Production of Angiotensin Converting Enzyme by Cultured Bovine Endothelial Cells

Abstract

1. Endothelial cells from bovine aortae were grown to confluence in tissue culture. The cells were subcultured and angiotensin converting enzyme (ACE) production was measured while the cells were maintained in serum-free medium for 4 days. 2. During this period, there was a ten-fold increase in ACE concentration in the medium whereas the cellular content of the enzyme remained constant at approximately 23% of the total enzyme produced. Cycloheximide (1 mumol/l) blocked production of ACE. 3. The enzyme liberated into the medium closely resembled ACE in the following respects: it cleaved the dipeptide histidyl-leucine from a synthetic tripeptide substrate, was markedly Cl- activated and inhibited by EDTA (1 mmol/l), SQ 14225 (1 mumol/l) or SQ 20881 (1 mumol/l). 4. Cells maintained in culture for up to ten passages have maintained morphology typical of endothelial cells and continue to produce ACE.

Effect of Captopril on the bradykinin‐induced release of prostacyclin from guinea‐pig lungs and bovine aortic endothelial cells

“…Alhenc-Galas (1982) also showed that captopril was without effect on prostacyclin production induced by Bk in human cultured endothelial cells. Endothelial cells produce ACE in vitro (Mendelsohn & Kachel, 1981), so that a potentiation of the effect of Bk by captopril might be expected, as seen in the guinea-pig isolated lungs. However, there are many more endothelial cells in a guinea-pig isolated lung (109) than in our column of endothelial cells (107).…”

Induction by Glucocorticoids of Angiotensin Converting Enzyme Production from Bovine Endothelial Cells in Culture and Rat Lung In Vivo

“…Techniques for harvesting and culture of endothelial cells have been described (6), and the cultured cells have been shown to produce ACE in vitro (7). We therefore chose cultured endothelial cells as a convenient system to investigate the regulation of ACE production.…”