Production of 2,3-butanediol by engineered Saccharomyces cerevisiae

where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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“…168 It is a fuel with a high heating value (27 000 J g − 1 ) and has been used as a liquid fuel or fuel additive.…”

Section: Biofuelsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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“…amounts of 2,3-BD (0.4-2.0 g/L) during wine fermentation (Ehsani et al, 2009) although S. cerevisiae possesses three putative pathways converting pyruvate to 2,3-BD (Romano and Suzzi, 1996). Metabolic engineering to produce 2,3-BD with engineered S. cerevisiae strains harboring the bacterial 2,3-BD biosynthetic pathway have been attempted (Kim et al, 2013;Ng et al, 2012). The bacterial 2,3-BD biosynthetic pathway converts pyruvate into 2,3-BD by three-step enzyme reactions: two molecules of pyruvate are condensed to produce a-acetolactate by acetolactate synthase, a-acetolactate is decarboxylated to produce acetoin by acetolactate decarboxylase, and acetoin is further reduced into 2,3-BD by butanediol dehydrogenase.…”

Section: Introductionmentioning

confidence: 99%

Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae

Kim

Seo

Zhang

et al. 2015

Bioresource Technology

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“…Several bacterial species including Klebsiella pneumoniae, K. variicola, K. oxytoca, Serratia marcescens and Enterobacter cloacae have been used to produce 2,3-BD with high yields through optimization of culture conditions or genetic engineering (Celinska and Grajek 2009; Kim et al 2013), but these strains are pathogenic or opportunistic pathogens and have been categorized under risk group-2 microorganisms, unsuitable for industrial-scale biotransformation (Ji et al 2011;Kim et al 2013). The microorganisms that may cause disease in humans and animals but are unlikely to be a serious hazard to laboratory personnel, the community, animals or the environment are called risk group 2.…”

Section: Introductionmentioning

confidence: 99%

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Rahman

Qin

2018

Bioresour. Bioprocess.

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Background:The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate.Results: A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli-K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/ mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. Conclusions:The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.

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Production of 2,3-butanediol by engineered Saccharomyces cerevisiae

Cited by 100 publications

References 35 publications

Production of valuable compounds by molds and yeasts

Production of valuable compounds by molds and yeasts

Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

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