74% sequence identity to each other, are composed of a single catalytic module with a b-propeller structure typical of GH43, and an active-site pocket with three identifiable subsites (À1, +1, and +2). According to size exclusion chromatography, native WAraf43 is a dimer, while LbAraf43 is a tetramer in solution. Both of them show activity with similar catalytic efficiency on 1,5-a-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3. Activity is restricted to substrates of low DP, and the reason for this is believed to be an extended loop at the entrance to the active site, creating interactions in the +2 subsite.

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Section: Methodsmentioning

confidence: 99%

Section: Specific Activity For Oligosaccharides and Polysaccharidesmentioning

confidence: 99%

Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

et al. 2017

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“…It was suggested that the fibre combination might reduce the low-grade inflammatory response and prevent the risk of cardiovascular disease (Johansson-Persson et al, 2014). Heat pretreatment and enzymatic hydrolysis of rye bran yielded arabinoxylanoligosaccharides with functional food potential (Falck et al, 2014). …”

Section: Rye (Secale Cereale)mentioning

confidence: 99%

Cereal bran fortified-functional foods for obesity and diabetes management: Triumphs, hurdles and possibilities

Patel

2015

Journal of Functional Foods

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“…In order to increase the AX extraction rate or the yield of AXOS, xylanases usually worked in the optimum conditions, which was quite different from the processing conditions of CSB. AXOS yields reached 62% after the treatment of the xylanase from Neocallimastix patriciarum for 24 h while the xylanases contributed to the solubilisation of dietary fibre from wheat and rye bran at 50 °C …”

Section: Introductionmentioning

confidence: 99%

The effect of pentosanase on the solubilisation and degradation of arabinoxylan extracted from whole and refined wheat flour

et al. 2016

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Production of arabinoxylan-oligosaccharide mixtures of varying composition from rye bran by a combination of process conditions and type of xylanase

Cited by 51 publications

References 35 publications

Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

Three‐dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis

Cereal bran fortified-functional foods for obesity and diabetes management: Triumphs, hurdles and possibilities

The effect of pentosanase on the solubilisation and degradation of arabinoxylan extracted from whole and refined wheat flour

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