Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species

Pometto, Anthony L.; Lee, B T; Johnson, Kenneth E.

doi:10.1128/aem.58.2.731-733.1992

Cited by 129 publications

(29 citation statements)

References 6 publications

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“…However, some works5–7 have demonstrated that ligninolytic and cellulolytic fungi ( Phanerochaete chrysosporium , Aspergillus niger , Penicillium pinophilum , Gliocadium virens , Paecilomyces variotti ) can degrade oxidized PE products. Pometto et al8 found an extracellular enzyme in a Streptomyces sp. extract, able to attack and modify thermo‐oxidized PE‐starch films (70°C, 10 days) after three weeks of treatment.…”

Section: Introductionmentioning

confidence: 99%

Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger

Volke-Sepúlveda

Saucedo‐Castañeda

Gutiérrez-Rojas

et al. 2001

J of Applied Polymer Sci

171

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Differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were used to determine morphological, structural and surface changes (biodegradation) on thermo-oxidized (80°C, 15 days) low-density polyethylene (TO-LDPE) incubated with Aspergillus niger and Penicillium pinophilum fungi, with and without ethanol as cosubstrate for 31 months. TO-LDPE mineralization by fungi was also evaluated. Significantly morphological and structural final changes on biologically treated TO-LDPE samples were observed. Decreases to three units on crystallinity and crystalline lamellar thickness (0.4 -1.8 Å), and increases in small-crystals content (up to 3.2%) and mean crystallite size (8.4 -14 Å) were registered. An oxidation decrease (almost twice) on samples without ethanol with respect to the control was observed, while in those with ethanol it was increased (up to 2.5 times). Double bond index increased more than twice from 21 to 31 months. The higher TO-LDPE changes and fungi-LDPE interaction was observed in samples with ethanol, suggesting that ethanol favors the TO-LDPE biodegradation, at least in case of P. pinophilum, probably by means of a cometabolic process. Mineralization of 0.50 % and 0.57 % for A. niger, and of 0.64 % and 0.37 % for P. pinophilum were obtained, for samples with and without ethanol, respectively. A model to explain morphological and structural changes on biologically treated TO-LDPE is also proposed.

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Section: Introductionmentioning

confidence: 99%

Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger

Volke-Sepúlveda

Saucedo‐Castañeda

Gutiérrez-Rojas

et al. 2001

J of Applied Polymer Sci

171

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“…Since this is not the case, the root cause for the differential and quick degradation of OPENac can only be attributed to the different biotic environment created by nanoclay. The results of some articles published in the literature on biodegradation of thermo‐oxidised OPE in soil and composting units also support this theory 32–34…”

Section: Resultsmentioning

confidence: 63%

Biodegradation of montmorillonite filled oxo‐biodegradable polyethylene

Reddy

Deighton

Bhattacharya

et al. 2009

J of Applied Polymer Sci

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Oxo-biodegradation of polyethylene has been well studied with different pro-oxidants and it has been shown that pro-oxidants have limited role in the oxidation of polyethylene and do not have any role in microbial growth. However, in few recent studies, montmorillonite clay has been reported to promote the growth of microbes by keeping the pH of the environment at levels conducive to growth. In an attempt to improve the overall oxo-biodegradation of polyethylene, montmorillonite nanoclay has been used in this study along with a prooxidant. Film samples of oxo-biodegradable polyethylene (OPE) and oxo-biodegradable polyethylene nanocomposite (OPENac) were subjected to abiotic oxidation followed by microbial degradation using microorganism Pseudomonas aeruginosa. The progress of degradation was followed by monitoring the chemical changes of the samples using high-temperature gel permeation chromatography (GPC) and infrared spectroscopy (FTIR). The growth of bacteria on the surface of the polymer was monitored using environmental scanning electron microscopy.GPC data and FTIR results have shown that the abiotic oxidation of polyethylene is influenced significantly by the pro-oxidant but not by nanoclay. But, the changes in molecular weight distribution and FTIR spectra for the biodegraded samples indicate that the growth rate of P. aeruginosa on OPENac is significantly greater than that on OPE. It indicates that nanoclay, by providing a favourable environment, helps in the growth of the microorganism and its utilisation of the polymer surface and the bulk of the polymer volume.

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“…have been utilized during the degradation of polyethylene for their effective biodegradation ability. [11][12][13][14] In a study by Nowak et al, Pseudomonas fluorescens was isolated from polyethylene surface. 15 It has also been found that P. fluorescens is able to form biofilm on the un-oxidized polyethylene surface during initial biodegradation process.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of polyethylene via complete solubilization by the action of Pseudomonas fluorescens, biosurfactant produced by Bacillus licheniformis and anionic surfactant

Mukherjee

RoyChaudhuri

Kundu

2017

J of Chemical Tech & Biotech

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BACKGROUND Long hydrocarbon (∼CH2) chain, absence of polar bonds and highly hydrophobic nature of polyethylene, make it one of most difficult environmental pollutants to degrade. In the present study, commercially available polyethylenes were treated with biosurfactant produced by Bacillus licheniformis, anionic surfactant and bacterially treated with Pseudomonas fluorescens in different combinations to achieve higher biodegradation. RESULTS Polyethylene was slightly oxidized by P. fluorescens in the first month and the oxidation of polyethylene induced by the biosurfactant during the second month was enhanced by the presence of lower amounts of carbonyl groups as observed in FTIR analysis. During the third month, highly oxidized polyethylene was entirely solubilized by the action of 10% SDS forming two‐layered liquid consisting of yellow oil‐like liquid as upper layer and an aqueous colourless lower layer. Biodegradable aliphatic acids, alcohols and short hydrocarbon molecules of 10–30 carbon chains were identified by GC–MS analysis in the aqueous solution. Weight loss of 7.13 ± 0.05% was also observed in polyethylene samples which were treated with SDS in the first month, then bacterially treated with P. fluorescens in the second month and finally treated with biosurfactant in the third month. CONCLUSIONS Polyethylene became biodegradable after complete solubilization in water by treating consecutively with Pseudomonas fluorescens in the first month, then with biosurfactant in the second month and finally treating with 10% sodium dodecyl sulphate (SDS) at 60 °C for the third month. Simultaneous treatment of polyethylene with P. fluorescens, surfactant and biosurfactant has a tremendous effect on the oxidation and biodegradation of polyethylene. © 2017 Society of Chemical Industry

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Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species

Cited by 129 publications

References 6 publications

Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger

Thermally treated low density polyethylene biodegradation by Penicillium pinophilum and Aspergillus niger

Biodegradation of montmorillonite filled oxo‐biodegradable polyethylene

Biodegradation of polyethylene via complete solubilization by the action of Pseudomonas fluorescens, biosurfactant produced by Bacillus licheniformis and anionic surfactant

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