Production of Adventitious Root Biomass and Bioactive Compounds from Hypericum perforatum L. Through Large Scale Bioreactor Cultures

Cui, Xi-Hua; Murthy, Hosakatte Niranjana; Paek, Kee-Yoeup

doi:10.1007/978-94-017-9223-3_11

Cited by 8 publications

(14 citation statements)

References 55 publications

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“…Finally, an airlift bioreactor sized 500 L was employed to further culture the adventitious roots. The results revealed that the pattern of adventitious root growth in the larger bioreactors was similar to that in smaller bioreactors (Cui et al 2014).…”

Section: Scale Up In Bioreactorsmentioning

confidence: 68%

“…Large-scale culture using the bioreactor system was also adopted for adventitious root culture of Hypericum perporatum L. In vitro grown adventitious roots were raised using leaf explants in MS solid medium and were then moved into shake flasks of 250 mL in size (Cui et al, 2014). Parameters including aeration volume, inoculum density, ammonium/nitrate ratio, sucrose concentration, and MS salt strength were evaluated to determine the best conditions for the adventitious root culture of this plant.…”

Section: Scale Up In Bioreactorsmentioning

confidence: 99%

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Adventitious root culture for secondary metabolite production in medicinal plants: A Review

Rahmat

Kang

2019

J Plant Biotechnol

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Medicinal plants are high-value natural resources that have been used as precautionary drugs by many people globally. The increasing global demand for bioactive compounds from medicinal plants has led to the overexploitation of many valuable species. One widely used approach to overcome this problem is the use of adventitious root cultures as a propagation strategy. This review examines the scientific research published globally on the application of adventitious root cultures for many medicinal plants. Adventitious roots generated under aseptic environments in suitable phytohormone-augmented medium exhibit high growth rates and production of important secondary metabolites. Parameters such as medium properties and composition, growth hormone type, and elicitation strategies for in vitro grown adventitious roots of medicinal plants, are the main topics discussed in this review. We also examine current developments in bioreactor system cultivation for plant bioactive compounds using adventitious root cultures, a technology with possible commercial applications, via several studies on adventitious root culture of medicinal plants in which bioreactor systems play a role. In conclusion, the development of adventitious root cultures for medicinal plants is highly useful because of their capability for vegetative propagation and germplasm preservation.

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Section: Scale Up In Bioreactorsmentioning

confidence: 68%

Section: Scale Up In Bioreactorsmentioning

confidence: 99%

Adventitious root culture for secondary metabolite production in medicinal plants: A Review

Rahmat

Kang

2019

J Plant Biotechnol

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“…The volumetric oxygen transfer coefficient ( k L a ) was taken as a criterion for the bioreactor hydrodynamics, where higher k L a values of the STBR and drum reactor were merits for the subsequent growth increase in these bioreactors . Consequently, STBRs turn out as future alternative for the cultivation of Hypericum root cultures instead of the commonly used airlift loop, bubble column, and mist bioreactors .…”

Section: Resultsmentioning

confidence: 99%

“…Ammonium was proven to be essential for the increase in the biomass [61,62]. However, other reports suggested that nitrate is superior over ammonium for the production process of P. notoginseng and H. perforatum [34,63]. Parallel to the ammonium consumption, values of electrical conductivity were also decreased.…”

Section: Responses Of the Root Cultures To Changes In Ph And Residualmentioning

confidence: 99%

Toward enhanced hyperforin production in St. John's wort root cultures

Gaid

Grosch

Möller

et al. 2019

Engineering in Life Sciences

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During the past decades, several trials targeted a stable, sustainable and economic production of St. John's wort (Hypericum perforatum) extract. The value of this extract stems from its use to treat depression and skin irritation due to its hyperforin content. Previously, hyperforin‐forming in vitro root cultures were established. Here, detailed growth and production kinetics have been analyzed over 40 days of cultivation. In the first 10 days, sucrose was completely hydrolyzed to glucose and fructose. The ammonium consumption supported the increase in the biomass and hyperforin production. When sucrose was replaced with glucose/fructose, the linear growth phase started 6 days earlier and resulted in a higher space‐time‐yield. The maximum hyperforin production was 0.82 mg L−1 day−1, which was 67 % higher than in the sucrose‐supplemented standard cultivation. Buffering the sucrose‐supplemented medium with phosphate caused a 2.7‐fold increase in the product to biomass yield coefficient. However, the combination of monosaccharides and buffering conditions did not cause an appreciable improvements in the production performance of the shake flask approaches. A potential scalability from flask to lab‐scale stirred bioreactors has been demonstrated. The results obtained offer a basis for a scalable production of hyperforin and a sustainable source for a tissue culture‐based phytomedicine.

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“…Little information is available on the effects of biofield on the total phenol contents of plants. Nevertheless, the amount of phenolic content detected in plants is known to be related to osmotic stress of plants under certain conditions (Cui, Murthy, & Paek, 2014). However, the factors causing the stress may vary widely depending on the type of plant and growing conditions.…”

confidence: 99%

Effect of Biofield Treatment on Growth and Physiology of Hydroponically-Grown Lettuce and Bok Choy Plants

Lee

2019

Agrivita.J.Agr.Sci

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The aim of this study was to investigate the effects of biofield treatments on the growth and physiology of lettuce and bok choy plants. Energy art pieces and water treated by energy art were used as biofield treatments. Overall, both the lettuce and bok choy plants exposed to a combination of energy art and energy art-treated water were significantly larger in terms of leaf and root growth than those in the other treatments. Clear improvements in the vegetative growth of these plants were found, as seen in the significantly higher number of leaves, leaf dry mass, and leaf area. Furthermore, the highest chlorophyll and carotenoid contents were detected in lettuces and bok choy cultivated in the energy art and energy art-treated water treatments, respectively, which were significantly higher than those grown in the control. Moreover, the photosynthetic efficiency of the lettuce and bok choy plants exposed to the energy treatments were improved significantly. The results of this study demonstrated significant effects of biofield in the form of energy art and energy art-treated water on hydroponically-grown lettuce and bok choy plants. Biofield treatments such as energy art can be used as an alternative approach to improve plant growth and yield.

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Production of Adventitious Root Biomass and Bioactive Compounds from Hypericum perforatum L. Through Large Scale Bioreactor Cultures

Cited by 8 publications

References 55 publications

Adventitious root culture for secondary metabolite production in medicinal plants: A Review

Adventitious root culture for secondary metabolite production in medicinal plants: A Review

Toward enhanced hyperforin production in St. John's wort root cultures

Effect of Biofield Treatment on Growth and Physiology of Hydroponically-Grown Lettuce and Bok Choy Plants

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