Production of a novel α-amylase by Bacillus atrophaeus NRC1 isolated from honey: Purification and characterization

Abdelaziz, Ahmed; Karam, Eman A.; Ghanem, Manal M.E.; Moharam, Maysa E.; Kansoh, Amany L.

doi:10.1016/j.ijbiomac.2020.01.120

Cited by 34 publications

(17 citation statements)

References 29 publications

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“…Under optimum medium components and culture conditions, the maximum amylase activity reached 173.014 ± 11.918 U/ml. Under laboratory culture conditions, the maximum yield of a novel α‐amylase by Bacillus atrophaeus NRC1 isolated from honey ranged from 20 to 30 U/ml (Abd‐Elaziz, Karam, Ghanem, Moharam, & Kansoh, 2020). A strain of Bacillus lichenformis , which could produce α‐amylase, was selected from the soil, and its fermentation broth amylase activity was determined to be about 75 U/ml (Kannan & Kanagaraj, 2019).…”

Section: Discussionmentioning

confidence: 99%

A high amylase‐producing bacterial strain exhibiting the phosphorus‐dissolving ability, isolated from freshwater aquaculture pond

et al. 2020

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Amylase‐producing bacteria could improve water quality contaminated by waste from feed residue and fish metabolism, thereby increasing the efficiency of aquaculture systems. The objective of this research was to screen and optimize fermentation conditions of a high amylase‐producing strain. Four amylase‐producing bacterial strains (named S458‐1, G05, H38 and B09) were isolated from a grass carp pond, and the strain S458‐1 showed the highest amylase‐producing ability, with 19.58 ± 0.38 mm hydrolysis circle diameter. The strain S458‐1 was identified as Bacillus cereus based on morphological identification, biochemical identification and 16S rDNA sequence analysis. The optimal culture medium formula included (in g/L) Ca2+ 0.8, Mg2+ 0.2, Mn2+ 0.4, Fe2+ 0.6, Al3+ 0.2, 1% soluble starch and 1% peptone. The optimal fermentation conditions were determined as initial pH 9, culture temperature 37°C, fermentation time of 60 hr and 2% inoculum. Under the optimal formula and condition, its enzyme activity increased from 32 U/ml to 173.01 U/ml, a 5.41‐fold increase. Surprisingly, our research found that the strain S458‐1 also had phosphorus degradation capabilities. Its phosphorus‐dissolving ability was both time‐ and concentration‐dependent. Thus, this study will make a contribution to the bacterial amylase based on the fermentation process and provide a theoretical basis for further research of aquatic probiotics.

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Section: Discussionmentioning

confidence: 99%

A high amylase‐producing bacterial strain exhibiting the phosphorus‐dissolving ability, isolated from freshwater aquaculture pond

et al. 2020

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“…Ozdemir et al [21] produced Bacillus mojavensis microorganism at 35°C, pH 7.0 and 36 hours for use in biotechnological studies. In their studies on Bacillus atrophaeus NRC1, Abd-Elaziz et al [22] determined the production conditions of the bacteria as pH 6.0, 40 °C and 24 hours.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer: Obtaining and Characterization of α-Amylase

Enez¹

2020

Adıyaman University Journal of Science

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α-Amylase (1,4-α D-glucanohydrolase; EC 3.2.1.1) is one of the enzymes that can be used in a number of industrial processes such as bakery, textile, paper, detergents, and is the most important industrial product that plays an important role in the production of bioprotein and the fermentation of starch. The present research aims to the information gap in this area by performing bacterial isolation from the root of Astragalus gummifer. Bacterial isolation was performed using serial dilution technique. 16S rRNA analysis was performed for species identification of the microorganism. Isolated microorganism was determined to be gram (+), moving as a result of biochemical tests. Biochemical tests; Catalase, hemolysis, glucose and lactose tests positive (+); indole, H2S and urease tests showed negative (-) results. Soil samples were analyzed by ICP-MS.As a result of the analysis, it was determined that Al, Cr, Cu, Co, Fe, Pb, Mn and Zn were found in excess amount in the isolated bacteria. The optimal reproductive function of the bacteria was determined at 72 hours, 30 o C and pH 7.0. The maximum production time of α-amylase from Bacillus sp. was 24 hours, 30 o C and pH 7.0. Bacteria identified and found to produce amylase were determined to be used in different biotechnological areas.

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“…Based on 16S rRNA sequencing, microbial analysis was carried out according to the reported methods ( Abd-Elaziz et al, 2020 ). DNA was extracted based on the instructions of the Omega Kit e.z.n.a TM Mag-Bind SOIL DNA Kit ( http://omegabiotek.com/store/product/soil-dna-kit/ ).…”

Section: Methodsmentioning

confidence: 99%

Spirulina polysaccharide induces the metabolic shifts and gut microbiota change of lung cancer in mice

Peng

Lin

et al. 2022

Current Research in Food Science

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Production of a novel α-amylase by Bacillus atrophaeus NRC1 isolated from honey: Purification and characterization

Cited by 34 publications

References 29 publications

A high amylase‐producing bacterial strain exhibiting the phosphorus‐dissolving ability, isolated from freshwater aquaculture pond

A high amylase‐producing bacterial strain exhibiting the phosphorus‐dissolving ability, isolated from freshwater aquaculture pond

Isolation and Identification of Bacillus sp. from Root Soil of the Astragalus gummifer: Obtaining and Characterization of α-Amylase

Spirulina polysaccharide induces the metabolic shifts and gut microbiota change of lung cancer in mice

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