Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

Qin, Jiayang; Xiao, Zijun; Ma, Cuiqing; Xie, Neng-Zhong; Liu, Peihai; Xu, Ping

doi:10.1016/s1004-9541(06)60050-5

Cited by 92 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The maximum speciWc growth rate was 0.2/h, and this was the same as that obtained during extractive fermentation in the presence of oleyl alcohol. The cell yield was approximately 0.10 g/g glucose, while the 2,3-butanediol yield was 0.30 g/ g, nearly indicated to that obtained by Qin et al [16]. The 2,3-butanediol productivity was 0.5 g/l h.…”

Section: Fermentationsupporting

confidence: 56%

In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction

Anvari

Khayati

2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

End-product conversion, low product concentration and large volumes of fermentation broth, the requirements for large bioreactors, in addition to the high cost involved in generating the steam required to distil fermentation products from the broth largely contributed to the decline in fermentative products. These considerations have motivated the study of organic extractants as a means to remove the product during fermentation and minimize downstream recovery. The aim of this study is to assess the practical applicability of liquid-liquid extraction in 2,3-butanediol fermentations. Eighteen organic solvents were screened to determine their biocompatibility, and bioavailability for their effects on Klebsiella pneumoniae growth. Candidate solvents at first were screened in shake flasks for toxicity to K. pneumoniae. Cell density and substrate consumption were used as measures of cell toxicity. The possibility of employing oleyl alcohol as an extraction solvent to enhance end product in 2,3-butanediol fermentation was evaluated. Fermentation was carried out at an initial glucose concentration of 80 g/l. Oleyl alcohol did not inhibit the growth of the fermentative organism. 2,3-Butanediol production increased from 17.9 g/l (in conventional fermentation) to 23.01 g/l (in extractive fermentation). Applying oleyl alcohol as the extraction solvent, about 68% of the total 2,3-butanediol produced was extracted.

show abstract

Section: Fermentationsupporting

confidence: 56%

In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction

Anvari

Khayati

2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Esse alto rendimento, associado ao rápido crescimento em meios de cultivo simples, tem apontado o gênero K. oxytoca como de grande potencial biotecnológico. 26,35,37 Além da espécie oxytoca, até o momento K. pneumoniae e P. polymyxa têm sido as mais empregadas na produção eficiente de 2,3-BD. Aerogenes enterobacter e Serratia marcescens também são considerados potenciais micro-organismos, no entanto, as pesquisas com estes são menos relatadas na literatura.…”

Section: Condições Operacionais E Variáveis Envolvidas Na Produção Dounclassified

Produção biotecnológica de produtos de valor agregado utilizando glicerol residual proveniente da síntese de biodiesel

Silva

Souza

Antero

2017

evid.

View full text Add to dashboard Cite

ResumoO aumento significativo na produção de biodiesel por transesterificação de óleos comestíveis e não comestíveis causaram excedente de glicerol no mercado. Com suas características, estrutura química única, renovabilidade e biodisponibilidade, o glicerol tem promissor potencial para ser transformado em produtos químicos de alto valor agregado. Este artigo oferece uma análise abrangente e crítica do tratamento biotecnológico do glicerol a 1,3-propanodiol e 2,3-butanodiol. A conversão microbiana do glicerol apresenta vantagens em relação às sínteses químicas requerendo menor número de etapas, temperaturas medianas e pressão do ambiente, além de não gerar intermediários tóxicos.Palavras-chave: Micro-organismos. Biodiesel. Transesterificação. Fermentação de glicerol.

show abstract

“…During the last few decades, considerable efforts have been made to improve the production of 2,3-BD from fermentation, and much progresses have been achieved (Qin et al 2006;Syu 2001;Zeng et al 1991). However, major difficulties still exist in downstream processing because 2,3-BD has a highboiling point and a high affinity for water.…”

Section: Introductionmentioning

confidence: 99%

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system

2008

View full text Add to dashboard Cite

A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.

show abstract

Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

Cited by 92 publications

References 19 publications

In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction

In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction

Produção biotecnológica de produtos de valor agregado utilizando glicerol residual proveniente da síntese de biodiesel

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system

Contact Info

Product

Resources

About