Production of a Sensitive Monoclonal Antibody to Sterigmatocystin and Its Application to ELISA of Wheat

Li, Suzhen; Chen, Pu Yan; Marquardt, R. R.; Zhengkang, Han; Clarke, J

doi:10.1021/jf950258m

Cited by 14 publications

(10 citation statements)

References 15 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only a very limited number of enzyme-linked immunoassay (ELISA) methods have been described for STC, making use of either polyclonal (Morgan et al, 1986;Olson and Chu, 1993a) or monoclonal antibodies (Li et al, 1996). In wheat, the lowest reproducible concentration of STC that could be quantified was 31 µg/kg, with a working range up to 250 µg/kg (Li et al, 1996).…”

Section: Immunochemical Methodsmentioning

confidence: 99%

Scientific Opinion on the risk for public and animal health related to the presence of sterigmatocystin in food and feed

2013

EFSA Journal

134

View full text Add to dashboard Cite

The European Food Safety Authority (EFSA) was asked by the European Commission to deliver a scientific opinion on sterigmatocystin (STC) in food and feed. STC is a polyketide mycotoxin that shares its biosynthetic pathway with aflatoxins. Following an EFSA call for data, analytical results from 247 food and 334 feed samples were submitted. In food, analytical results on STC were reported to be all below the limit of detection or limit of quantification. In feed, only four quantified results were reported. Therefore, the EFSA Panel on Contaminants in the Food Chain (CONTAM Panel) concluded that the available occurrence data are too limited to carry out a reliable human and animal dietary exposure assessment. Acute oral toxicity of STC is relatively low, and liver and kidneys are the target organs. STC is mutagenic in both bacterial and mammalian cells after metabolic activation and forms DNA adducts. Tumourigenicity has been observed after oral, intraperitoneal, subcutaneous and dermal administration resulting in hepatocellular carcinomas, haemangiosarcomas in the liver, angiosarcomas in brown fat and lung adenomas. Since no exposure data were available, the margin of exposure approach for substances that are genotoxic and carcinogenic could not be applied for STC, and thus the CONTAM Panel could not characterise the risk for human health. Regarding animals, the Panel noted that STC is hepatotoxic in poultry and pigs, and nephrotoxic in poultry and toxic in several fish species. However, in the absence of exposure data for livestock, fish and companion animals, and given the limited knowledge on the adverse effects of STC, the CONTAM Panel could not characterise the risk for animal health. More occurrence data on STC in food and feed need to be collected to allow dietary exposure assessment. For food, methods with a limit of quantification of less than 1.5 µg/kg should be applied. Analytical methods for STC in food and feed based on liquid chromatography-mass spectrometry (LC-MS) reach the lowest limits of detection (LOD). However, the sensitivity varies between individual matrices and whether a single or multi-analyte method is applied. So far, no certified reference materials are available for STC. In addition, no proficiency tests for STC analysis in food or feed could be identified.In the literature, only limited information is available on the occurrence of STC in different foods and feed. Most of the earlier studies using methods with rather high LODs/limits of quantification (LOQ) report a high percentage of left-censored data (often 100 %) on food and feed. Two recent studies using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with an LOD of 0.15 µg/kg and an LOQ of 0.3 µg/kg reported STC positive samples of bread and of grain intended for use as animal feed. While these data indicate that these methods are sufficiently sensitive to quantify STC in food and feed, the data were insufficient to use for a reliable exposure assessment.Moreover, STC has been occasionally reported t...

show abstract

Section: Immunochemical Methodsmentioning

confidence: 99%

Scientific Opinion on the risk for public and animal health related to the presence of sterigmatocystin in food and feed

2013

EFSA Journal

134

View full text Add to dashboard Cite

show abstract

“…) or lacked sensitivity (Li et al . ), and were obviously not further studied. More recently, Li et al .…”

