Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

Park, Min Kyun; Wakabayashi, Keiji

doi:10.1507/endocrj1954.33.257

Cited by 103 publications

(52 citation statements)

References 43 publications

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“…Especially with a monoclonal antibody, radioiodination of the tyrosine or histidine which is included in the antigenic determinant causes a serious loss of its antibody binding ability (6). Presence of antibodies to other substances in antiserum…”

Section: Resultsmentioning

confidence: 99%

Assessment of specificity of antisera for immunohistochemistry.

Wakabayashi

Tanaka

1988

Acta Histochem. Cytochem

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Section: Resultsmentioning

confidence: 99%

Assessment of specificity of antisera for immunohistochemistry.

Wakabayashi

Tanaka

1988

Acta Histochem. Cytochem

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“…Thus, for our analysis of hypothalamic GnRH (GnRH1), we used the LRH13 antibody (Park and Wakabayashi, 1986), which recognizes both the terminal nerve and hypothalamic GnRH cell populations. Immunocytochemistry was carried out as previously described (Whitlock et al, 2003), except that the embryos were fixed in 4% paraformaldehyde (Westerfield, 1993) with 7% saturated picric acid for two hours at room temperature.…”

Section: Immunocytochemistrymentioning

confidence: 99%

A role forfoxd3andsox10in the differentiation of gonadotropin-releasing hormone (GnRH) cells in the zebrafishDanio rerio

et al. 2005

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Gonadotropin-releasing hormone (GnRH) is found in a wide range of vertebrate tissues, including the nervous system. In general, GnRH has two functions: endocrine, acting as a releasing hormone; and neuromodulatory,affecting neural activity in the peripheral and central nervous system. The best understood population of GnRH cells is that of the hypothalamus, which is essential for reproduction. Less well understood are the populations of GnRH cells found in the terminal nerve and midbrain, which appear to be neuromodulatory in function. The GnRH-containing cells of the midbrain are proposed to arise from the mesencephalic region of the neural tube. Previously, we showed that neuromodulatory GnRH cells of the terminal nerve arise from cranial neural crest. To test the hypothesis that neuromodulatory GnRH cells of the midbrain also arise from neural crest, we used gene knockdown experiments in zebrafish to disrupt neural crest development. We demonstrate that decrement of the function of foxd3 and/or sox10, two genes important for the development and specification of neural crest, resulted in a reduction and/or loss of GnRH cells of the midbrain, as well as a reduction in the number of terminal nerve GnRH cells. Therefore, our data support a neural crest origin for midbrain GnRH cells. Additionally, we demonstrate that knockdown of kallmann gene function resulted in the loss of endocrine GnRH cells of the hypothalamus, but not of neuromodulatory GnRH cells of the midbrain and terminal nerve, thus providing additional evidence for separate pathways controlling the development of neuromodulatory and endocrine GnRH cells.

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“…This antibody recognizes the region around Ser 4 -Tyr 5 , which is a common amino acid sequence of GnRH in a variety of animal species (Park and Wakabayashi, 1986). The antibody also binds precursor peptides of GnRH, but had less affinity for chicken GnRH-II in a competitive binding experiment (Park and Wakabayashi, 1986).…”

Section: Murakami Et Almentioning

confidence: 99%

Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain

Murakami

Ohki‐Hamazaki

Watanabe

et al. 2010

J of Comparative Neurology

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Hypothalamic gonadotropin-releasing hormone (GnRH) neurons originate in the olfactory placode and migrate to the forebrain during embryonic development. We found that GnRH neurons migrated in two different modes in the chick medial telencephalon: they initially underwent axophilic migration in association with a subset of olfactory fibers in a dorsocaudal direction. This was followed by ventrally directed tangential migration to the basal forebrain. Since many of the ventrally migrating GnRH neurons did not follow distinct fiber fascicles, it is proposed that diffusible guidance molecules played a role in this migratory process. A long-range diffusible factor, netrin 1, was expressed in the lower part of the commissural plate and the subpallial septum, but not along the axophilic migratory route of GnRH neurons. Failure of ventrally directed migration of GnRH neurons and their misrouting to the dorsomedial forebrain was induced by misexpression of netrin 1 in the dorsocaudal part of the septum near the top of the commissural plate, which is where the migration of GnRH neurons changed to a ventral direction. In such cases, a subset of olfactory fibers also extended, but close contact between aberrant fibers and misrouted GnRH neurons did not exist. A coculture experiment demonstrated that netrin 1 exerts an attractive effect on migrating GnRH neurons. These results provide evidence that netrin 1 acts as chemoattractant to migrating GnRH neurons at the dorsocaudal part of the septum and has the potential to regulate the ventral migration of GnRH neurons to the ventral septum and the preoptic area.

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Preparation of a Monoclonal Antibody to Common Amino Acid Sequence of LHRH and Its Application

Abstract: In order to prepare

Cited by 103 publications

References 43 publications

Assessment of specificity of antisera for immunohistochemistry.

Assessment of specificity of antisera for immunohistochemistry.

A role forfoxd3andsox10in the differentiation of gonadotropin-releasing hormone (GnRH) cells in the zebrafishDanio rerio

Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain

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