Production of Antisera for Polypeptide and Steroid Radioimmunoassays

Odell, William D.; Skowsky, R.; Abraham, Guy E.; Hescox, M. A.; Fisher, Delbert A.; Grover, P. K.

doi:10.1093/biolreprod/6.3.427

Cited by 29 publications

(8 citation statements)

References 21 publications

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“…This antiserum, designated R4-B6-1, revealed a K a of approximately 6 X 10 10 litres/mol (19) and was used for the studies reported here. In this rabbit, the antiserum titers continued to increase until the fifth booster dose, at which time this antiserum, at a 1:50,000 final dilution of 0.5 ml, bound 40% of the added (10,000 cpm) [ 125 I]SM.…”

Section: Evaluation Of the Sm Riamentioning

confidence: 99%

Radioimmunoassay of a Basic Somatomedin: Comparison of Various Assay Techniques and Somatomedin Levels in Various Sera*

Bala¹,

Bhaumick²

1979

The Journal of Clinical Endocrinology & Metabolism

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Purification of a basic somatomedin (SM), with similarity to SM-C and insulin-like growth factor, from human plasma Cohn fraction IV-1 enabled development of a RIA based on this SM.SM antiserum was produced by immunizing rabbits with partially purified SM. This antiserum (final dilution, 1:50,000) specifically bound approximately 40% of added [125I]-SM in this RIA. The RIA sensitivity was 2 x 10(-4) U immunoreactive SM (IRSM). Highly purified SM-C, insulin-like growth factor 1, and our SM revealed parallel and approximately equipotent dose-response curves in this RIA; rat SM and multiplication stimulation activity revealed less cross-reactivity. IRSM was detected in sera of all species tested except fish. Acidification of sera, without subsequent chromatography, before assay permitted measurement of total IRSM with either an equilibrium or nonequilibrium RIA technique. Acidification of serum appears to increase SM-binding capacity while decreasing binding affinity of the 20,000--50,000 mol wt proteins in serum. The mean (+/- SEM) IRSM concentrations in sera from normals and patients with acromegaly, hypopituitarism, GH deficiency before/after treatment, and Laron dwarfism were 1.45 +/- 0.17, 5.49 +/- 0.48, 0.19 +/- 0.07, 0.10 +/- 0.02/0.64 +/- 0.45, and 0.25 +/- 0.11 U/ml, respectively, compared to a pooled normal human serum reference standard which was designated to contain 1 IRSM U/ml. Measurements of total IRSM (bound and free) in serum may not accurately reflect SM bioactivity and will require interpretative caution.

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Section: Evaluation Of the Sm Riamentioning

confidence: 99%

Radioimmunoassay of a Basic Somatomedin: Comparison of Various Assay Techniques and Somatomedin Levels in Various Sera*

Bala¹,

Bhaumick²

1979

The Journal of Clinical Endocrinology & Metabolism

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“…Antiscra to AVP and AVT were produced in New Zcaland White female rabbits, using lysinc vasopressin conjugated to bovine thyroglobulin as previously described (12,22). Synthetic AVP ancl AVT a e r c iodinated, using a modification of the method of Hunter ancl Greenwood (24).…”

Section: Speculationmentioning

confidence: 99%

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Fisher

1977

Pediatr Res

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“…Antisera with marked increase in affinity of binding, and therefore sensitivity for hormone measurement, were developed and utilized. Each antiserum was shown to be specific by immunologic studies and by bioassay-immunoassay correlations (39). (These antisera now are being produced on a large scale and are being distributed by NIH.)…”

Section: Subjectsmentioning

confidence: 99%

Luteinizing Hormone, Follicle Stimulating Hormone, and Growth Hormone Secretion in Normal Adult Men During Sleep and Dreaming

Rubín

Gouin

Kales

et al. 1973

Psychosomatic Medicine

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The patterns of LH, FSH and GH release during all-night sleep were studied in 16 normal young adult men, sampled on two consecutive nights. The release of both gonadotropins occurred in random, unrelated peaks that showed no consistency between subjects or from night to night in the same subject. No circadian or ultiadian rhythm was discernible for either LH or FSH. When analyzed by sleep stages, LH levels showed a modest but significant increase (14%) during REM sleep compared to the other stages of sleep. FSH levels, on the other hand, showed no clear relationship to sleep stages. Growth hormone did show the usual marked increase coincident with the onset of slow wave sleep.

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Production of Antisera for Polypeptide and Steroid Radioimmunoassays

Cited by 29 publications

References 21 publications

Radioimmunoassay of a Basic Somatomedin: Comparison of Various Assay Techniques and Somatomedin Levels in Various Sera*

Radioimmunoassay of a Basic Somatomedin: Comparison of Various Assay Techniques and Somatomedin Levels in Various Sera*

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Luteinizing Hormone, Follicle Stimulating Hormone, and Growth Hormone Secretion in Normal Adult Men During Sleep and Dreaming

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