Section: Introductionmentioning

confidence: 99%

“…Currently, rapid immunochemical screening tests, as available for the aflatoxins, do not exist. Approaches to establish enzyme immunoassays (EIA) based on antibodies against STC-hemiacetal as the antigen were reported some decades ago, but these either required tedious chemical conversion of STC into its hemiacetal before detection (Li and Chu 1984;Morgan et al 1986;Lou et al 1996) or lacked sensitivity (Li et al 1996), and were obviously not further studied. More recently, Li et al (2014) reported monoclonal antibodies against STC, employing a method originally described for AFB 2 by Cervinio et al (2007), and their use in competitive indirect EIA.…”

Section: Introductionmentioning

confidence: 99%

A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenicAspergillusspecies

Wegner

Bauer

Dietrich

et al. 2017

Lett Appl Microbiol

View full text Add to dashboard Cite

The carcinogenic mycotoxin sterigmatocystin (STC) is produced by several Aspergillus species, either alone or together with aflatoxins. Here, we report a very simple and straightforward procedure to obtain highly sensitive and specific anti-STC antibodies, and their use in the first ever real STC-specific competitive direct enzyme immunoassay (EIA). In combination with a previous EIA for aflatoxins, this study for the first time demonstrates the potential of a STC/aflatoxin EIA pair for what is branded as 'immunochemotaxonomic' identification of mycotoxigenic Aspergillus species. This new analytical tool enhances analytical possibilities for differential analysis of STC and aflatoxins.

show abstract

“…Li et al (1996) developed a sensitive monoclonal antibody for STC and applied it in an enzyme-linked immunosorbent assay (ELISA) for STC quantification in wheat. Among chromatographic methods, thin layer chromatography has been widely used as a technique for STC analysis (AOAC 2005;Versilovskis and De Saeger 2010).…”

Section: Introductionmentioning

confidence: 99%

Determination of sterigmatocystin in grain using gas chromatography-mass spectrometry with an on-column injector

Hossain

Goto

2014

Mycotoxin Res

View full text Add to dashboard Cite

Sterigmatocystin (STC) is a mycotoxin produced by several species of Aspergillus and other fungi. STC is a precursor of the carcinogen aflatoxin B1 and exerts carcinogenic, teratogenic, and mutagenic effects. A reliable GC-MS-based analytical method using on-column injection was developed and validated to determine STC content in grain. In this method, STC was extracted with acetonitrile (84%), and the filtered extract was diluted with phosphate buffer before immunoaffinity column (IAC) cleanup. After elution and N2 evaporation, the sample containing STC was dissolved in acetone and injected into the GC-MS system without derivatization. Separation of STC was carried out by a capillary column (0.25 mm i.d.×30 m, 0.25 μm) with a deactivated pre-column (0.53 mm i.d.×0.6 m). The matrix effect was investigated in maize, wheat, and rice, and an insignificant matrix effect was observed after IAC cleanup. The calibration curve was linear in the range of 20-300 pg (equivalent to 8-120 μg/kg in grain) with a coefficient of determination (R2) of 0.999, and the relative standard deviation of repeatability (RSDr) was less than 10 %. The limit of detection (LOD) of the method was 6 pg (equivalent to 2.4 μg/kg in grain), and the limit of quantification (LOQ) was 20 pg (equivalent to 8 μg/kg). The method described in this report is sensitive, reliable, and can be used to monitor STC contamination in grain.

show abstract

Production of a Sensitive Monoclonal Antibody to Sterigmatocystin and Its Application to ELISA of Wheat

Cited by 14 publications

References 15 publications

Scientific Opinion on the risk for public and animal health related to the presence of sterigmatocystin in food and feed

Scientific Opinion on the risk for public and animal health related to the presence of sterigmatocystin in food and feed

A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenicAspergillusspecies

Determination of sterigmatocystin in grain using gas chromatography-mass spectrometry with an on-column injector

Contact Info

Product

Resources

About