Production of Antiserum to LH-Releasing Hormone (LH-RH) Associated with Gonadal Atrophy in Rabbits: Development of Radioimmunoassays for LH-RH

Arimura, Akira; Satô, Hiroshi; Kumasaka, T; Worobec, R.B.; Debéljuk, Luciano; Dunn, Jon D.; Schally, Andrew V.

doi:10.1210/endo-93-5-1092

Cited by 193 publications

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“…These results revealed the molecular multiplicity of LHRH. Many kinds of polyclonal anti-LHRH sera have been prepared and contributed to the studies on LHRH (Arimura et al, 1973(Arimura et al, , 1975Copeland et al, 1979), however, they could be used only for limited species because of strict species specificity, and also could not detect possible precursors because of the special structure of LHRH in the Nand C-terminals. Recently, monoclonal antibodies which were prepared by hybridoma technology (Kohler and Milstein, 1975) with highly specific binding sites, have been used widely as immunochemical probes.…”

mentioning

confidence: 99%

Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

1986

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confidence: 99%

Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

1986

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“…In order to evaluate the effect of these analogues on the diseases referred to, sensitive determination methods for these analogues in biological media are essential. However, there have been few reports of the radioimmunoassay of these analogues, a similar method being established as the most sensitive one to determiae the LH-RH (Arimura et al, 1973;Nett et al, 1973;Kinugasa et al, 1976;Copeland et al, 1979). Fractions shadowed were used for radioimmunoassay.…”

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confidence: 99%

A radioimmunoassay for a highly active luteinizing hormone-releasing hormone analogue and relation between the serum level of the analogue and that of gonadotropin.

Yamazaki

Okada

1980

Endocrinol Japon

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Antisera to an LH-RH analogue, des-Gry10-[D-Leu6]-LH-RH-ethylamide (TAP-144) were produced in 10 rabbits. By using these antisera, a specific and sensitive radioimmunoassay for TAP-144 was established.Sensitivity of the radioimmunoassay ranged from 5 to 100 pg per assay tube with these antisera.LH, FSH, TRH, LH-RH, and the 1-6 fragment of TAP-144 did not practically cross-react with these antisera. However, the antisera showed a tendency to cross-react with LH-RH analogues, though the degree of the cross-reactivity differed among individual sera. The average crossreactivity of the 10 antisera showed low specificities to TAP-144 analogues which are altered at positions 2, 3, 4, 5, and 6, but showed high specificities to those altered at positions 8 and 9. The antisera also showed low cross-reactivities to LH-RH analogues replaced at position 6 by D-amino acids and those altered at position 10 by alkylamines, but they showed fairly high cross-reactivities to analogues which are altered simultaneously at both positions 6 and 10.When TAP-144 was administered intraperitoneally to rats on the diestrous day, serum concentrations of TAP-144 increased dose-dependently but maximal serum concentrations of both LH and FSH were attained in response to higher doses of TAP-144. The peak LH and FSH concentrations appeared 70 to 110 min after the peak TAP-144 concentration had been reached. Similar delays in reaching the peak LH and FSH levels were also observed when TAP-144 was administered intravenously, subcutaneously and intramuscularly.When TAP-144 was administered intravaginally, a low but constant serum level of TAP-144 was maintained from 5 to 300 min after the administration, but serum LH and FSH levels declined to a low level from 180 min after the TAP-144 administration.

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“…This inhibited the pituitary output and reduced the content of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the blood, severely impaired testosterone production and spermatogenesis and caused the testis to involute to 10-20% of the normal weight (Arimura et al, 1973;Fraser & Gunn, 1973;Fraser, Gunn, Jeffcoate & Holland, 1974;. Similar changes have also been observed in GnRH-immunized marmoset monkeys (Hodges & Hearn, 1977) and rhesus monkeys (Chappel, Ellinwood, Huckins, Herbert & Spies, 1980).…”

Section: Gnrh Antibodiesmentioning

confidence: 79%

Antifertility effects of GnRH

Fraser¹

1982

Reproduction

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Production of Antiserum to LH-Releasing Hormone (LH-RH) Associated with Gonadal Atrophy in Rabbits: Development of Radioimmunoassays for LH-RH

Cited by 193 publications

References 0 publications

Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

A radioimmunoassay for a highly active luteinizing hormone-releasing hormone analogue and relation between the serum level of the analogue and that of gonadotropin.

Antifertility effects of GnRH

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