In the last decade, near-infrared (NIR) and mid-infrared (MIR) spectroscopy have proven to be most promising tools for the detection of fungal contamination and estimation of mycotoxins in agricultural commodities, particularly of cereals. Owing to significant economic losses incurred from fungal contamination of foodstuffs, producers and processors are looking for fast, reliable, and less-expensive methods for the detection of fungal damage. In this context, NIR and MIR spectroscopy offer a fast, less-expensive, non-destructive, and relatively simple analytical method. Results from published studies indicate that NIR and MIR spectroscopy can be successfully applied to identifying fungal contamination and estimating specific mycotoxins. This review will focus on the applications of NIR and MIR spectroscopy to the classification of fungal contamination and the determination of specific mycotoxin contamination levels, and to compare this technology with traditional analytical methods.
The mycotoxin sterigmatocystin (STC) is produced mainly by some Aspergillus and Penicillium fungi; it naturally contaminates cereals, peanuts, and products derived from these crops, and is both mutagenic and carcinogenic. As an intermediate of aflatoxin (AF) biosynthesis, its structure is similar to that of AF. Although immunoaffinity columns (IACs) are a popular approach to sample clean-up, no IAC is commercially available for STC, but a commercially available IAC for AF shows cross reactivity to STC. We here developed a new method for analyzing STC in grains using such an IAC and liquid chromatography mass spectrometry (LCMS), and validated this method using six different grains. The STC limit of detection (signal-to-noise ratio, S/N = 3) was 2.5 pg (1.0 μg/kg in the product), and the calibration curve was linear in the range of 7.5-375 pg (3.0-150 μg/kg in the product). The within-day recovery of STC from samples spiked with STC at 5.0 and 50 μg/kg was 83.2-102.5% and the RSDr (relative standard deviation of repeatability) of these samples was 1.9-6.5%; the RSDr of STC-pretreated grain samples was 3.1-14.0%. Average recovery of STC from samples spiked with STC in the range of 5.0-100 μg/kg STC was 83.2-102.5%, with an RSDr of 0.24-6.5%; the RSDr of STC-pretreated grain samples was 2.4-14.0%. In an intermediate precision study, the average STC recovery from STC-spiked samples by three analysts was 95.2-107.5%, with RSDRi (intermediate precision) of 4.0-7.1%; the RSDRi of the STC-pretreated samples was 4.8-10.4%. Thus, the proposed method was effective for STC analysis in grains, and holds potential for a novel application of a commercial IAC, intended for AFs, in STC analysis.
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