Production of 15-F-isoprostane as an assessment of oxidative stress in dairy cows at different stages of lactation

Abstract

Oxidative stress contributes to dysfunctional immune responses and predisposes dairy cattle to several metabolic and inflammatory-based diseases. Although the negative effects of oxidative stress on transition cattle are well established, biomarkers that accurately measure oxidative damage to cellular macromolecules are not well defined in veterinary medicine. Measuring 15-F-isoprostane, a lipid peroxidation product, is the gold standard biomarker for quantifying oxidative stress in human medicine. The aim of our study was to determine whether changes in 15-F-isoprostane concentrations in plasma and milk could accurately reflect changes in oxidant status during different stages of lactation. Using liquid chromatography-tandem mass spectrometry, 15-F-isoprostane concentrations were quantified in milk and plasma of 12 multiparous Holstein-Friesian cows that were assigned to 3 different sampling periods, including the periparturient period (1-2 d in milk; n = 4), mid lactation (80-84 d in milk; n = 4), and late lactation (183-215 d in milk; n = 4). Blood samples also were analyzed for indicators of oxidant status, inflammation, and negative energy balance. Our data revealed that 15-F-isoprostane concentrations changed at different stages of lactation and coincided with changes in other gauges of oxidant status in both plasma and milk. Interestingly, milk 15-F-isoprostane concentrations and other indices of oxidant status did not follow the same trends as plasma values at each stage of lactation. Indeed, during the periparturient period, systemic 15-F-isoprostane increased significantly accompanied by an increase in the systemic oxidant status index. Milk 15-F-isoprostane was significantly decreased during the periparturient period compared with other lactation stages in conjunction with a milk oxidant status index that trended lower during this period. The results from this study indicate that changes in 15-F-isoprostane concentrations in both milk and plasma may be strong indicators of an alteration in redox status both systemically and within the mammary gland.

Colostrum supplementation with n-3 fatty acids and α-tocopherol alters plasma polyunsaturated fatty acid profile and decreases an indicator of oxidative stress in newborn calves

“…An antioxidant-reducing agent of 50% methanol, 25% ethanol, and 25% water with 0.9 mM butylhydroxytoluene, 0.54 mM EDTA, 3.2 mM triphenylphosphine, and 5.6 mM indomethacin, as described in Kuhn et al (2018), was added at 20 µL to 125 µL of thawed colostrum. Samples underwent lipid hydrolysis via the addition of 178 µL of KOH and incubating for 45 min at 45°C.…”

“…Then, 6 M HCl was added to the removed supernatant in increments of 10 µL until the supernatant pH was decreased to 4 or less. An internal standard mixture of 15 µL was added before undergoing solid-phase extraction with Oasis HLB 12-cm 3 LP extraction columns (Waters, Milford, MA) via a Biotage (Charlotte, NC) ExtraHera, further described in Kuhn et al (2018). Samples were then dried in a Savant SpeedVac (Thermo Fisher Scientific, Waltham, MA) and reconstituted in 1.5:1 methanol: HPLC water.…”

Cow's milk: Composition, nutritional, biological and cardioprotective benefits

“…Les quelques études réalisées chez les rongeurs tendent à montrer que le lait et ses protéines peuvent jouer un rôle protecteur contre une augmentation du stress oxydant [28,34]. La capacité antioxydante du lait (vache, buffle) est, en grande partie, due aux vitamines A, E, aux caroténoïdes, au zinc, au sélénium et au système de défense anti-oxydante enzymatique (superoxyde dismutase, catalase, glutathion peroxidase).…”

Effects of Prepartally Supplements of Vitamins and Trace Elements on Total Antioxidant and Oxidant Status and Udder Health Parameters on Day 14 Postpartum in Holstein-Friesian Cows

“…But, when the production of free radicals exceeds the antioxidants defense capacity, oxidative stress occurs (Batistel et al 2018). It has been reported that oxidative stress impairs cells' structures (especially immune cells) by damaging their macro components such as DNA, lipid and protein (Kuhn et al 2018). In addition, it is known that excessive oxidative stress can cause pathologic disorders of tissue and organ.…”